中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
34期
2430-2434
,共5页
于亚峰%翟丰%戴春富%胡金家
于亞峰%翟豐%戴春富%鬍金傢
우아봉%적봉%대춘부%호금가
老年性聋%海马%突触
老年性聾%海馬%突觸
노년성롱%해마%돌촉
Presbycusis%Hippocampus%Synapses
目的 通过对老年性聋模型鼠C57BL/6J和对照组CBA/CaJ小鼠听力检测，认知行为检测以及海马CA3区突触超微结构的观察，探讨C57BL/6J小鼠年龄相关性听力损失与认知功能下降的关系。方法 将C57BL/6J小鼠根据听力随年龄的变化，按年龄分为3组：6～8周、24～26周、42～44周，每组10只。CBA/CaJ小鼠按同样的年龄也分为3组，每组9只。以TDT-Ⅲ型ABR测试仪检测听力，Morris水迷宫实验检测认知行为，透射电镜观察海马突触超微结构。结果 C57 BL/6J小鼠24～ 26周组与4～6周组比较高频开始出现听力下降，42～44周组是3个组中听力最差的。而3组的CBA/CaJ小鼠均保持了较好的听力。42～44周组的C57 BL/6J小鼠与同龄CBA/CaJ小鼠的认知行为比较，在定位航行实验中前者的潜伏期和上台前路程明显长，尤其是第3天和第4天。在空间探索实验中，42～44周组的C57 BL/6J小鼠与同龄的CBA/CaJ小鼠比较穿越平台次数明显少(0.5±0.6比1.9±1.6；P＜0. 05)。海马CA3区在42～44周C57 BL/6J小鼠比24～ 26周小鼠突触间隙明显宽[(19.4 ±0. 5)nm比(11.9±0.7) nm]，突触后致密物明显薄[(15.2±0.5) nm比(27.8±2.0) nm]，差异均有统计学意义（均P＜0. 05）。而在CBA/CaJ小鼠变化不明显。结论 C57BL/6J小鼠随年龄增加听力进一步下降，42～44周的C57B L/6J小鼠的听力接近全聋。同时42～ 44周C57BL/6J小鼠出现明显的认知功能下降及海马突触退变，而听力较好的同龄CBA/CaJ小鼠却没有出现相应的变化。我们推测C57BL/6J小鼠年龄相关的听力损失同其认知功能下降呈正相关。
目的 通過對老年性聾模型鼠C57BL/6J和對照組CBA/CaJ小鼠聽力檢測，認知行為檢測以及海馬CA3區突觸超微結構的觀察，探討C57BL/6J小鼠年齡相關性聽力損失與認知功能下降的關繫。方法 將C57BL/6J小鼠根據聽力隨年齡的變化，按年齡分為3組：6～8週、24～26週、42～44週，每組10隻。CBA/CaJ小鼠按同樣的年齡也分為3組，每組9隻。以TDT-Ⅲ型ABR測試儀檢測聽力，Morris水迷宮實驗檢測認知行為，透射電鏡觀察海馬突觸超微結構。結果 C57 BL/6J小鼠24～ 26週組與4～6週組比較高頻開始齣現聽力下降，42～44週組是3箇組中聽力最差的。而3組的CBA/CaJ小鼠均保持瞭較好的聽力。42～44週組的C57 BL/6J小鼠與同齡CBA/CaJ小鼠的認知行為比較，在定位航行實驗中前者的潛伏期和上檯前路程明顯長，尤其是第3天和第4天。在空間探索實驗中，42～44週組的C57 BL/6J小鼠與同齡的CBA/CaJ小鼠比較穿越平檯次數明顯少(0.5±0.6比1.9±1.6；P＜0. 05)。海馬CA3區在42～44週C57 BL/6J小鼠比24～ 26週小鼠突觸間隙明顯寬[(19.4 ±0. 5)nm比(11.9±0.7) nm]，突觸後緻密物明顯薄[(15.2±0.5) nm比(27.8±2.0) nm]，差異均有統計學意義（均P＜0. 05）。而在CBA/CaJ小鼠變化不明顯。結論 C57BL/6J小鼠隨年齡增加聽力進一步下降，42～44週的C57B L/6J小鼠的聽力接近全聾。同時42～ 44週C57BL/6J小鼠齣現明顯的認知功能下降及海馬突觸退變，而聽力較好的同齡CBA/CaJ小鼠卻沒有齣現相應的變化。我們推測C57BL/6J小鼠年齡相關的聽力損失同其認知功能下降呈正相關。
목적 통과대노년성롱모형서C57BL/6J화대조조CBA/CaJ소서은력검측，인지행위검측이급해마CA3구돌촉초미결구적관찰，탐토C57BL/6J소서년령상관성은력손실여인지공능하강적관계。방법 장C57BL/6J소서근거은력수년령적변화，안년령분위3조：6～8주、24～26주、42～44주，매조10지。CBA/CaJ소서안동양적년령야분위3조，매조9지。이TDT-Ⅲ형ABR측시의검측은력，Morris수미궁실험검측인지행위，투사전경관찰해마돌촉초미결구。결과 C57 BL/6J소서24～ 26주조여4～6주조비교고빈개시출현은력하강，42～44주조시3개조중은력최차적。이3조적CBA/CaJ소서균보지료교호적은력。42～44주조적C57 BL/6J소서여동령CBA/CaJ소서적인지행위비교，재정위항행실험중전자적잠복기화상태전로정명현장，우기시제3천화제4천。재공간탐색실험중，42～44주조적C57 BL/6J소서여동령적CBA/CaJ소서비교천월평태차수명현소(0.5±0.6비1.9±1.6；P＜0. 05)。해마CA3구재42～44주C57 BL/6J소서비24～ 26주소서돌촉간극명현관[(19.4 ±0. 5)nm비(11.9±0.7) nm]，돌촉후치밀물명현박[(15.2±0.5) nm비(27.8±2.0) nm]，차이균유통계학의의（균P＜0. 05）。이재CBA/CaJ소서변화불명현。결론 C57BL/6J소서수년령증가은력진일보하강，42～44주적C57B L/6J소서적은력접근전롱。동시42～ 44주C57BL/6J소서출현명현적인지공능하강급해마돌촉퇴변，이은력교호적동령CBA/CaJ소서각몰유출현상응적변화。아문추측C57BL/6J소서년령상관적은력손실동기인지공능하강정정상관。
Objective To explore the correlation of age-related hearing loss and cognition impairment in C57BL/6J mice by observing hearing, cognitive function and synapses. Methods C57BL/6J and CBA/CaJ mice were divided into 3 groups. The hearing and cognitive functions of each animal was tested. And the ultrastructure of synapses was simultaneously observed for C57BL/6J mice. Results The 24-26-week-old C57BL/6J mice developed moderate hearing loss while the 42- 44-week-old C57BL/6J counterparts suffered profound hearing loss. Whereas excellent hearing was maintained in 3 groups of CBA/CaJ mice within 44 weeks. During cognitive test, the performance of 42 -44-week-old C57BL/6J mice was significantly worse than CBA/CaJ mice. During probe test, the number of platform crossing of 42 - 44-week-old C57BL/6J mice was smaller than that of CBA/CaJ mice(0.5 ± 0.6 vs 1.9 ± 1.6; P ＜ 0.05 ). The 42 -44-week-old C57BL/6J mice had a wider synaptic cleft and a thinner postsynaptic density than the 24 -26-week-old C57 BL/6J counterparts [synaptic cleft: ( 19.4 ± 0.5 ) nm vs ( 1 1. 9 ± 0.7 ) nm; postsynaptic density:(15.2 ±0. 5) nm vs (27.8 ±2.0) nm; both P ＜0.05]. Furthermore, the degeneration of synapses in hippocampus CA3 area of C57BL/6J mice were clearly observed at 42 -44 weeks of age, but not seen in CBA/CaJ mice. Conclusion Age-related hearing loss might impact on the cognition impairment in C57 BL/6J mice.
