中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2012年
2期
117-120
,共4页
赖铭莹%刘梅%应方微%李志%朱小丽%魏花%赖平红
賴銘瑩%劉梅%應方微%李誌%硃小麗%魏花%賴平紅
뢰명형%류매%응방미%리지%주소려%위화%뢰평홍
维生素D3%糖尿病/实验性%角膜%脉络膜%视网膜
維生素D3%糖尿病/實驗性%角膜%脈絡膜%視網膜
유생소D3%당뇨병/실험성%각막%맥락막%시망막
Vitamine D3%Diabetes/experimental%Cornea%Choroid%Retina
背景 研究证实,维生素D3通过其生物活化形式1,25-(OH)2D3与维生素D受体结合,从而发挥抗炎、免疫调节、神经保护作用.此外,维生素D3可以增强胰岛素的敏感性及促进胰岛素的分泌,控制高血糖症,减轻角膜水肿.目的 研究维生素D3对缓解实验性糖尿病小鼠眼部结构损伤的作用. 方法 健康SPF级C57BL/6小鼠22只(体质量20～ 25 g),应用随机数字表法随机选取8只小鼠为维生素D3组,另取11只小鼠为糖尿病对照组,并取3只小鼠为正常对照组.利用腹腔注射质量分数2％链脲佐菌素(STZ)法制备实验性糖尿病模型,造模后2周维生素D3组小鼠行腹腔注射维生素D35 mg/kg,每周注射1次,共5次.各组每周进行血糖监测、直接检眼镜观察.各组每周随机摘取3只眼球,常规病理切片后于光学显微镜下观察眼球各部分结构,利用病理分析软件测量中央角膜、视盘旁1个视盘直径(DD)处脉络膜厚度及视网膜神经上皮层的厚度.结果 糖尿病对照组小鼠造模后第1周角膜开始出现水肿,角膜平均厚度为(339.14±11.13) μm,以后水肿持续但逐渐减轻(F=382.446,P=0.000).维生素D3干预后水肿情况明显缓解,角膜厚度明显变薄(P＜0.05).两组糖尿病小鼠第5周角膜上皮均开始萎缩(P＜0.05),糖尿病对照组至第7周萎缩情况更加严重,而维生素D3组小鼠至第7周萎缩已明显减轻(P=0.002).糖尿病小鼠脉络膜自第1周开始萎缩(P=0.010),并随时间延长萎缩加重(F=437.411,P=0.000).维生素D3干预后,第4周脉络膜萎缩趋势明显减缓,且用药后从第3周开始维生素D3组脉络膜厚度均大于糖尿病对照组(P＜0.05),糖尿病小鼠自第1周开始视网膜神经上皮层即可见明显的凹凸不平,轻度水肿(P=0.000),随时间延长,糖尿病造成的视网膜神经上皮层萎缩加重(F=91.859,P=0.000).维生素D3组视网膜神经上皮层萎缩情况无明显改变. 结论 维生素D3对实验性糖尿病小鼠角膜及脉络膜有保护作用,但其对已萎缩的视网膜并无明显的修复作用.
揹景 研究證實,維生素D3通過其生物活化形式1,25-(OH)2D3與維生素D受體結閤,從而髮揮抗炎、免疫調節、神經保護作用.此外,維生素D3可以增彊胰島素的敏感性及促進胰島素的分泌,控製高血糖癥,減輕角膜水腫.目的 研究維生素D3對緩解實驗性糖尿病小鼠眼部結構損傷的作用. 方法 健康SPF級C57BL/6小鼠22隻(體質量20～ 25 g),應用隨機數字錶法隨機選取8隻小鼠為維生素D3組,另取11隻小鼠為糖尿病對照組,併取3隻小鼠為正常對照組.利用腹腔註射質量分數2％鏈脲佐菌素(STZ)法製備實驗性糖尿病模型,造模後2週維生素D3組小鼠行腹腔註射維生素D35 mg/kg,每週註射1次,共5次.各組每週進行血糖鑑測、直接檢眼鏡觀察.各組每週隨機摘取3隻眼毬,常規病理切片後于光學顯微鏡下觀察眼毬各部分結構,利用病理分析軟件測量中央角膜、視盤徬1箇視盤直徑(DD)處脈絡膜厚度及視網膜神經上皮層的厚度.結果 糖尿病對照組小鼠造模後第1週角膜開始齣現水腫,角膜平均厚度為(339.14±11.13) μm,以後水腫持續但逐漸減輕(F=382.446,P=0.000).維生素D3榦預後水腫情況明顯緩解,角膜厚度明顯變薄(P＜0.05).兩組糖尿病小鼠第5週角膜上皮均開始萎縮(P＜0.05),糖尿病對照組至第7週萎縮情況更加嚴重,而維生素D3組小鼠至第7週萎縮已明顯減輕(P=0.002).糖尿病小鼠脈絡膜自第1週開始萎縮(P=0.010),併隨時間延長萎縮加重(F=437.411,P=0.000).維生素D3榦預後,第4週脈絡膜萎縮趨勢明顯減緩,且用藥後從第3週開始維生素D3組脈絡膜厚度均大于糖尿病對照組(P＜0.05),糖尿病小鼠自第1週開始視網膜神經上皮層即可見明顯的凹凸不平,輕度水腫(P=0.000),隨時間延長,糖尿病造成的視網膜神經上皮層萎縮加重(F=91.859,P=0.000).維生素D3組視網膜神經上皮層萎縮情況無明顯改變. 結論 維生素D3對實驗性糖尿病小鼠角膜及脈絡膜有保護作用,但其對已萎縮的視網膜併無明顯的脩複作用.
배경 연구증실,유생소D3통과기생물활화형식1,25-(OH)2D3여유생소D수체결합,종이발휘항염、면역조절、신경보호작용.차외,유생소D3가이증강이도소적민감성급촉진이도소적분비,공제고혈당증,감경각막수종.목적 연구유생소D3대완해실험성당뇨병소서안부결구손상적작용. 방법 건강SPF급C57BL/6소서22지(체질량20～ 25 g),응용수궤수자표법수궤선취8지소서위유생소D3조,령취11지소서위당뇨병대조조,병취3지소서위정상대조조.이용복강주사질량분수2％련뇨좌균소(STZ)법제비실험성당뇨병모형,조모후2주유생소D3조소서행복강주사유생소D35 mg/kg,매주주사1차,공5차.각조매주진행혈당감측、직접검안경관찰.각조매주수궤적취3지안구,상규병리절편후우광학현미경하관찰안구각부분결구,이용병리분석연건측량중앙각막、시반방1개시반직경(DD)처맥락막후도급시망막신경상피층적후도.결과 당뇨병대조조소서조모후제1주각막개시출현수종,각막평균후도위(339.14±11.13) μm,이후수종지속단축점감경(F=382.446,P=0.000).유생소D3간예후수종정황명현완해,각막후도명현변박(P＜0.05).량조당뇨병소서제5주각막상피균개시위축(P＜0.05),당뇨병대조조지제7주위축정황경가엄중,이유생소D3조소서지제7주위축이명현감경(P=0.002).당뇨병소서맥락막자제1주개시위축(P=0.010),병수시간연장위축가중(F=437.411,P=0.000).유생소D3간예후,제4주맥락막위축추세명현감완,차용약후종제3주개시유생소D3조맥락막후도균대우당뇨병대조조(P＜0.05),당뇨병소서자제1주개시시망막신경상피층즉가견명현적요철불평,경도수종(P=0.000),수시간연장,당뇨병조성적시망막신경상피층위축가중(F=91.859,P=0.000).유생소D3조시망막신경상피층위축정황무명현개변. 결론 유생소D3대실험성당뇨병소서각막급맥락막유보호작용,단기대이위축적시망막병무명현적수복작용.
Background Research demonstrated that vitamin D3 mediated by its receptor has the potent nonclassical effects,including immunomodulatory,antiinflammatory,and neuroprotective properties,and it can enhance the secretion and sensitivity of insulin and therefore down-regulate hyperglycemia and attenuate the corneal edema.Objective The present study was to investigate the protective effect of vitamin D3on ocular structure in experimental diabetic rat. Methods Twenty-two healthy SPF C57BL/6 rats were randomly divided into vitamine D3 group (8 rabbits),diabetic control group ( 11 rabbits) and normal control group ( 3 rabbits).2％ streptozotocin ( STZ,175 mg/kg)was intraperitoneally injected to create the diabetic models in the rats of the vitamine D3 group and diabetic control group.Blood glucose was examined for 3 times in the third day after STZ injection,and the rats with the blood glucose concentration ＞16.7 mmol/L was identified as the successful diabetic models.After modeling,the rat tail blood was collected for the monitoring of blood glucose.Two weeks after modeling,vitamine D3 was intraperitoneally injected in each week for 5 times.The fundus was examined using direct ophtalmoscope,and the eyeballs were obtained under the excessive anesthesia for the measurement of thickness of the central cornea,retina and choroids by histopathological examination once a week for 7 weeks after administration of vitamin D3.The administration of the animals complied with the Statement of ARVO. Results The corneal edema appeared with the corneal thickness of (339.14± 11.13) μm in the first week and gradually attenuated with time elapse after modeling in the diabetic group ( F =382.446,P =0.000).The corneal thickness values were significantly decreased from the second week through the seventh week in the vitamin D3 group compared with diabetic control group(P＜0.05).The atrophy of the corneal epithelium was found from the fifth week to the seventh week in diabetic control group,but that in vitamin D3 group was slight (P＜0.05).The gradually thinning of the choroids was seen from the first week to the seventh week in the diabetic control group ( F =437.411,P =0.000 ),however,the thickness values in the vitamin D3 group were significantly increased in comparison with the diabetic control group in various time points (P＜0.05).The retina thickness was gradually reduced during the seven-week duration in the diabetic control group (F =91.859,P =0.000),but no significant change was identified in retina thickness in the vitamin D3 group(P＞0.05). Conclusions Vitamin D3 has prevent and therapeutic effects on experimental diabetic oculopathy.
