中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
5期
562-564
,共3页
疼痛,手术后%星形细胞%脊髓
疼痛,手術後%星形細胞%脊髓
동통,수술후%성형세포%척수
Pain,postoperative%Astroctyes%Spinal cord
目的 探讨持续性术后痛大鼠脊髓背角星形胶质细胞的活化.方法 成年雄性SD大鼠48只,体重200～250 g,随机分为2组(n=24):假手术组和模型组.模型组采用皮肤肌肉切口牵拉术制备持续性术后痛模型,假手术组仅暴露内收肌,不牵拉皮肤肌肉组织.分别于模型制备前(基础状态)、制备后1、3、12、22和32 d时测定大鼠机械缩足阈值(MWT).各时点MWT测定结束后随机取4只大鼠,处死后采用免疫组织荧光法测定脊髓胶质纤维酸性蛋白(GFAP)的表达水平.结果 与基础值比较,假手术组各时点MWT和脊髓GFAP表达水平差异无统计学意义(P＞0.05),模型组模型制备后1～22 d MWT降低,12 d时最低,模型制备后3～22 d脊髓GFAP表达上调,12 d时达峰值(P＜0.05或0.01);与假手术组比较,模型组MWT降低,脊髓GFAP表达上调(P＜0.05).结论 大鼠持续性术后痛与脊髓星形胶质细胞的活化有关.
目的 探討持續性術後痛大鼠脊髓揹角星形膠質細胞的活化.方法 成年雄性SD大鼠48隻,體重200～250 g,隨機分為2組(n=24):假手術組和模型組.模型組採用皮膚肌肉切口牽拉術製備持續性術後痛模型,假手術組僅暴露內收肌,不牽拉皮膚肌肉組織.分彆于模型製備前(基礎狀態)、製備後1、3、12、22和32 d時測定大鼠機械縮足閾值(MWT).各時點MWT測定結束後隨機取4隻大鼠,處死後採用免疫組織熒光法測定脊髓膠質纖維痠性蛋白(GFAP)的錶達水平.結果 與基礎值比較,假手術組各時點MWT和脊髓GFAP錶達水平差異無統計學意義(P＞0.05),模型組模型製備後1～22 d MWT降低,12 d時最低,模型製備後3～22 d脊髓GFAP錶達上調,12 d時達峰值(P＜0.05或0.01);與假手術組比較,模型組MWT降低,脊髓GFAP錶達上調(P＜0.05).結論 大鼠持續性術後痛與脊髓星形膠質細胞的活化有關.
목적 탐토지속성술후통대서척수배각성형효질세포적활화.방법 성년웅성SD대서48지,체중200～250 g,수궤분위2조(n=24):가수술조화모형조.모형조채용피부기육절구견랍술제비지속성술후통모형,가수술조부폭로내수기,불견랍피부기육조직.분별우모형제비전(기출상태)、제비후1、3、12、22화32 d시측정대서궤계축족역치(MWT).각시점MWT측정결속후수궤취4지대서,처사후채용면역조직형광법측정척수효질섬유산성단백(GFAP)적표체수평.결과 여기출치비교,가수술조각시점MWT화척수GFAP표체수평차이무통계학의의(P＞0.05),모형조모형제비후1～22 d MWT강저,12 d시최저,모형제비후3～22 d척수GFAP표체상조,12 d시체봉치(P＜0.05혹0.01);여가수술조비교,모형조MWT강저,척수GFAP표체상조(P＜0.05).결론 대서지속성술후통여척수성형효질세포적활화유관.
Objective To investigate the glial activation in the spinal cord in a rat model of persistent postoperative pain. Methods Forty-eight adult male SD rats weighing 200-250 g were randomly divided into 2 groups ( n = 24 each): group Ⅰ sham operation (group S) and group Ⅱ persistent postoperative pain. Persistent postoperative pain was evoked by skin/muscle incision and retraction (SMIR) as described by Flatters. Pawwithdrawal threshold to yon Frey hair stimulation was measured before operation (baseline) and at 1, 3, 12, 22and 32 d after establishment of the model. Four animals were sacrificed at each time point and lumbar segment of the spinal cord was removed for determination of expression of glial fibrillary acidic protein (GFAP) in the astrocytes by immunofluorescence histo-chemistry assay. Results The mechanical threshold started to decrease at 1 d after establishment of the model, and peaked at 12 d after establishment of the mode. Immunofluorescence histochemistry assay demonstrated that GFAP expression in the dorsal horn was significantly increased at 3 d after estabhshment of the model and reached the peak at 12 d and was maintained at the high level until 22 d after establishment of the model. Conclusion Glial activation is involved in the mechanism of persistent postoperative pain evoked by SMIR.
