中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
1期
91-94
,共4页
江楠%肖亮灿%张劲军%邓晟华%黄文起
江楠%肖亮燦%張勁軍%鄧晟華%黃文起
강남%초량찬%장경군%산성화%황문기
低温,人工%再灌注损伤%脑%缺氧诱导因子-1α亚基%葡萄糖转运体1型
低溫,人工%再灌註損傷%腦%缺氧誘導因子-1α亞基%葡萄糖轉運體1型
저온,인공%재관주손상%뇌%결양유도인자-1α아기%포도당전운체1형
Hypothermia,induced%Reperfusion injury%Brain%Hypoxia-inducible factor 1,alpha subunit%Glucose transporter type 1
目的 探讨亚低温对大鼠慢性脑缺血再灌注时缺氧诱导因子-1α(HIF-1α)和葡萄糖转运蛋白-1(GLUT-1)表达的影响.方法 健康清洁级雌性SD大鼠36只,体重170～210 g,采用随机数字表法,将大鼠随机分为3组(n=12):假手术组(S组)、常温再灌注组(NT组)和亚低温再灌注组(MH组).除S组外,其余2组采用右侧颈总动脉与右侧颈外静脉端端吻合6周恢复灌注的方法制备大鼠慢性脑缺血再灌注模型.再灌注期间S组和NT组维持直肠温37～38 ℃,MH组维持直肠温31.5～32.5℃.分别于再灌注3和48 h时每组处死6只大鼠,取脑组织,采用免疫组化法和荧光PCR法测定HIF-1α和GLUT-1及其mRNA的表达水平,电镜下观察血脑屏障的超微结构.结果 与S组比较,NT组再灌注3和48 h时脑组织HIF-1α及其mRNA表达上调,再灌注48 h时GLUT-1及其mRNA表达下调,再灌注3 h时GLUT-1 mRNA表达上调(P＜0.05);与NT组比较,MH组再灌注48 h时HIF-1α及其mRNA表达下调,再灌注3 h时HIF-1α mRNA表达下调,再灌注48 h时GLUT-1及其mRNA表达上调,再灌注3 h时GLUT-1 mRNA表达下调(P＜0.05).MH组血脑屏障结构损伤程度轻于NT组.结论 亚低温可下调HIF-1α表达,上调GLUT-1表达,从而减轻大鼠慢性脑缺血再灌注损伤.
目的 探討亞低溫對大鼠慢性腦缺血再灌註時缺氧誘導因子-1α(HIF-1α)和葡萄糖轉運蛋白-1(GLUT-1)錶達的影響.方法 健康清潔級雌性SD大鼠36隻,體重170～210 g,採用隨機數字錶法,將大鼠隨機分為3組(n=12):假手術組(S組)、常溫再灌註組(NT組)和亞低溫再灌註組(MH組).除S組外,其餘2組採用右側頸總動脈與右側頸外靜脈耑耑吻閤6週恢複灌註的方法製備大鼠慢性腦缺血再灌註模型.再灌註期間S組和NT組維持直腸溫37～38 ℃,MH組維持直腸溫31.5～32.5℃.分彆于再灌註3和48 h時每組處死6隻大鼠,取腦組織,採用免疫組化法和熒光PCR法測定HIF-1α和GLUT-1及其mRNA的錶達水平,電鏡下觀察血腦屏障的超微結構.結果 與S組比較,NT組再灌註3和48 h時腦組織HIF-1α及其mRNA錶達上調,再灌註48 h時GLUT-1及其mRNA錶達下調,再灌註3 h時GLUT-1 mRNA錶達上調(P＜0.05);與NT組比較,MH組再灌註48 h時HIF-1α及其mRNA錶達下調,再灌註3 h時HIF-1α mRNA錶達下調,再灌註48 h時GLUT-1及其mRNA錶達上調,再灌註3 h時GLUT-1 mRNA錶達下調(P＜0.05).MH組血腦屏障結構損傷程度輕于NT組.結論 亞低溫可下調HIF-1α錶達,上調GLUT-1錶達,從而減輕大鼠慢性腦缺血再灌註損傷.
목적 탐토아저온대대서만성뇌결혈재관주시결양유도인자-1α(HIF-1α)화포도당전운단백-1(GLUT-1)표체적영향.방법 건강청길급자성SD대서36지,체중170～210 g,채용수궤수자표법,장대서수궤분위3조(n=12):가수술조(S조)、상온재관주조(NT조)화아저온재관주조(MH조).제S조외,기여2조채용우측경총동맥여우측경외정맥단단문합6주회복관주적방법제비대서만성뇌결혈재관주모형.재관주기간S조화NT조유지직장온37～38 ℃,MH조유지직장온31.5～32.5℃.분별우재관주3화48 h시매조처사6지대서,취뇌조직,채용면역조화법화형광PCR법측정HIF-1α화GLUT-1급기mRNA적표체수평,전경하관찰혈뇌병장적초미결구.결과 여S조비교,NT조재관주3화48 h시뇌조직HIF-1α급기mRNA표체상조,재관주48 h시GLUT-1급기mRNA표체하조,재관주3 h시GLUT-1 mRNA표체상조(P＜0.05);여NT조비교,MH조재관주48 h시HIF-1α급기mRNA표체하조,재관주3 h시HIF-1α mRNA표체하조,재관주48 h시GLUT-1급기mRNA표체상조,재관주3 h시GLUT-1 mRNA표체하조(P＜0.05).MH조혈뇌병장결구손상정도경우NT조.결론 아저온가하조HIF-1α표체,상조GLUT-1표체,종이감경대서만성뇌결혈재관주손상.
Objective To investigate the effect of mild hypothermia on the expression of hypoxia-inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in a rat model of chronic cerebral ischemia-reperfusion.Methods Thirty-six female SD rats weighing 170-210 g were randomly divided into 3 groups (n = 12 each):sham operation group (group S), normal body temperature group (group NT ) and mild hypothermia group (group MH). Arterio-venous fistula was established by end-to-end anastomosis between the right common carotid artery and right external jugular vein for 6 weeks followed by reperfusion. In group MH, mild hypothermia was induced at the initiation of reperfusion and the rectal temperature was reduced to 31.5-32.5 ℃. In group S and NT, the rectal temperature was maintained at 37-38 ℃. Six rats in each group were sacrificed at 3 and 48 h of reperfusion. The brains were immediately removed for determination of the expression of HIF-1α, GLUT-1, HIF-1α mRNA and GLUT-1 mRNA and microscopic examination. Results Compared with group S, the expression of HIF-1α and HIF-1α mRNA at 3 and 48 h of reperfusion and GLUT-1 mRNA at 3 h of reperfusion was up-regulated, while the expression of GLUT-1 and GLUT-1 mRNA at 48 h of reperfusion was down-regulated in group NT (P ＜ 0.05).Compared with group NT, the expression of HIF-1α and HIF-1α mRNA at 48 h of reperfusion and HIF-1α mRNA and GLUT-1 mRNA at 3 h of reperfusion was down-regulated, while the expression of GLUT-1 and GLUT-1 mRNA at 48 h of reperfusion was up-regulated in group MH (P ＜ 0.05).Microscopic examination showed that the injury to the ultrastructure of blood-brain barrier was significantly attenuated in group MH compared with group NT. Conclusion Mild hypothermia can attenuate chronic ischemia-reperfusion injury by down-regulating the expression of HIF-1α and up-regulating the expression of GLUT-1.
