CAJ | 학술논문

目的:探讨脐血千细胞移植对大鼠脊髓损伤后轴突再生的影响.方法:收集脐带血,分离提取,制备成合适浓度的CD34阳性细胞.Allen重物坠击法制作SD大鼠急性脊髓损伤动物模型30只,随机分成2组.A组为损伤对照组,B组为细胞移植组,1周后再次手术,细胞移植组将体外培养的脐血干细胞用微量注射器分别注入于脊髓损伤区域头侧和尾侧各10x105个细胞,损伤对照组予以同样体积的PBS,于移植后1周、2周、6周时分别取材,进行组织学检查及免疫组织化学检杏以评估损伤区域脊髓形态学变化,并于移植后1周、2周、4周、6周采用BBB评分及斜板实验,以观察大鼠后肢运动神经功能恢复情况.结果:与对照组比较,细胞移植组大鼠脊髓损伤区域空腔面积显著减小,生长相关蛋白(GAP-43)和神经丝蛋白(NF200)表达显著增加,并且后肢运动功能均有较明显的恢复.结论:脐血干细胞移植可以促进脊髓损伤大鼠轴突再生和后肢运动功能恢复.
목적:탐토제혈천세포이식대대서척수손상후축돌재생적영향.방법:수집제대혈,분리제취,제비성합괄농도적CD34양성세포.Allen중물추격법제작SD대서급성척수손상동물모형30지,수궤분성2조.A조위손상대조조,B조위세포이식조,1주후재차수술,세포이식조장체외배양적제혈간세포용미량주사기분별주입우척수손상구역두측화미측각10x105개세포,손상대조조여이동양체적적PBS,우이식후1주、2주、6주시분별취재,진행조직학검사급면역조직화학검행이평고손상구역척수형태학변화,병우이식후1주、2주、4주、6주채용BBB평분급사판실험,이관찰대서후지운동신경공능회복정황.결과:여대조조비교,세포이식조대서척수손상구역공강면적현저감소,생장상관단백(GAP-43)화신경사단백(NF200)표체현저증가,병차후지운동공능균유교명현적회복.결론:제혈간세포이식가이촉진척수손상대서축돌재생화후지운동공능회복.
Objective: To explore the effect of umbilical cord blood stem cell transplantation on axon regeneration in spinal cord injury (SCl)in rats. Methods: The umbilical cord blood was collected and prepared into suitable concentration of CD34 positive stem cells. Thirty SD rats were divided into two groups randomly. One group served as control, another one was the treatment group. The models of spinal cord contusion injury were made by Allen's weight dropping method. One week later,the treatment group was transplanted with 10x105 umbilical cord blood stem cells with Hamilton micro-syringe at the sites of rostral and caudal to the lesioned zone respectively, while control group received just the same volume of PBS injection. Five rats in each group were sacrificed at 1 w, 2 w and 6 w after this operation. Histological and immunohistochemieal examinations including GAP-43 and NF200 were used to evaluate axon regeneration. Meanwhile, BBB motion scoring and inclined plane test were performed to assess the motion function changes of hindlimbs. Results: Compared to the control group, the area of cavity in the lesioned spinal cord region decreased significantly and the expressions of GAP-43 and NF200 increased markedly in cell transplantation group. Also the motion function had better restoration in the treatment group. Conclusion: Transplantation of umbilical cord blood stem cell may achieve both morphological and behavioral improvement for the injured spinal cord.