CAJ | 학술논문

目的研究晚期糖基化终产物(AGEs)对人神经母细胞瘤SH-SY5Y细胞株增殖、凋亡以及AD相关mRNA的影响。方法利用小牛血清白蛋白(BSA)和葡萄糖体外制备BSA-AGEs；将SH-SY5Y细胞与不同浓度BSA-AGEs保温后,MTT法测定SH-SY5Y细胞的增殖率，流式细胞仪测定细胞凋亡和细胞周期的变化，RT-PCR检测细胞中AD相关mRNA的表达水平。结果 BSA-AGEs对SH-SY5Y细胞增殖有明显抑制作用,明显促进神经元的凋亡，细胞周期被阻滞于G1/G0期，呈药物浓度依赖性。RT-PCR结果表明，经过BSA-AGEs刺激后，IL-6、高迁移率族蛋白B1(HMGB1)、淀粉先质蛋白(APLP1) mRNA表达水平均明显升高。结论 BSA-AGEs能有效抑制SH-SY5Y细胞的增殖，促进炎症细胞因子产生，诱导细胞凋亡，提示AGEs在AD的发生与发展过程中具有促进作用。
목적 연구만기당기화종산물(AGEs)대인신경모세포류SH-SY5Y세포주증식、조망이급AD상관mRNA적영향。 방법 이용소우혈청백단백(BSA)화포도당체외제비BSA-AGEs；장SH-SY5Y세포여불동농도BSA-AGEs보온후,MTT법측정SH-SY5Y세포적증식솔，류식세포의측정세포조망화세포주기적변화，RT-PCR검측세포중AD상관mRNA적표체수평。 결과 BSA-AGEs대SH-SY5Y세포증식유명현억제작용,명현촉진신경원적조망，세포주기피조체우G1/G0기，정약물농도의뢰성。RT-PCR결과표명，경과BSA-AGEs자격후，IL-6、고천이솔족단백B1(HMGB1)、정분선질단백(APLP1) mRNA표체수평균명현승고。 결론 BSA-AGEs능유효억제SH-SY5Y세포적증식，촉진염증세포인자산생，유도세포조망，제시AGEs재AD적발생여발전과정중구유촉진작용。
Objective To study the effect of advanced glycation end products (AGEs) on the proliferation, cell cycle and apoptosis of SH-SY5Y cells, and the mRNA expression of AD related protiens. Methods Bovine serum albumin-AGEs (BSA-AGEs) were prepared by incubation of BSA with glucose in vitro. SH-SY5Y cells were incubated with different concentrations of BSA-AGEs. Cell proliferation was measured by MTT assay; cell cycle and apoptosis were determined by flow cytometry; mRNA levels of APLP1, IL-6 and HMGB-1 were determined by RT-PCR. Results After incubation with BSA-AGEs for 48 h, SH-SYSY cell proliferation was significantly inhibited and cell apoptosis was induced; the cells were found to be arrested at G1/G0; these effects were dose-dependent. RT-PCR results showed that mRNA levels of IL-6, APLP1 and HMGB-1 were significantly up-regulated. Conclusion BSA-AGEs inhibit the cell proliferation and induce the apoptosis of SH-SY5Y cells, and stimulate the expression of some pro-inflammatory cytokines, suggesting a possible important role of AGEs in pathogenesis and development of AD.