中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2011年
8期
649-653
,共5页
血管内皮生长因子%多态现象,遗传学%糖尿病,2型%糖尿病周围神经病变
血管內皮生長因子%多態現象,遺傳學%糖尿病,2型%糖尿病週圍神經病變
혈관내피생장인자%다태현상,유전학%당뇨병,2형%당뇨병주위신경병변
Vascular endothelial growth factor%Polymorphism,genetics%Diabetes mellitus,type 2%Diabetic peripheral neuropathy
目的 探讨血管内皮生长因子(VEGF)基因3'-非翻译区936C/T多态性与山东地区汉族人2型糖尿病合并周围神经病变(DPN)之间的关系.方法 194例糖尿病患者分为单纯糖尿病组(n=92)和糖尿病神经病变组(n=102),另120名健康个体设为健康对照组.采用PCR-限制性片段长度多态性(RFLP)方法确定全部个体的基因型;对不同基因型间及病例组间的临床与生化参数、血清VEGF浓度以及VEGF基因936C/T多态性进行了统计分析.结果 糖尿病神经病变组C等位基因及CC基因型频率显著高于对照组(x2为9.406和9.677,P＜0.05)和糖尿病组(x2为5.578和5.614,P＜0.05),而携带T等位基因的基因型(CT+TT)频率及T等位基因频率显著低于对照组(x2为9.406和9.677,P＜0.05)和糖尿病组(x2为5.578和5.614,P＜0.05).Logistic多元回归分析显示血清低密度脂蛋白胆固醇(LDL-C)、总胆固醇、HbA1c水平以及VEGF浓度与DPN发生呈正相关,而VEGF基因936C/T多态性与糖尿病周围神经病变发病危险呈负相关(β=-1.046,OR=0.457,P=0.006,95%CI:0.166～0.741).结论 中国山东地区汉族人群中存在VEGF基因936C/T多态性,C等位基因及CC基因型患者可能是糖尿病易于发生神经病变危险性的遗传标志,而T等位基因和携带T等位基因的基因型(936TF基因型和936CT基因型)可能是降低糖尿病发生神经病变风险的遗传标志.
目的 探討血管內皮生長因子(VEGF)基因3'-非翻譯區936C/T多態性與山東地區漢族人2型糖尿病閤併週圍神經病變(DPN)之間的關繫.方法 194例糖尿病患者分為單純糖尿病組(n=92)和糖尿病神經病變組(n=102),另120名健康箇體設為健康對照組.採用PCR-限製性片段長度多態性(RFLP)方法確定全部箇體的基因型;對不同基因型間及病例組間的臨床與生化參數、血清VEGF濃度以及VEGF基因936C/T多態性進行瞭統計分析.結果 糖尿病神經病變組C等位基因及CC基因型頻率顯著高于對照組(x2為9.406和9.677,P＜0.05)和糖尿病組(x2為5.578和5.614,P＜0.05),而攜帶T等位基因的基因型(CT+TT)頻率及T等位基因頻率顯著低于對照組(x2為9.406和9.677,P＜0.05)和糖尿病組(x2為5.578和5.614,P＜0.05).Logistic多元迴歸分析顯示血清低密度脂蛋白膽固醇(LDL-C)、總膽固醇、HbA1c水平以及VEGF濃度與DPN髮生呈正相關,而VEGF基因936C/T多態性與糖尿病週圍神經病變髮病危險呈負相關(β=-1.046,OR=0.457,P=0.006,95%CI:0.166～0.741).結論 中國山東地區漢族人群中存在VEGF基因936C/T多態性,C等位基因及CC基因型患者可能是糖尿病易于髮生神經病變危險性的遺傳標誌,而T等位基因和攜帶T等位基因的基因型(936TF基因型和936CT基因型)可能是降低糖尿病髮生神經病變風險的遺傳標誌.
목적 탐토혈관내피생장인자(VEGF)기인3'-비번역구936C/T다태성여산동지구한족인2형당뇨병합병주위신경병변(DPN)지간적관계.방법 194례당뇨병환자분위단순당뇨병조(n=92)화당뇨병신경병변조(n=102),령120명건강개체설위건강대조조.채용PCR-한제성편단장도다태성(RFLP)방법학정전부개체적기인형;대불동기인형간급병례조간적림상여생화삼수、혈청VEGF농도이급VEGF기인936C/T다태성진행료통계분석.결과 당뇨병신경병변조C등위기인급CC기인형빈솔현저고우대조조(x2위9.406화9.677,P＜0.05)화당뇨병조(x2위5.578화5.614,P＜0.05),이휴대T등위기인적기인형(CT+TT)빈솔급T등위기인빈솔현저저우대조조(x2위9.406화9.677,P＜0.05)화당뇨병조(x2위5.578화5.614,P＜0.05).Logistic다원회귀분석현시혈청저밀도지단백담고순(LDL-C)、총담고순、HbA1c수평이급VEGF농도여DPN발생정정상관,이VEGF기인936C/T다태성여당뇨병주위신경병변발병위험정부상관(β=-1.046,OR=0.457,P=0.006,95%CI:0.166～0.741).결론 중국산동지구한족인군중존재VEGF기인936C/T다태성,C등위기인급CC기인형환자가능시당뇨병역우발생신경병변위험성적유전표지,이T등위기인화휴대T등위기인적기인형(936TF기인형화936CT기인형)가능시강저당뇨병발생신경병변풍험적유전표지.
Objective To elucidate the relationship between a 936C/T mutation at 3'-untranslated region of human vascular endothelial growth factor(VEGF) gene and diabetic peripheral neuropathy ( DPN ). Methods All subjects recruited in this study were assigned into DM (n = 92, diabetes without neuropathy, retinopathy or nephropathy), DPN (n = 102, diabetes with peripheral neuropathy only ), and healthy control (n = 120 ) groups,respectively. The gene polymorphism was determined by PCR-RFLP, as well as the other clinical parameters including serum VEGF by ELISA. Results The frequencies of both genotype CC and allele C were significantly higher in DPN group than those in either DM group(x2 = 5.578 and 5.614, P＜0. 05 ) or control group (x2 = 9. 406 and 9. 677, P＜0. 05 ). However, the frequencies of genotype(CT+TT) and allele T were significantly lower in DPN group than that in either DM group(x2 =5.578 and 5.614, P＜0. 05) and control group (x2=9.406 and 9.677, P＜0.05). The multivariate logistic regression analysis showed that the levels of HbA1c, total cholesterol, low-density lipoproteincholesterol( LDL-C ), and serum VEGF positively correlated with DPN, while the 936C/T polymorphism of VEGF gene negatively correlated with DPN(β= -1. 046, OR=0. 457, P=0. 006, 95% CI: 0. 166-0. 741 ). Conclusions Allele 936C of VEGF gene may serve as a genetic marker susceptible to DPN, while allele 936T may be a protective genetic marker of DPN.
