中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2009年
1期
33-36
,共4页
顾志峰%金鸥阳%徐婷%马晓蕾%赵盛楠%曹晓蕾%孙凌云
顧誌峰%金鷗暘%徐婷%馬曉蕾%趙盛楠%曹曉蕾%孫凌雲
고지봉%금구양%서정%마효뢰%조성남%조효뢰%손릉운
间质干细胞%肺疾病%间质性%红斑狼疮%系统性
間質榦細胞%肺疾病%間質性%紅斑狼瘡%繫統性
간질간세포%폐질병%간질성%홍반랑창%계통성
Mesenchymal stem cells%Lung disease,interstitial%Lupus erythematosus,systemic
目的 探讨脐带间质干细胞(UC-MSCs)对MRL/lpr狼疮鼠间质性肺炎的治疗作用.方法 体外分离培养UC-MSCs,将24只18周龄雌性MRL/lpr小鼠采用随机数字表法分为1次治疗组、3次治疗组和对照组,1次治疗组和3次治疗组于第18周给予1×106第3代UC-MSCs尾静脉注射,3次治疗组在第19、20周时再分别重复1次;对照组给予0.5 ml生理盐水尾静脉注射.在第29周时处死小鼠,用HE染色观察各组小鼠的肺病理变化.结果 UC-MSCs移植能显著减轻MRL/lpr狼疮鼠的间质性肺炎.1次治疗组和3次治疗组的肺气管损害指数分别为1.40±0.24和1.02±0.29,明显低于对照组的1.95±0.35,差异有统计学意义(q值分别为0.551和0.937,均P<0.01);1次治疗组和3次治疗组的血管损害指数分别为1.20±0.18和1.08±0.16,明显低于对照组的1.56±0.32,差异有统计学意义(q值分别为0.360和0.479,P<0.05和P<0.01);1次治疗组和3次治疗组的肺间质中炎症细胞浸润程度分别为1.30±0.21和1.05±0.15,明显低于对照组的1.72±0.34,差异有统计学意义(q值分别为0.417和0.673,P<0.05和P<0.01).结论 UC-MSCs移植对MRL/lpr狼疮鼠的间质性肺炎具有治疗作用.
目的 探討臍帶間質榦細胞(UC-MSCs)對MRL/lpr狼瘡鼠間質性肺炎的治療作用.方法 體外分離培養UC-MSCs,將24隻18週齡雌性MRL/lpr小鼠採用隨機數字錶法分為1次治療組、3次治療組和對照組,1次治療組和3次治療組于第18週給予1×106第3代UC-MSCs尾靜脈註射,3次治療組在第19、20週時再分彆重複1次;對照組給予0.5 ml生理鹽水尾靜脈註射.在第29週時處死小鼠,用HE染色觀察各組小鼠的肺病理變化.結果 UC-MSCs移植能顯著減輕MRL/lpr狼瘡鼠的間質性肺炎.1次治療組和3次治療組的肺氣管損害指數分彆為1.40±0.24和1.02±0.29,明顯低于對照組的1.95±0.35,差異有統計學意義(q值分彆為0.551和0.937,均P<0.01);1次治療組和3次治療組的血管損害指數分彆為1.20±0.18和1.08±0.16,明顯低于對照組的1.56±0.32,差異有統計學意義(q值分彆為0.360和0.479,P<0.05和P<0.01);1次治療組和3次治療組的肺間質中炎癥細胞浸潤程度分彆為1.30±0.21和1.05±0.15,明顯低于對照組的1.72±0.34,差異有統計學意義(q值分彆為0.417和0.673,P<0.05和P<0.01).結論 UC-MSCs移植對MRL/lpr狼瘡鼠的間質性肺炎具有治療作用.
목적 탐토제대간질간세포(UC-MSCs)대MRL/lpr랑창서간질성폐염적치료작용.방법 체외분리배양UC-MSCs,장24지18주령자성MRL/lpr소서채용수궤수자표법분위1차치료조、3차치료조화대조조,1차치료조화3차치료조우제18주급여1×106제3대UC-MSCs미정맥주사,3차치료조재제19、20주시재분별중복1차;대조조급여0.5 ml생리염수미정맥주사.재제29주시처사소서,용HE염색관찰각조소서적폐병리변화.결과 UC-MSCs이식능현저감경MRL/lpr랑창서적간질성폐염.1차치료조화3차치료조적폐기관손해지수분별위1.40±0.24화1.02±0.29,명현저우대조조적1.95±0.35,차이유통계학의의(q치분별위0.551화0.937,균P<0.01);1차치료조화3차치료조적혈관손해지수분별위1.20±0.18화1.08±0.16,명현저우대조조적1.56±0.32,차이유통계학의의(q치분별위0.360화0.479,P<0.05화P<0.01);1차치료조화3차치료조적폐간질중염증세포침윤정도분별위1.30±0.21화1.05±0.15,명현저우대조조적1.72±0.34,차이유통계학의의(q치분별위0.417화0.673,P<0.05화P<0.01).결론 UC-MSCs이식대MRL/lpr랑창서적간질성폐염구유치료작용.
Objective To investigate the effects of umbilical cord mesenchymal stem cell (UC-MSCs) transplantation on interstitial pneumonitis in MRL/lpr mice. Methods Twenty four 18-week-old MRL/lpr female mice were randomly divided into 3 groups: the single dose group received a single dose of UC-MSCs (1×10<'6>) transfusion intravenously, the 3 dose group received 3 injections of UC-MSCs (1×10<'6>) weekly for 3 weeks, and the control mice were treated with saline. Both the control and the treated mice were sacrificed at 29 weeks. The histopathology of the lungs were assessed by HE staining. Results In comparison to the control mice, UC-MSCs transplantation significantly attenuated interstitial pneumonitis in the MRL/lpr mice. The peribronchiolar lesion indices of the single dose treatment group (1.40±0.24) and the 3 dose treatment group (1.02±0.29) were significantly decreased as compared to the control group (1.95±0.35), q=0.551,0.937, all P<0.01. The perivascular lesion index of the single dose treatment group (1.20±0.18) and the 3 dose treatment group (1.08±0.16) were also significantly reduced as compared to the control group (1.56±0.32), q =0.360, 0.479, P<0.05, P<0.01. The inflammatory cell infiltration index of the control group (1.72±0.34) was significantly increased compared to the single dose treatment group (1.30±0.21) and the 3 dose treatment group (1.05±0.15), q=0.417,0.673, P<0.05, P<0.01. Conclusions These findings indicate that UC-MSCs have a pleiotropic therapeutic effect on pneumonitis in MRL/lpr mice.
