中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
5期
355-357
,共3页
疱疹病毒4型,人%传染性单核细胞增多症%受体,CCR3%受体,CCR5%T淋巴细胞亚群
皰疹病毒4型,人%傳染性單覈細胞增多癥%受體,CCR3%受體,CCR5%T淋巴細胞亞群
포진병독4형,인%전염성단핵세포증다증%수체,CCR3%수체,CCR5%T림파세포아군
Herpesvirus 4,human%Infectious mononucleosis%Receptors,CCR3%Receptors,CCR5%T-Lymphocyte subsets
目的 探讨感染EB病毒的传染性单核细胞增多症(IM)患儿CCR3和CCR5的表达,以期了解Th1/Th2细胞的分化情况.方法 观察患儿外周血异型淋巴细胞比例,测定患儿的嗜异凝集抗体,用双抗体夹心酶联免疫吸附法(ELISA)测定患儿抗EBV-CA-IgM、抗EBV-CA-IgG及抗EBV-NA-IgG,筛选出符合诊断标准的IM患儿.并用流式细胞检测仪检测淋巴细胞中CCR3和CCR5的表达.结果 IM感染组异型淋巴细胞比例高于对照组(P＜0.05).IM感染组CCR3+细胞率高于对照组(P＜0.05),CCR5+细胞率低于对照组(P＜0.05).CCR3与发热持续时间、异型淋巴细胞百分数呈正相关(P＜0.05).CCR5与发热持续时间呈负相关(P＜0.05).结论 IM患儿CCR3+表达增高,CCR5+表达降低,存在以Th2细胞优势分化状态为特征的T辅助细胞分化失衡.CCR3和CCR5可以作为判断IM病情轻重的重要参考指标.
目的 探討感染EB病毒的傳染性單覈細胞增多癥(IM)患兒CCR3和CCR5的錶達,以期瞭解Th1/Th2細胞的分化情況.方法 觀察患兒外週血異型淋巴細胞比例,測定患兒的嗜異凝集抗體,用雙抗體夾心酶聯免疫吸附法(ELISA)測定患兒抗EBV-CA-IgM、抗EBV-CA-IgG及抗EBV-NA-IgG,篩選齣符閤診斷標準的IM患兒.併用流式細胞檢測儀檢測淋巴細胞中CCR3和CCR5的錶達.結果 IM感染組異型淋巴細胞比例高于對照組(P＜0.05).IM感染組CCR3+細胞率高于對照組(P＜0.05),CCR5+細胞率低于對照組(P＜0.05).CCR3與髮熱持續時間、異型淋巴細胞百分數呈正相關(P＜0.05).CCR5與髮熱持續時間呈負相關(P＜0.05).結論 IM患兒CCR3+錶達增高,CCR5+錶達降低,存在以Th2細胞優勢分化狀態為特徵的T輔助細胞分化失衡.CCR3和CCR5可以作為判斷IM病情輕重的重要參攷指標.
목적 탐토감염EB병독적전염성단핵세포증다증(IM)환인CCR3화CCR5적표체,이기료해Th1/Th2세포적분화정황.방법 관찰환인외주혈이형림파세포비례,측정환인적기이응집항체,용쌍항체협심매련면역흡부법(ELISA)측정환인항EBV-CA-IgM、항EBV-CA-IgG급항EBV-NA-IgG,사선출부합진단표준적IM환인.병용류식세포검측의검측림파세포중CCR3화CCR5적표체.결과 IM감염조이형림파세포비례고우대조조(P＜0.05).IM감염조CCR3+세포솔고우대조조(P＜0.05),CCR5+세포솔저우대조조(P＜0.05).CCR3여발열지속시간、이형림파세포백분수정정상관(P＜0.05).CCR5여발열지속시간정부상관(P＜0.05).결론 IM환인CCR3+표체증고,CCR5+표체강저,존재이Th2세포우세분화상태위특정적T보조세포분화실형.CCR3화CCR5가이작위판단IM병정경중적중요삼고지표.
Objective To explore the expression of periphery blood leucocyte CCR3 and CCR5 and to comprehend T helper cell in the Children with Epstein-Barr virus associated infectious mononucleosis.Methods We defined the children according to the diagnosis criterion through Paul-Bunnell test inspecting the children's periphery blood unusual lymphocyte and detecting their anti-EBV-CA-IgM, anti-EBV-CA-IgG and anti-EBV-NA-IgG by ELISA and counted the ratio of CCR3 + and CCR5 + cells in lymphocytes with flow cytometry. Results The ratio of unusual lymphocyte in IM was higher than that of the healthy control group (P ＜ O. 05). The ratio of CCR3 + cells in IM group was higher than that of the healthy control group (P ＜ 0.05). The ratio of CCR5 + cells in IM group was significantly lower than that of the healthy control group. CCR3 + had direct interrelation with fever continued time and the ratio of unusual lymphocyte. There was a negative interrelation between CCR5 and fever continued time ( P ＜ 0. 05 ). Conclusions Children infectious of IM expressed higher level of CCR3 + and lower level of CCR5 + and there was a tendency of Th2 polarization with over production of T helper cell divide imbalance. CCR3 + and CCR5 + may be important targets to judge the degree of seriousness of IM.
