CAJ | 학술논문

对分离自健康黄鳝肠道118株菌进行点种法病原菌拮抗试验,结合产酶能力分析试验结果,得到了对病原菌拮抗作用显著和产酶能力较强的拟选菌株HY-136.通过对该菌株进行形态观察,常规生理生化鉴定和应用API 50 CHB鉴定盒鉴定,结果表明该菌与枯草芽孢杆菌属的形态及生理生化特征相似;进一步利用PCR技术对该菌株16S rRNA序列作测定与分析,序列结果在GenBank中进行Blast同源序列检索,并用Mage4.0软件与芽孢杆菌属的其它菌种进行序列分析比对,结果表明其与已报道的枯草芽孢杆菌序列(登录号为EU346662)具有99.8%的同源性,且二者在所构建的系统发育树上处于同一个分支.确定菌株HY-136为枯草芽孢杆菌(Bacillus subtilis).
대분리자건강황선장도118주균진행점충법병원균길항시험,결합산매능력분석시험결과,득도료대병원균길항작용현저화산매능력교강적의선균주HY-136.통과대해균주진행형태관찰,상규생리생화감정화응용API 50 CHB감정합감정,결과표명해균여고초아포간균속적형태급생리생화특정상사;진일보이용PCR기술대해균주16S rRNA서렬작측정여분석,서렬결과재GenBank중진행Blast동원서렬검색,병용Mage4.0연건여아포간균속적기타균충진행서렬분석비대,결과표명기여이보도적고초아포간균서렬(등록호위EU346662)구유99.8%적동원성,차이자재소구건적계통발육수상처우동일개분지.학정균주HY-136위고초아포간균(Bacillus subtilis).
One hundred and eighteen bacterial strains isolated from healthy ricefield eel(Monopterus albus)intestine were used for screening probiotics.The results of antagonistic disease bacteria by dot inoculating and the amount of extra-cellular amylase produced showed that HY-136 could strongly inhibit pathogenic bacteriums and produced more amylase.According to its morphological,physiological and biochemical characteristics,and the results detected by API 50 CHB system,we found that some characteristics of strain HY-136 were similar to Bacillus subtilis.Sequence analysis of 16S rRNA showed that strain HY-136 shared 99.8%homology with published sequence of Bacillus subtilis(accession number:EU346662).In the phylogenetic tree,both of them were on the same branch.which showed that strain HY-136 was closely related with Bacillus subtilis.Therefore,strain HY-136 was identified as Bacillus subtilis.