中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
1期
24-29
,共6页
王珍燕%江雪艳%张云智%卢洪洲
王珍燕%江雪豔%張雲智%盧洪洲
왕진연%강설염%장운지%로홍주
HIV感染%胞嘧啶%脱氨酶类%基因表达%单核细胞%抗原%CD4%淋巴细胞计数
HIV感染%胞嘧啶%脫氨酶類%基因錶達%單覈細胞%抗原%CD4%淋巴細胞計數
HIV감염%포밀정%탈안매류%기인표체%단핵세포%항원%CD4%림파세포계수
HIV infections%Cytosines Ammonia-lyases%Gene expression%Monocytes%Antigens%CD4%Lymphocyte count
目的 定量研究HIV-1感染者外周血单个核细胞(PBMC)中胞嘧啶脱氨基酶家族成员hA3B、hA3F和hA3G的mRNA表达水平,并分析它们与CD4-T淋巴细胞计数的相关性.方法 采集各21例未接受和已接受抗病毒治疗的HIV-1感染者、10例HIV-1阴性健康对照者的外周静脉血,Ficoll密度梯度离心法分离PBMC,采用反转录一实时荧光定量PCR法检测其中hA3B、hA3F和hA3G mRNA水平,流式细胞术检测CD4~+ T淋巴细胞计数.数据行t、t'或Wilcoxon秩和检验.结果 未治疗及已治疗的HIV-1感染者、HIV-1阴性者hA3B mRNA的表达水平分别为208.4、365.2和563.6(P＜0.05),hA3F tuRNA分别为245.5、316.6和442.9(P＜0.05),hA3GmRNA分别为404.6、360.8和638.6.HIV-1感染者hA3G mRNA水平低于健康对照(Z=-2.451,Z=-2.493,均P＜0.05),但未治疗与已治疗HIV-1组间比较差异无统计学意义(Z=0.340,P=0.7342).HIV-1感染者hA3B、hA3F和hA3G mRNA表达水平与CD4+T淋巴细胞计数均无相关性(未治疗者:r=0.0104,r=-0.0545,r=0.1623,均P＞0.05;已治疗者:r=0.3220,r=0.2193,r=0.1455,均P＞0.05).hA3B、hA3F和hA3G三者mRNA水平在已治疗HIV-1感染者和健康对照者中呈正相关(P＜0.05),而在未治疗HIV-1感染者无相关性(P＞0.05).结论 hA3B、hA3F和hA3G基因表达水平与CD4~+ T淋巴细胞计数无直接相关性;HIV-1感染可导致PBMC中hA3B、hA3F和hA3G表达水平降低,抗病毒治疗可上调hA3B和hA3F的表达.
目的 定量研究HIV-1感染者外週血單箇覈細胞(PBMC)中胞嘧啶脫氨基酶傢族成員hA3B、hA3F和hA3G的mRNA錶達水平,併分析它們與CD4-T淋巴細胞計數的相關性.方法 採集各21例未接受和已接受抗病毒治療的HIV-1感染者、10例HIV-1陰性健康對照者的外週靜脈血,Ficoll密度梯度離心法分離PBMC,採用反轉錄一實時熒光定量PCR法檢測其中hA3B、hA3F和hA3G mRNA水平,流式細胞術檢測CD4~+ T淋巴細胞計數.數據行t、t'或Wilcoxon秩和檢驗.結果 未治療及已治療的HIV-1感染者、HIV-1陰性者hA3B mRNA的錶達水平分彆為208.4、365.2和563.6(P＜0.05),hA3F tuRNA分彆為245.5、316.6和442.9(P＜0.05),hA3GmRNA分彆為404.6、360.8和638.6.HIV-1感染者hA3G mRNA水平低于健康對照(Z=-2.451,Z=-2.493,均P＜0.05),但未治療與已治療HIV-1組間比較差異無統計學意義(Z=0.340,P=0.7342).HIV-1感染者hA3B、hA3F和hA3G mRNA錶達水平與CD4+T淋巴細胞計數均無相關性(未治療者:r=0.0104,r=-0.0545,r=0.1623,均P＞0.05;已治療者:r=0.3220,r=0.2193,r=0.1455,均P＞0.05).hA3B、hA3F和hA3G三者mRNA水平在已治療HIV-1感染者和健康對照者中呈正相關(P＜0.05),而在未治療HIV-1感染者無相關性(P＞0.05).結論 hA3B、hA3F和hA3G基因錶達水平與CD4~+ T淋巴細胞計數無直接相關性;HIV-1感染可導緻PBMC中hA3B、hA3F和hA3G錶達水平降低,抗病毒治療可上調hA3B和hA3F的錶達.
목적 정량연구HIV-1감염자외주혈단개핵세포(PBMC)중포밀정탈안기매가족성원hA3B、hA3F화hA3G적mRNA표체수평,병분석타문여CD4-T림파세포계수적상관성.방법 채집각21례미접수화이접수항병독치료적HIV-1감염자、10례HIV-1음성건강대조자적외주정맥혈,Ficoll밀도제도리심법분리PBMC,채용반전록일실시형광정량PCR법검측기중hA3B、hA3F화hA3G mRNA수평,류식세포술검측CD4~+ T림파세포계수.수거행t、t'혹Wilcoxon질화검험.결과 미치료급이치료적HIV-1감염자、HIV-1음성자hA3B mRNA적표체수평분별위208.4、365.2화563.6(P＜0.05),hA3F tuRNA분별위245.5、316.6화442.9(P＜0.05),hA3GmRNA분별위404.6、360.8화638.6.HIV-1감염자hA3G mRNA수평저우건강대조(Z=-2.451,Z=-2.493,균P＜0.05),단미치료여이치료HIV-1조간비교차이무통계학의의(Z=0.340,P=0.7342).HIV-1감염자hA3B、hA3F화hA3G mRNA표체수평여CD4+T림파세포계수균무상관성(미치료자:r=0.0104,r=-0.0545,r=0.1623,균P＞0.05;이치료자:r=0.3220,r=0.2193,r=0.1455,균P＞0.05).hA3B、hA3F화hA3G삼자mRNA수평재이치료HIV-1감염자화건강대조자중정정상관(P＜0.05),이재미치료HIV-1감염자무상관성(P＞0.05).결론 hA3B、hA3F화hA3G기인표체수평여CD4~+ T림파세포계수무직접상관성;HIV-1감염가도치PBMC중hA3B、hA3F화hA3G표체수평강저,항병독치료가상조hA3B화hA3F적표체.
Objective To quantitatively investigate the expression levels of the cytosine deaminases,hA3B,hA3F and hA3G in peripheral blood mononuclear cell(PBMC)of human immunodeficiency virus(HIV)infected patients and to analyze the correlation between cytosine deaminases expression and CD4~+ T lymphocyte counts. Methods Peripheral blood samples were collected from 21 HIV-infected subjects who didn't take antiretroviral therapy(ART-),21 HIV-infected subjects receiving ART(ART+),and 10 HIV-uninfected subjects. PBMC were isolated by Ficoll density gradient centrifugation, followed by RNA extraction and cDNA synthesis.hA3B,hA3F and hA3G mRNA levels were determined by real-time fluorescent quantitative polymerase chain reaction(PCR).CD4~+ T lymphocyte counts were determined using flow cytometry. Data were analyzed by t test, t' test or Wilcoxon rank sum test. Results In HIV-infected subjects without or with ART,HIV-uninfected subjects, the levels of hA3B mRNA were 208.4,365.2 and 563.6,hA3F mRNA were 245.5,316.6 and 442.9,hA3G mRNA were 404.6,360.8 and 638.6,respectively.hA3G mRNA level in HIV-infected subjects was lower than that in HIV-uninfected controls(P=0.0131),but there was no statistical difference between ART+ and ART-groups(P=0.7342).There were no correlations between hA3B,hA3F and hA3G mRNA levels and CD4+ T lymphocyte counts in either ART-or ART+HIV-infected subjects(ART-:r=-0.0104,r=-0.0545,r=0.1623,all P＞0.05;ART+:r=0.3220,r=0.2193,r=0.1455,all P＞0.05).In addition,hA3B,hA3F and hA3G mRNA levels were positively correlated with one another in ART-HIV-infected subjects and HIV-uninfected controls(P＜0.05),but not in ART-HIV-infected subjects(P＞0.05).Conclusions hA3B,hA3F and hA3G expression levels do not directly correlate with CD4~+ T lymphocyte counts in HIV-1-infected patients,hA3B,hA3F and hA3G expression levels in PBMCs tend to he decreased after HIV-1 infection, and ART may increase hA3B and hA3F mRNA expression.
