CAJ | 학술논문

建立了一种固相萃取-超高效液相色谱法(SPE-UPLC)快速检测主流烟气中丙烯酰胺的方法. 使用剑桥滤片和吸收瓶捕集主流烟气后, 蒸馏水做萃取溶剂, 采用C_(18)固相萃取小柱对样品液进行纯化, 用UPLC检测, 外标法定量. UPLC方法采用ACQUITY UPLC~(TM) BEH C18 1.7 μm 2.1×50 mm色谱柱, 柱温30 ℃, 流动相为V(乙腈)∶V(水)＝6∶94, 流速为0.15 mL/min, 紫外检测器(TUV)检测波长为202 nm, 分析时间为6 min. 烤烟型香烟主流烟气中丙烯酰胺的含量为4.75 μg/cig. 方法的线性范围为0.1～10 mg/mL, 线性相关系数为0.9999;平均回收率为98.7%;检出限为10 ng/mL (S/N=3);相对标准偏差为2.3%. 该方法适合主流烟气中丙烯酰胺的快速检测.
건립료일충고상췌취-초고효액상색보법(SPE-UPLC)쾌속검측주류연기중병희선알적방법. 사용검교려편화흡수병포집주류연기후, 증류수주췌취용제, 채용C_(18)고상췌취소주대양품액진행순화, 용UPLC검측, 외표법정량. UPLC방법채용ACQUITY UPLC~(TM) BEH C18 1.7 μm 2.1×50 mm색보주, 주온30 ℃, 류동상위V(을정)∶V(수)＝6∶94, 류속위0.15 mL/min, 자외검측기(TUV)검측파장위202 nm, 분석시간위6 min. 고연형향연주류연기중병희선알적함량위4.75 μg/cig. 방법적선성범위위0.1～10 mg/mL, 선성상관계수위0.9999;평균회수솔위98.7%;검출한위10 ng/mL (S/N=3);상대표준편차위2.3%. 해방법괄합주류연기중병희선알적쾌속검측.
A new method for rapid determination of acrylamide in mainstream cigarette smoke by SPE-UPLC was developed . After collected in mainstream cigarette smoke by Cambridge filter pad and absorption bottle, the total particulate matte was extracted by distilled water, and purified by solid phase extraction, then detected by UPLC and the quantitative analysis was performed by the external standard method. The optimal experimental conditions were as follows: the column was ACQU1TY UPLC~(TM) BEH C_(18) 1.7 pm 2.1×50 mm column, mobile phase was mixture of acetonitrile and water (6=94, V: V), column temperature was 30 t, TUV detection wavelength was at 202 ran, the analytical time was 6 min. The quantity of acrylamide in flue-cured cigarette mainstream smoke was determined to be 4.75 jug/mL by the method. The calibration curve showed good linearity in the rang of 0.1～10 mg/mL for acrylamind with a correlation coefficient of 0.9999; the average recovery of the method was 98.7%; the detection limit was 10 ng/mL (S/N = 3), RSDwas2.3%. The method was simple, rapid, accurate and well suitable for rapid detection of acrylamide in mainstream cigarette smoke.