CAJ | 학술논문

目的研究生长激素(GH)基因多态性与青少年特发性脊柱侧凸(AIS)发生发展的关系.方法本研究包括265例AIS患者及193名正常对照.在AIS患者组,记录其最大Cobb角.采用PCR-RFLP的方法对GH基因启动子区域多态性位点rs2854184进行基因分型.结果在AIS患者组,GH基因rs2854184多态性位点的3个基因型AA、AT、TT分别占38.3%,50.3%,11.4%,正常对照组分别占39.6%,50.2%,10.10k,2组比较差异无统计学意义.同样,在MS患者组rs2854184多态性位点的2个等位基因A、T分别占63.5%,36.5%,正常对照组分别占64.7%,35.3%,2组比较差异无统计学意义.另外在AIS组内,多态性位点rs2854184不同基因型所对应最大Cobb角分别是从33.8°±10.0°,AT 36.4°±15.0°,TT34.5°±9.1°,3者比较差异无统计学意义.结论 GH基因rs2854184位点多态性与AIS的发生发展没有明显关系.
목적 연구생장격소(GH)기인다태성여청소년특발성척주측철(AIS)발생발전적관계.방법 본연구포괄265례AIS환자급193명정상대조.재AIS환자조,기록기최대Cobb각.채용PCR-RFLP적방법 대GH기인계동자구역다태성위점rs2854184진행기인분형.결과 재AIS환자조,GH기인rs2854184다태성위점적3개기인형AA、AT、TT분별점38.3%,50.3%,11.4%,정상대조조분별점39.6%,50.2%,10.10k,2조비교차이무통계학의의.동양,재MS환자조rs2854184다태성위점적2개등위기인A、T분별점63.5%,36.5%,정상대조조분별점64.7%,35.3%,2조비교차이무통계학의의.령외재AIS조내,다태성위점rs2854184불동기인형소대응최대Cobb각분별시종33.8°±10.0°,AT 36.4°±15.0°,TT34.5°±9.1°,3자비교차이무통계학의의.결론 GH기인rs2854184위점다태성여AIS적발생발전몰유명현관계.
Objective To investigate whether the growth hormone gene (GH) promotor polymorphism (rs2854184) is associated with the occurrence or curve severity of adolescent idiopathic scoliosis (AIS). Methods Two hundred and sixty-five AIS patients and 193 normal controls were recruited. The maximum Cobb angles were recorded in AIS patients. PCR-RFLP was used for the genotyping. Results The genotype frequency distribution were AA 38.3%, AT50. 3%, TT11.4% in AIS patients and AA 39.6%, AT 50.2%, 10.1% TT in controls for the promotor polymorphism rs2854184 in GH gene. It was comparable between AIS and normal control. The allele frequency distribution was also comparable between AIS and normal control. It was 63.5% for allele A, 36. 5% for allele T in AIS patients and 64.7% for allele A, 35.3% for allele T in normal control. The mean maximum Cobb angle in AIS patients with AA, AT, TT genotypes were 33.8°±10.00, 36.4°±15.0°, 34.5°±9.1°, respectively, it was similar with each other. Conclusion The GH gene promoter polymorphiam is neither associated with the occurrence nor the curve severity of AIS.