高技术通讯（英文版）
高技術通訊（英文版）
고기술통신（영문판）
HIGH TECHNOLOGY LETTERS
2012年
1期
107-111
,共5页
recombinant human-salmon calcitonin%immunol fluorescence%FACS,osteoclast%hypercalcemia%saccharomyces cerevisiae
Recombinant human-salmon calcitonin (hsCT) was synthesized and expressed on the cell surfaces of recombinant Saccharomyces cerevisiae (yAGA2-hsCT) using the pAGA2-hsCT expression vector.Expression of recombinant hsCT was verified by flow cytometer.The resorption activity of osteoclasts was inhibited by recombinant hsCT protein which released from the cell surface of yAGA2-hsCT.Blood calcium determination in rats showed the activity ofyAGA2-hsCT following oral administration of 5 mg/kg lyophilized transformant yeast to hypercalcemia rats decreases the serum calcium from 2.82 ± 0.023 mM to 2.662 ± 0.012 mM.
