CAJ | 학술논문

目的评价参麦注射液后处理对大鼠心肌缺血再灌注损伤的影响.方法健康雄性SD大鼠32只,10～ 12周龄,体重240 ～ 260 g,采用随机数字表法,将其随机分为3组(n=12):假手术组(S组)、缺血再灌注组(I/R组)和参麦注射液后处理组(SPO组).I/R组和SPO组采用结扎左冠状动脉前降支30 min,再灌注120 min的方法制备大鼠心肌缺血再灌注模型；S组只穿线不结扎.于缺血30 min时I/R组静脉注射生理盐水9 ml/kg,SPO组静脉注射参麦注射液9 ml/kg.再灌注120 min时,腹主动脉采集血样,测定血清CK活性和cTnI浓度；然后处死大鼠,取心肌组织,观察病理学结果和细胞凋亡情况,计算心肌细胞凋亡指数,采用免疫组化法检测心肌细胞Bcl-2和Bax的蛋白表达.结果与S组比较,I/R组和SPO组血清CK活性和cTnI浓度升高,I/R组心肌细胞凋亡指数升高,Bcl-2蛋白表达下调,Bax蛋白表达上调,SPO组心肌细胞凋亡指数升高,Bcl-2和Bax的蛋白表达上调(P＜0.01)；与I/R组比较,SPO组血清CK活性和cTnI浓度降低,心肌细胞凋亡指数降低,Bcl-2蛋白表达上调,Bax蛋白表达下调(P＜0.01),病理学损伤减轻.结论参麦注射液后处理可减轻大鼠心肌缺血再灌注损伤,其机制可能与上调Bcl-2蛋白表达,下调Bax蛋白表达,抑制心肌细胞凋亡有关.
목적 평개삼맥주사액후처리대대서심기결혈재관주손상적영향.방법 건강웅성SD대서32지,10～ 12주령,체중240 ～ 260 g,채용수궤수자표법,장기수궤분위3조(n=12):가수술조(S조)、결혈재관주조(I/R조)화삼맥주사액후처리조(SPO조).I/R조화SPO조채용결찰좌관상동맥전강지30 min,재관주120 min적방법제비대서심기결혈재관주모형；S조지천선불결찰.우결혈30 min시I/R조정맥주사생리염수9 ml/kg,SPO조정맥주사삼맥주사액9 ml/kg.재관주120 min시,복주동맥채집혈양,측정혈청CK활성화cTnI농도；연후처사대서,취심기조직,관찰병이학결과화세포조망정황,계산심기세포조망지수,채용면역조화법검측심기세포Bcl-2화Bax적단백표체.결과 여S조비교,I/R조화SPO조혈청CK활성화cTnI농도승고,I/R조심기세포조망지수승고,Bcl-2단백표체하조,Bax단백표체상조,SPO조심기세포조망지수승고,Bcl-2화Bax적단백표체상조(P＜0.01)；여I/R조비교,SPO조혈청CK활성화cTnI농도강저,심기세포조망지수강저,Bcl-2단백표체상조,Bax단백표체하조(P＜0.01),병이학손상감경.결론 삼맥주사액후처리가감경대서심기결혈재관주손상,기궤제가능여상조Bcl-2단백표체,하조Bax단백표체,억제심기세포조망유관.
Objective To investigate the effects of postconditioning with Shenmai-injectio on myocardial ischemia-reperfusion (I/R) injury in rats.Methods Thirty-six healthy male SD rats aged 10-12 weeks weighing 240-260 g were randomly divided into 3 groups ( n =12 each):sham operation group (group S) ; myocardial I/R group and Shenmai-injectio postconditioning group (group SPO).Myocardial I/R was produced by ligation of the left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion in groups I/R and SPO.In group SPO Shenmai-injectio 9 ml/kg was injected iv at the end of 30 min ischemia.Blood samples were collected from abdominal aorta at the end of 120 min reperfusion for determination of serum CK activity and cTnI concentration.The animals were then sacrificed.Myocardial specimens were obtained for microscopic examination,detection of apoptosis and determination of myocardial Bcl-2 and Bax protein expression ( by immuno-histochemis-try).Results Myocardial I/R significantly increased serum CK activity,cTnI concentration,apoptotic index (percentage of apoptotic cells) and Bax protein expression and decreased Bcl-2 protein expression in group I/R as compared with group S.Shenmai-injectio postconditioning significantly attenuated I/R-induced above changes and ameliorated histo-pathological damage in group SPO as compared with group I/R.Conclusion Shenmai-injectiopostconditioning can reduce myocardial I/R injury by up-regulating Bcl-2 expression and down-regulating Bax expression,leading eventually to reduction in apoptosis.