中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
6期
1098-1101
,共4页
梗阻性黄疽%甲硫氨酸维B1%丹参酮ⅡA磺酸钠%肿瘤坏死因子%超氧化物歧化酶
梗阻性黃疽%甲硫氨痠維B1%丹參酮ⅡA磺痠鈉%腫瘤壞死因子%超氧化物歧化酶
경조성황저%갑류안산유B1%단삼동ⅡA광산납%종류배사인자%초양화물기화매
Obstructive jaundice%Methionine and vitamin B1%Sodium tanshinone Ⅱ A sulfonate%Tumor necrosis factor-α%Superoxide dismutase
目的 观察梗阻性黄疸(OJ)大鼠血浆肿瘤坏死因子-α(TNF-α)、肾功能、肾组织丙二醛(MDA)与超氧化物歧化酶(SOD)水平变化及甲硫氨酸维B1(MVB1)与丹参酮ⅡA磺酸钠(STS)对OJ大鼠血浆TNF-α水平、肾功能、肾组织MDA与SOD变化的影响.方法 以大鼠胆总管结扎(BDL)为OJ模型,设立假手术组(Sham,n=5),将成功模型随机分为实验对照(EC)组、MVB1组、STS组及MVB1与STS联合(M-S)组,每组n=20,术后1d给BDL大鼠腹腔内注射相应剂量药物至13 d,采用放射免疫法测定BDL大鼠2、6、10、14 d血浆TNF-α、尿素氮(BUN)、Cr水平及14d肾组织MDA、SOD水平,观察死亡率与肾组织病理学改变.结果 Sham组血浆TNF-α、BUN、肌酐(Cr)与肾组织MDA、SOD分别为(0.91 ±0.18) μg/L、( 1.54±0.16) mmol/L、( 44.23±4.73) μmol/L、(4.54±0.46) nmol/ml、(182.16±12.10)U/ml,BDL后大鼠上述检测指标的变化差异有统计学意义(P＜0.01)；与EC组比较,STS组对血浆TNF-α、BUN、Cr及肾组织MDA、SOD影响差异有统计学意义,MV B1组血浆TNF-α、BUN、Cr水平变化差异有统计学意义；MVB1与STS组比较,对血浆BUN、Cr及肾MDA、SOD水平影响差异有统计学意义；M-S组与MVB1、STS组比较,血浆TNF-α、BUN、Cr与肾组织MDA水平明显下降,SOD水平显著升高(P＜0.01).光镜下:BDL后大鼠肾小球充血,上皮细胞、内皮细胞、系膜细胞增生,肾小管胆色素沉积,上皮片状坏死,间质炎细胞浸润,MVB1组、STS组、M-S组与EC组比较差异有统计学意义.结论 OJ大鼠血浆TNF-α与肾组织MDA水平进行性升高及SOD水平显著下降是导致肾功能损害的重要因素之一,MVB1和STS对OJ大鼠血浆TNF-α水平升高有抑制作用,STS可减少OJ时氧自由基损害作用,STS与MVB1联合应用表现出更有效的抗内毒素血症与改善肾组织缺血缺氧的作用.
目的 觀察梗阻性黃疸(OJ)大鼠血漿腫瘤壞死因子-α(TNF-α)、腎功能、腎組織丙二醛(MDA)與超氧化物歧化酶(SOD)水平變化及甲硫氨痠維B1(MVB1)與丹參酮ⅡA磺痠鈉(STS)對OJ大鼠血漿TNF-α水平、腎功能、腎組織MDA與SOD變化的影響.方法 以大鼠膽總管結扎(BDL)為OJ模型,設立假手術組(Sham,n=5),將成功模型隨機分為實驗對照(EC)組、MVB1組、STS組及MVB1與STS聯閤(M-S)組,每組n=20,術後1d給BDL大鼠腹腔內註射相應劑量藥物至13 d,採用放射免疫法測定BDL大鼠2、6、10、14 d血漿TNF-α、尿素氮(BUN)、Cr水平及14d腎組織MDA、SOD水平,觀察死亡率與腎組織病理學改變.結果 Sham組血漿TNF-α、BUN、肌酐(Cr)與腎組織MDA、SOD分彆為(0.91 ±0.18) μg/L、( 1.54±0.16) mmol/L、( 44.23±4.73) μmol/L、(4.54±0.46) nmol/ml、(182.16±12.10)U/ml,BDL後大鼠上述檢測指標的變化差異有統計學意義(P＜0.01)；與EC組比較,STS組對血漿TNF-α、BUN、Cr及腎組織MDA、SOD影響差異有統計學意義,MV B1組血漿TNF-α、BUN、Cr水平變化差異有統計學意義；MVB1與STS組比較,對血漿BUN、Cr及腎MDA、SOD水平影響差異有統計學意義；M-S組與MVB1、STS組比較,血漿TNF-α、BUN、Cr與腎組織MDA水平明顯下降,SOD水平顯著升高(P＜0.01).光鏡下:BDL後大鼠腎小毬充血,上皮細胞、內皮細胞、繫膜細胞增生,腎小管膽色素沉積,上皮片狀壞死,間質炎細胞浸潤,MVB1組、STS組、M-S組與EC組比較差異有統計學意義.結論 OJ大鼠血漿TNF-α與腎組織MDA水平進行性升高及SOD水平顯著下降是導緻腎功能損害的重要因素之一,MVB1和STS對OJ大鼠血漿TNF-α水平升高有抑製作用,STS可減少OJ時氧自由基損害作用,STS與MVB1聯閤應用錶現齣更有效的抗內毒素血癥與改善腎組織缺血缺氧的作用.
목적 관찰경조성황달(OJ)대서혈장종류배사인자-α(TNF-α)、신공능、신조직병이철(MDA)여초양화물기화매(SOD)수평변화급갑류안산유B1(MVB1)여단삼동ⅡA광산납(STS)대OJ대서혈장TNF-α수평、신공능、신조직MDA여SOD변화적영향.방법 이대서담총관결찰(BDL)위OJ모형,설립가수술조(Sham,n=5),장성공모형수궤분위실험대조(EC)조、MVB1조、STS조급MVB1여STS연합(M-S)조,매조n=20,술후1d급BDL대서복강내주사상응제량약물지13 d,채용방사면역법측정BDL대서2、6、10、14 d혈장TNF-α、뇨소담(BUN)、Cr수평급14d신조직MDA、SOD수평,관찰사망솔여신조직병이학개변.결과 Sham조혈장TNF-α、BUN、기항(Cr)여신조직MDA、SOD분별위(0.91 ±0.18) μg/L、( 1.54±0.16) mmol/L、( 44.23±4.73) μmol/L、(4.54±0.46) nmol/ml、(182.16±12.10)U/ml,BDL후대서상술검측지표적변화차이유통계학의의(P＜0.01)；여EC조비교,STS조대혈장TNF-α、BUN、Cr급신조직MDA、SOD영향차이유통계학의의,MV B1조혈장TNF-α、BUN、Cr수평변화차이유통계학의의；MVB1여STS조비교,대혈장BUN、Cr급신MDA、SOD수평영향차이유통계학의의；M-S조여MVB1、STS조비교,혈장TNF-α、BUN、Cr여신조직MDA수평명현하강,SOD수평현저승고(P＜0.01).광경하:BDL후대서신소구충혈,상피세포、내피세포、계막세포증생,신소관담색소침적,상피편상배사,간질염세포침윤,MVB1조、STS조、M-S조여EC조비교차이유통계학의의.결론 OJ대서혈장TNF-α여신조직MDA수평진행성승고급SOD수평현저하강시도치신공능손해적중요인소지일,MVB1화STS대OJ대서혈장TNF-α수평승고유억제작용,STS가감소OJ시양자유기손해작용,STS여MVB1연합응용표현출경유효적항내독소혈증여개선신조직결혈결양적작용.
Objective To observe changes of kidney function,tumor necrosis factor-α (TNF-α)in plasma and renal superoxide dismutase (SOD) and malondialdehyde (MDA) of rats with obstructive jaundice (OJ) and effects of sodium tanshinone Ⅱ A sulfonate (STS) and methionine and vitamin B1 ( MVB1 ).Methods Sham-operation group was set up ( n =5 ),and OJ models were established by ligation of common bile duct in rats.OJ rats were randomly divided into experimental control group ( EC group,n =20),MVB1 group (n=20),STS group (n =20) and MVB1 and STS group (M-S group,n=20).From 1st to 13 day,the rate in MVB1 and STS groups were respectively injected with the same volume of isotonic sodium hloride,MVB1 and STS,respectively.The rats in M-S group received MVB1 and STS together.The expression levels of TNF-α,blood urea nitrogen (BUN) and creatinine (Cr) in plasma on the day 2,6,10 and 14 after operation,MDA and SOD in the kidney tissue on the day 14 were determined by using radioimmunoassay.The histopathological changes of kidney and mortality rate of rats were observed.Results In sham group,levels of TNF-α,BUN and Cr in plasma and MDA and SOD in the kidney tissue were (0.91 ±0.18) μg/L,(1.54±0.16) mmol/L,(44.23 ±4.73) μ mol/L,(4.54±0.46) nmol/ml,and ( 182.16 ± 12.10) U/ml respectively,In the OJ groups,there was significant chants inthe above indicators (P ＜ 0.01 ).As compared with EC group,levels of TNF-α,BUN,Cr and MDA were declined and those of SOD were increased in STS group ( P ＜ 0.05).There was significant changes in TNF-α,BUN and Cr in MVB1 grorp (P ＜0.05).There was statistically significnat difference in the levels of BUN,Cr,MDA and SOD between MVB1 and STS groups.Levels of TNF-α,BUN,Cr and MDA in M-S group were markedly lower than those in sham group,MVB1 and STS group (P ＜ 0.01 ),and levels of SOD were significantly higher.Under the light microscopy,histological examination showed hyperplasia of glomerular capillary endothelial cells,epithelial cells and mesangial cells,patchy necrosis,bile pigment deposition in renal tubules,and inflammatory cell infiltration.There were significant difference in histological changes between EC group with MVB1,STS,and M-S groups.Conclusion That levels of TNF-α in plasma and MDA in the kidney tissue were rapidly elevated and SOD decreased was one of the most important factors of damaging renal function in OJ rats.MVB1 and STS could restrain the increase of plasma TNF-α in OJ rats.STS has effects of protecting renal tissue in OJ rats from damaging by oxygen-derived free radicals.Combined use of MVB1 and STS exerts the syngistic effects,and the antiendotoxin effects were significantly enhanced.
