CAJ | 학술논문

采用响应曲面法对粗壮脉纹孢菌降解啤酒糟粗纤维的培养基进行优化.通过Plackett-Burman设计法及响应面分析法,评价不同比例CaCl_2、KH_2PO_4、豆渣、(NH_4)_2SO_4、MgSO_4、尿素等6个因素对粗壮脉纹孢菌降解啤酒糟粗纤维的影响.研究表明,豆渣、(NH_4)_2SO_4、KH_2PO_4、CaCl_2、MgSO_4是粗壮脉纹孢菌降解啤酒糟粗纤维过程中的主要影响因素.得到优化培养基:豆渣为26.08%、(NH_4)_2SO_4为3.71%、KH_2PO_4为1.53%,CaCl_2为0.24%、MgSO_4为0.08%,在此条件下重复发酵3次,结果与理论预测值相近.
채용향응곡면법대조장맥문포균강해비주조조섬유적배양기진행우화.통과Plackett-Burman설계법급향응면분석법,평개불동비례CaCl_2、KH_2PO_4、두사、(NH_4)_2SO_4、MgSO_4、뇨소등6개인소대조장맥문포균강해비주조조섬유적영향.연구표명,두사、(NH_4)_2SO_4、KH_2PO_4、CaCl_2、MgSO_4시조장맥문포균강해비주조조섬유과정중적주요영향인소.득도우화배양기:두사위26.08%、(NH_4)_2SO_4위3.71%、KH_2PO_4위1.53%,CaCl_2위0.24%、MgSO_4위0.08%,재차조건하중복발효3차,결과여이론예측치상근.
In an attempt to optimize the culture medium for fibre degradation of brewers' grains by Neurospora crass, Plackett-Burman design and central composite design combined with response surface methodology were employed in this work. First, the effects of soybean residues, (NH_4)_2SO_4, urea, KH_2PO_4, CaCl_2 and MgSO_4 on fibre degradation by Neurospora crassa were evaluated by a Plackett-Burman design leading to 8 experiments and the most important affecting factors were screened. Subsequently, the interactive effects of the screened factors on fibre degradation were determined using response surface methodology based on a five-variable, five-level central composite design involving 32 experiments. Results indicated that CaCl_2, KH_2PO_4 soybean residues, (NH_4)_2SO_4 and MgSO_4 were among the most important affecting factors of fibre degradation. The optimal culture medium consisted of 26.08% soybean residues, 3.71% (NH_4)2SO_4, 1.53% KH_2PO_4, 0.24% CaCl_2 and 0.08% MgSO_4. Three replicate fermentations using the optimized culture medium all gave a value of fibre degradation rate which was in good agreement with the predicted value.