CAJ | 학술논문

利用电感耦合等离子质谱(ICP-MS)测定富硒双歧杆菌菌粉及其胶囊制品中硒的含量,研究富硒双歧杆菌细胞壁的破壁方法并利用高效液相色谱-电感耦合等离子质谱(HPLC-ICP-MS)对富硒双歧杆菌菌粉中硒的形态进行研究.结果表明,富硒双歧杆菌菌粉及其胶囊制品中硒的含量分别为(168.11±2.26)μg/g和(15.68±0.07)μg/g.与市售富硒产品相比较,富硒双歧杆菌胶囊中硒的含量是富硒大米的100倍,但不及富硒酵母产品中硒含量的1/10.富硒双歧杆菌产品中硒形态非常复杂,且含有许多不明硒化物,但其主要含硒氨基酸为硒代蛋氨酸,占总硒含最的(59.55±0.13)%.
이용전감우합등리자질보(ICP-MS)측정부서쌍기간균균분급기효낭제품중서적함량,연구부서쌍기간균세포벽적파벽방법병이용고효액상색보-전감우합등리자질보(HPLC-ICP-MS)대부서쌍기간균균분중서적형태진행연구.결과표명,부서쌍기간균균분급기효낭제품중서적함량분별위(168.11±2.26)μg/g화(15.68±0.07)μg/g.여시수부서산품상비교,부서쌍기간균효낭중서적함량시부서대미적100배,단불급부서효모산품중서함량적1/10.부서쌍기간균산품중서형태비상복잡,차함유허다불명서화물,단기주요함서안기산위서대단안산,점총서함최적(59.55±0.13)%.
Selenium contents and speciations in powder and capsules containing selenium-enriched Bifidobacterium subjected to cell wall disruption by combinatorial ultrasonic and enzymatic hydrolysis method were determined by high performance liquid chromatography connected with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Results indicated that the selenium contents in powder and capsules containing selenium-enriched Bifidobacterium were (168.11 ± 2.26) μg/g and (15.68 ± 0.07) μg/g, respectively. The selenium content in capsules containing selenium-enriched Bifidobacterium was nearly 100 times as high as that of commercially available selenium-enriched rice, but was less than one tenth of that of commercially available selenium-enriched yeast. A complicated selenium speciation was found in selenium-enriched Bifidobacterium products, and the major amino acid containing selenium was selenomethionine (SeMet), which was up to 59% of total selenium amount.