CAJ | 학술논문

选取大豆品种中黄18为试验材料,利用60对SSR核心引物对经过不同老化时间处理的群体及其繁殖后代群体进行遗传完整性分析.经过老化处理的群体及其繁殖子代群体与未经老化处理的对照群体相比,等位基因频率、有效等位基因数没有显著差异,结果表明经过老化处理种子及其繁殖子代群体的等位基因频率变化不大.未经老化处理、发芽率为98%的对照群体(G_0-1)与其繁殖一代、繁殖二代群体相比,等位变异数、遗传多样性指数、香农指数和稀有等位基因数没有显著差异,且遗传一致度相对较高;而经过老化处理、发芽率低于85%的群体(G_0-3和G_0-4)及其后代繁殖群体与对照群体相比,等位变异数、遗传多样性指数、香农指数和稀有等位基因数显著或极显著降低,且遗传一致度相对较低.因此,种子老化较繁殖世代对大豆种质群体的遗传结构影响更大.
선취대두품충중황18위시험재료,이용60대SSR핵심인물대경과불동노화시간처리적군체급기번식후대군체진행유전완정성분석.경과노화처리적군체급기번식자대군체여미경노화처리적대조군체상비,등위기인빈솔、유효등위기인수몰유현저차이,결과표명경과노화처리충자급기번식자대군체적등위기인빈솔변화불대.미경노화처리、발아솔위98%적대조군체(G_0-1)여기번식일대、번식이대군체상비,등위변이수、유전다양성지수、향농지수화희유등위기인수몰유현저차이,차유전일치도상대교고;이경과노화처리、발아솔저우85%적군체(G_0-3화G_0-4)급기후대번식군체여대조군체상비,등위변이수、유전다양성지수、향농지수화희유등위기인수현저혹겁현저강저,차유전일치도상대교저.인차,충자노화교번식세대대대두충질군체적유전결구영향경대.
In this study soybean cultivar Zhonghuang 18 was used as materials, and the genetic integrity of population with different germination percentage and their descendant population was investigated by 60 core SSR primers. The results showed that there was no significant difference in the allelic frequencies and the effective number of alleles of the aged population and their descendant population compared with the control population. It indicated that the allelic frequencies of the aged population and their descendant population changed slightly. There was no significant difference in the number of alleles, the index of genetic diversity, Shannon's information index and the number of rare alleles between the control population with the germination percentage of 98% and its first and second descendant population, and the value of genetic identity was relatively high. The number of alleles, the index of genetic diversity, Shannon's information index and the number of rare alleles of the aged population(G_0-3 and G_0-4)with the germination percentage below 85% and their first and second descendant population were significantly reduced compared with the control, and the value of genetic identity was relatively high. Therefore, seed aging maybe had a greater impact on the genetic composition of the soybean population than regeneration times.