CAJ | 학술논문

目的通过实验观察溶血标本在酶联免疫吸附试验(ELISA)一步法和二步法中对乙型肝炎两对半(HBVM)检测结果的影响.方法用80例健康人(HBVM均为阴性)的血液标本,人为造成不同程度溶血,用ELISA一步法和二步法同时检测乙型肝炎病毒表面抗原(HBsAg)、乙型肝炎病毒表面抗体(抗-HBs)、乙型肝炎病毒e抗原(HBeAg)、乙型肝炎病毒e抗体(抗-HBe)、乙型肝炎病毒核心抗体(抗-HBc),通过对检测结果的观察来分析溶血对检测结果的干扰程度.结果当溶血程度大于或等于10 g/L时,ELISA一步法40%以上的标本OD值均大于临界值,差异有统计学意义(P<0.01);ELISA二步法仅为3%,差异无统计学意义(P>0.05).结论当溶血程度大于或等于10 g/L对ELISA一步法检测HBVM的结果有干扰,可造成临界假阳性;ELISA二步法干扰不明显,无统计学意义.
목적 통과실험관찰용혈표본재매련면역흡부시험(ELISA)일보법화이보법중대을형간염량대반(HBVM)검측결과적영향.방법 용80례건강인(HBVM균위음성)적혈액표본,인위조성불동정도용혈,용ELISA일보법화이보법동시검측을형간염병독표면항원(HBsAg)、을형간염병독표면항체(항-HBs)、을형간염병독e항원(HBeAg)、을형간염병독e항체(항-HBe)、을형간염병독핵심항체(항-HBc),통과대검측결과적관찰래분석용혈대검측결과적간우정도.결과 당용혈정도대우혹등우10 g/L시,ELISA일보법40%이상적표본OD치균대우림계치,차이유통계학의의(P<0.01);ELISA이보법부위3%,차이무통계학의의(P>0.05).결론 당용혈정도대우혹등우10 g/L대ELISA일보법검측HBVM적결과유간우,가조성림계가양성;ELISA이보법간우불명현,무통계학의의.
Objective To observe the effect of the hemolysis specimens in ELISA on HBVM by one-step and two-step method.Methods Collect 80 blood specimens from 80 healthy persons(negativeHBVM),and have them dissolved in different level,and then use ELISA one-step method and tow-step method to test HBsAg, anti HBs, HBeAg, anti HBe,anti HBc, and then ,analyze how the hemolysis affects the test effect.Results When the hemolysis is above 10g/L,the OD in more than 40% specimens of ELISA by one-step method is all greater than that of Cutoff(P<0.001),but by the two-step method,only 3%(P>0.05).Conclusion When the hemolysis is more than 10g/L,it has some effect on HBVM by one-step method,and it can cause critical phony positive,but it has no effect on HBVM by two-step method,so it has no meaning in statistics.