中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
5期
513-516
,共4页
唐琨%左双燕%李颖%郑元春%霍秋波%于季红%张圆%倪雪冰%姚囡囡%谭红专
唐琨%左雙燕%李穎%鄭元春%霍鞦波%于季紅%張圓%倪雪冰%姚囡囡%譚紅專
당곤%좌쌍연%리영%정원춘%곽추파%우계홍%장원%예설빙%요닙닙%담홍전
伯氏疏螺旋体%斑点热群立克次体%复合感染%序列分析
伯氏疏螺鏇體%斑點熱群立剋次體%複閤感染%序列分析
백씨소라선체%반점열군립극차체%복합감염%서렬분석
Borrelia burgdorferi sensu lato%Spotted fever group Rickettsiae%Co-infection%Sequence analysis
目的 了解黑龙江省旅游区蜱伯氏疏螺旋体和斑点热群立克次体复合感染的动态变化.方法 运用PCR方法检测2010年捕获蜱中两种病原感染状况并测序进行序列分析.结果 在威虎山和镜泊湖景区采集点捕获蜱标本共849只.两地优势蜱种分别为全沟硬蜱和嗜群血蜱,其中伯氏疏螺旋体和斑点热群立克次体感染率分别为26.15％、10.05％和0.00％、13.33％,提示全沟硬蜱复合感染.两地优势蜱种活动高峰分别出现在7月和6月上旬,蜱携带病原率因时间差异而不同.序列分析显示旅游区伯氏疏螺旋体有3种基因型,除B.garinii型、B.afzelii型外,首次在东北地区发现B.valaisiana-like group型.对斑点热群立克次体序列分析表明,所得两序列分别与Rickettsia sp.HL-93和Rickettsia sp.H820,其同源性为100％.结论 两旅游区蜱中存在伯氏疏螺旋体和斑点热群立克次体复合感染,且携带的基因类型存在多样性；动态调查显示蜱种、蜱密度的分布以及蜱携带病原状况因生境、时间和人群的不同而有差异.
目的 瞭解黑龍江省旅遊區蜱伯氏疏螺鏇體和斑點熱群立剋次體複閤感染的動態變化.方法 運用PCR方法檢測2010年捕穫蜱中兩種病原感染狀況併測序進行序列分析.結果 在威虎山和鏡泊湖景區採集點捕穫蜱標本共849隻.兩地優勢蜱種分彆為全溝硬蜱和嗜群血蜱,其中伯氏疏螺鏇體和斑點熱群立剋次體感染率分彆為26.15％、10.05％和0.00％、13.33％,提示全溝硬蜱複閤感染.兩地優勢蜱種活動高峰分彆齣現在7月和6月上旬,蜱攜帶病原率因時間差異而不同.序列分析顯示旅遊區伯氏疏螺鏇體有3種基因型,除B.garinii型、B.afzelii型外,首次在東北地區髮現B.valaisiana-like group型.對斑點熱群立剋次體序列分析錶明,所得兩序列分彆與Rickettsia sp.HL-93和Rickettsia sp.H820,其同源性為100％.結論 兩旅遊區蜱中存在伯氏疏螺鏇體和斑點熱群立剋次體複閤感染,且攜帶的基因類型存在多樣性；動態調查顯示蜱種、蜱密度的分佈以及蜱攜帶病原狀況因生境、時間和人群的不同而有差異.
목적 료해흑룡강성여유구비백씨소라선체화반점열군립극차체복합감염적동태변화.방법 운용PCR방법검측2010년포획비중량충병원감염상황병측서진행서렬분석.결과 재위호산화경박호경구채집점포획비표본공849지.량지우세비충분별위전구경비화기군혈비,기중백씨소라선체화반점열군립극차체감염솔분별위26.15％、10.05％화0.00％、13.33％,제시전구경비복합감염.량지우세비충활동고봉분별출현재7월화6월상순,비휴대병원솔인시간차이이불동.서렬분석현시여유구백씨소라선체유3충기인형,제B.garinii형、B.afzelii형외,수차재동북지구발현B.valaisiana-like group형.대반점열군립극차체서렬분석표명,소득량서렬분별여Rickettsia sp.HL-93화Rickettsia sp.H820,기동원성위100％.결론 량여유구비중존재백씨소라선체화반점열군립극차체복합감염,차휴대적기인류형존재다양성；동태조사현시비충、비밀도적분포이급비휴대병원상황인생경、시간화인군적불동이유차이.
Objective To monitor the co-infection status of Borrelia burgdorferi sensu lato (R.b.s.1) and spotted fever group Rickettsia (SFGR) in tourist areas of Heilongjiang province.Methods Polymerase chain reaction (PCR) was used to detect the 5S-23S rRNA intergenic spacer of B.b.s.1 and ompA of SFGR in ticks,dynamically collected from tourist areas of Heilongjiang province in 2010.Amplification products from positive ticks were sequenced,and phylogenetic analysis was conducted by Mega 5.0 software package.Results 849 ticks were collected from two tourist points,with the dominant ticks in Tiger Mountain and Jingpo Lake were Ixodes persulcatus and Haemaphysalis concinna.Regarding the Ixodes persulcatus from Tiger Mountain,the infection rates of B.b.s.1 and SFGR were 26.15％ and 10.05％.The infection rate of SFGR was 13.33％ in Haemaphysalis concinna and the B.b.s.1 was tndiscovered in the same ticks from Jingpo Lake.However,the co-infection could only be detected in Ixodes persulcatus of both tourist areas.Surveillance data showed that the major ticks were more likely to be appeared in July at Tiger Mountain and in June at Jingpo Lake.Data from the sequence analysis on B.b.s.1 showed that the B.b.s.1 in tourist areas could be classified into three different genotypes,other than B.garinii and B.afzelii.We first detected B.valaisiana-like group genotype in northeast of China.Results from the sequence analysis of SFGR positive products showed that the two DNA sequences of newly detected agents were completely the same as Rickettsia sp.HL-93 which was detected in Hulin and Rickettsia sp.H820 found in northeast,China.Conclusion The co-infection of B.b.s.1 and SFGR was detected in ticks from the tourist areas of Heilongjiang province,and data from the sequencing of specific fragment showed that various kinds of genotypes existed in this area.However; the rates of co-infectionitis-different according to environment,time and population that contributed to the kinds of and the index of ticks existed in the surveys points,also the infection rate of the ticks was studied.
