中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
4期
356-360
,共5页
双价IgY%眼镜蛇毒%蝰蛇毒%交叉免疫
雙價IgY%眼鏡蛇毒%蝰蛇毒%交扠免疫
쌍개IgY%안경사독%호사독%교차면역
Bivalent IgY%Cobra venom%Viper venom%Cross immunity
目的 通过双价抗蛇毒鸡卵黄抗体(bivalent anti-snake venom immunoglobulin yolk,双价IgY)的制备及其相关特性研究,为多价抗蛇毒IgY的制备和应用奠定基础.方法 两种单一抗原(舟山眼镜蛇、圆斑蝰泰国亚种)按顺序依次交替注入单只鸡体内,水稀释法制备双价IgY;测定双价IgY效价(间接ELISA法)、交叉免疫特性(双向免疫扩散试验)及对溶膜活性(溶膜试验)、半数致死量(LD50)的中和作用.结果 初次免疫后第28-42天,以水稀释法提取的双价IgY对眼镜蛇毒及蝰蛇毒的效价分别为1:12 800和1:6400.双价IgY与眼镜蛇亚科、蝰业科6种蛇毒有明显的交叉免疫反应,而与蝮亚科4种蛇毒的交叉免疫反应不明显.双价IgY能显著降低眼镜蛇、蝰蛇毒对鸡卵黄膜的溶膜作用,也能显著延长眼镜蛇和蝰蛇伤小鼠的存活时间(P<0.05),同等剂量的双价lgY提高眼镜蛇伤存活率优于蝰蛇伤.结论 双价IgY能够显著中和眼镜蛇、蝰蛇毒的溶膜和致死活性,能有效保护机体免受眼镜蛇毒和蝰蛇毒的攻击.
目的 通過雙價抗蛇毒鷄卵黃抗體(bivalent anti-snake venom immunoglobulin yolk,雙價IgY)的製備及其相關特性研究,為多價抗蛇毒IgY的製備和應用奠定基礎.方法 兩種單一抗原(舟山眼鏡蛇、圓斑蝰泰國亞種)按順序依次交替註入單隻鷄體內,水稀釋法製備雙價IgY;測定雙價IgY效價(間接ELISA法)、交扠免疫特性(雙嚮免疫擴散試驗)及對溶膜活性(溶膜試驗)、半數緻死量(LD50)的中和作用.結果 初次免疫後第28-42天,以水稀釋法提取的雙價IgY對眼鏡蛇毒及蝰蛇毒的效價分彆為1:12 800和1:6400.雙價IgY與眼鏡蛇亞科、蝰業科6種蛇毒有明顯的交扠免疫反應,而與蝮亞科4種蛇毒的交扠免疫反應不明顯.雙價IgY能顯著降低眼鏡蛇、蝰蛇毒對鷄卵黃膜的溶膜作用,也能顯著延長眼鏡蛇和蝰蛇傷小鼠的存活時間(P<0.05),同等劑量的雙價lgY提高眼鏡蛇傷存活率優于蝰蛇傷.結論 雙價IgY能夠顯著中和眼鏡蛇、蝰蛇毒的溶膜和緻死活性,能有效保護機體免受眼鏡蛇毒和蝰蛇毒的攻擊.
목적 통과쌍개항사독계란황항체(bivalent anti-snake venom immunoglobulin yolk,쌍개IgY)적제비급기상관특성연구,위다개항사독IgY적제비화응용전정기출.방법 량충단일항원(주산안경사、원반호태국아충)안순서의차교체주입단지계체내,수희석법제비쌍개IgY;측정쌍개IgY효개(간접ELISA법)、교차면역특성(쌍향면역확산시험)급대용막활성(용막시험)、반수치사량(LD50)적중화작용.결과 초차면역후제28-42천,이수희석법제취적쌍개IgY대안경사독급호사독적효개분별위1:12 800화1:6400.쌍개IgY여안경사아과、호업과6충사독유명현적교차면역반응,이여복아과4충사독적교차면역반응불명현.쌍개IgY능현저강저안경사、호사독대계란황막적용막작용,야능현저연장안경사화호사상소서적존활시간(P<0.05),동등제량적쌍개lgY제고안경사상존활솔우우호사상.결론 쌍개IgY능구현저중화안경사、호사독적용막화치사활성,능유효보호궤체면수안경사독화호사독적공격.
Objective To prepare the bivalent immunoglobulin yolk (lgY) against eobra and viper venom and to detect its activities as the foundation for production and application of polyvalent . Methods The venom of Naja atra Cantor and Daboia russellii siamensis injected alternately into the leghorn hen. Biva-lent lgY was extraeted by water dilution. The biological activity of bivalent lgY were deteeted in several as-pects, sueh as the potency ( by indireet ELISA assay), the cross immunity ( by double immunodiffusion), the membrane lysis activity ( by experiments of vitelline membrane lysis) and 50% lethal activity ( LD50 ). Results Bivalent IgY was extracted from eggs yolk in 28-42 days after the first immunization. The titers of bivalent lgY against cobra and viper venom were 1:12 800 and 1: 6400. The cross immunologic reactions of bivalent IgY were found obviously with six kinds of snake venoms from Elapinae and Viperinae. There were not immunologic precipitation lines between bivalent IgY and four kinds of snake venoms from Crotalinae. Bi- valent lgY obviously deereased the vitelline membrane lysis activity of cobra and viper venom and prolonged the average survival time of mice with cobra or viper envenomation (P < 0.05). Moreover, with the same dose of bivalent IgY, the survival rate of mice with cobra venom envenomation was higher than those with vi-per venom envenomation. Conclusion Bivalent lgY could signifieantly neutralize biologieal activities of co-bra and viper venom, protect animals with cobra or viper envenomation.
