CAJ | 학술논문

目的探讨神经病理性痛大鼠背根神经节(DRG) 孔钾离子通道TRESK mRNA表达的变化.方法雄性SD大鼠32只,体重22、0～250 g,采用随机数字表法,将大鼠随机分为2组(n=16):假手术组(S组)和神经病理性痛组(NP组).采用坐骨神经分支选择性损伤法制备神经病理性痛模型.S组仅暴露神经,不结扎.于术前1 d和术后1、3、5、7、14 d取8只大鼠,测定左后肢机械缩足反应阈值(MWT)和热缩足潜伏期(TWL).于术前1 d和术后14 d痛阈测定结束后取L4,5术侧DRG,采用RT-PCR法测定TRESK mRNA的表达.结果与S组比较,NP组MWT明显降低,DRG TRESK mRNA表达明显下调(P<0.05或0.01),TWL差异无统计学意义(P>0.05).结论神经病理性痛大鼠DRG TRESK mRNA表达下调,该变化可能与神经病理性痛的形成有关.
목적 탐토신경병이성통대서배근신경절(DRG) 공갑리자통도TRESK mRNA표체적변화.방법 웅성SD대서32지,체중22、0～250 g,채용수궤수자표법,장대서수궤분위2조(n=16):가수술조(S조)화신경병이성통조(NP조).채용좌골신경분지선택성손상법제비신경병이성통모형.S조부폭로신경,불결찰.우술전1 d화술후1、3、5、7、14 d취8지대서,측정좌후지궤계축족반응역치(MWT)화열축족잠복기(TWL).우술전1 d화술후14 d통역측정결속후취L4,5술측DRG,채용RT-PCR법측정TRESK mRNA적표체.결과 여S조비교,NP조MWT명현강저,DRG TRESK mRNA표체명현하조(P<0.05혹0.01),TWL차이무통계학의의(P>0.05).결론 신경병이성통대서DRG TRESK mRNA표체하조,해변화가능여신경병이성통적형성유관.
Objective To evaluate the changes in the expression of diplopore potassium ion channel TRESK mRNA in dorsal root ganlion (DRG) in rats with neuropathic pain (NP) .Methods Thirty-two male SD rats weighing 220-250 g were randomly divided into 2 groups ( n = 16 each) : group sham operation (group S) and group NP. NP was induced by ligation and severance of left tibial and common fibular nerves according to the technique described by Decosterd. Eight rats in each group were sacrificed 1 day before and 14 day after operation and their L4,5 DRGs in the operated side were isolated for determination of TRESK mRNA expression by RT-PCR. In the remaining 8 rats in each group paw withdrawal threshold to mechanical stimuli ( MWT) and paw withdrawal latency to a thermal nociceptive stimulus (TWL) were measured at 1 day before (baseline) and 1, 3, 5, 7, 14 day after operation. Results MWT was significantly lower in group NP than in group S. The TRESK mRNA expression in L4,5 DRGs in the operated side was significantly decreased after operation as compared with the baseline before operation in group NP and was significantly lower in group NP than in group S. Conclusion The development and maintenance of NP may be closely related with down-regulation of TRESK mRNA.