CAJ | 학술논문

目的观察猕猴牵张成骨术整复腭裂过程中,新骨组织形成与改建活动的特点,探讨新骨生成的规律.方法建立猕猴腭裂动物模型,以牵张成骨术整复其腭部软硬组织缺损,以每天2次、每次0.4 mm的速率牵引,直至骨运送盘移动关闭裂隙后原位固定.分别于固定期第1、2、4、6、8、12及24周取材,每时相点3只实验动物.取材前6 d,实验组动物(21只)均肌肉注射四环素(30 mg/kg)标记.各组标本包埋切片行组织学及荧光标记观察.结果与实验对照组及空白对照组(动物各2只,同法标记)对比.结果实验组骨运送盘移动至裂隙对侧关闭缺损,牵开间隙内以膜内成骨方式原位成骨,成功整复腭部软硬组织缺损.牵张成骨术后固定期从1至24周逐渐延长,呈现出成骨-改建-成熟的动态变化过程:牵张区新骨形成的量及其钙化程度不断增加,与此同时,新骨组织亦呈现不断改建成熟的趋势,软组织随着新骨的形成不断地延伸,对照组裂隙无法自行修复.结论应用牵张成骨术整复腭裂缺损,在良好的固定下,通过膜内成骨的方式,新骨不断的成熟和改建,直至成功修复软硬组织缺损.
목적 관찰미후견장성골술정복악렬과정중,신골조직형성여개건활동적특점,탐토신골생성적규률.방법 건립미후악렬동물모형,이견장성골술정복기악부연경조직결손,이매천2차、매차0.4 mm적속솔견인,직지골운송반이동관폐렬극후원위고정.분별우고정기제1、2、4、6、8、12급24주취재,매시상점3지실험동물.취재전6 d,실험조동물(21지)균기육주사사배소(30 mg/kg)표기.각조표본포매절편행조직학급형광표기관찰.결과 여실험대조조급공백대조조(동물각2지,동법표기)대비.결과 실험조골운송반이동지렬극대측관폐결손,견개간극내이막내성골방식원위성골,성공정복악부연경조직결손.견장성골술후고정기종1지24주축점연장,정현출성골-개건-성숙적동태변화과정:견장구신골형성적량급기개화정도불단증가,여차동시,신골조직역정현불단개건성숙적추세,연조직수착신골적형성불단지연신,대조조렬극무법자행수복.결론 응용견장성골술정복악렬결손,재량호적고정하,통과막내성골적방식,신골불단적성숙화개건,직지성공수복연경조직결손.
Objective To investigate the mechanism of new bone formation in the distraction osteogenesis (DO) for correction of cleft palate(CP) in rhesus. Methods CP was created by operation in 23 rhesus. The CP was corrected with DO in 21 animals as experimental group. The distraction rate was 0.8mm per day, two times a day. The bone fragments were fixed after cleft closure, every 3 animals were sacrified to get specimen after 1, 2, 4, 6, 8, 12, 24 weeks of fixation. 6 days before sacrifice,tetracycline was administrated for labeling (30 mg/kg). Results The hard and soft tissue def of fixation.At the same time, the bone volume and calcification between the distraction gap increased. The cleft in the control group could not b set was successfully closed with DO by intramembrane osteogenesis. The new formed bone was remodeling and became maturation during the period e corrected spontaneously.Conclusions The DO can successfully correct both the soft and hard tissue defect in CP by intramembrane osteogenesis. The fixation is important for remodeling and maturation of the new formed bone.