中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2010年
6期
651-652
,共2页
聚合酶链反应%培养%解脲支原体%婴儿%新生
聚閤酶鏈反應%培養%解脲支原體%嬰兒%新生
취합매련반응%배양%해뇨지원체%영인%신생
Polymerase chain reaction%Cultur%Ureaplasma urealyticum%Infants%newborn
目的 探讨荧光定量聚合酶链反应(FQ-PCR)法和液体培养法检测新生儿解脲支原体(Uu)的临床意义.方法 采用FQ-PCR法和液体培养法检测新生儿痰中Uu,比较两者阳性检出率,及治疗后Uu含量的变化.结果 FQ-PCR法、液体培养法Uu阳性检出率分别为32.87%、22.69%,FQ-PCR法明显高于液体培养法(χ2=5.58,P＜0.05).治疗后Uu阳性检出率为8.33%,且拷贝数明显下降.Uu对大环内酯类克拉霉素敏感率最高,为93.88%;对喹诺酮类环丙沙星耐药率最高,为89.80%.结论 FQ-PCR联合液体培养法检测Uu对诊治新生儿Uu感染有临床指导意义.
目的 探討熒光定量聚閤酶鏈反應(FQ-PCR)法和液體培養法檢測新生兒解脲支原體(Uu)的臨床意義.方法 採用FQ-PCR法和液體培養法檢測新生兒痰中Uu,比較兩者暘性檢齣率,及治療後Uu含量的變化.結果 FQ-PCR法、液體培養法Uu暘性檢齣率分彆為32.87%、22.69%,FQ-PCR法明顯高于液體培養法(χ2=5.58,P＜0.05).治療後Uu暘性檢齣率為8.33%,且拷貝數明顯下降.Uu對大環內酯類剋拉黴素敏感率最高,為93.88%;對喹諾酮類環丙沙星耐藥率最高,為89.80%.結論 FQ-PCR聯閤液體培養法檢測Uu對診治新生兒Uu感染有臨床指導意義.
목적 탐토형광정량취합매련반응(FQ-PCR)법화액체배양법검측신생인해뇨지원체(Uu)적림상의의.방법 채용FQ-PCR법화액체배양법검측신생인담중Uu,비교량자양성검출솔,급치료후Uu함량적변화.결과 FQ-PCR법、액체배양법Uu양성검출솔분별위32.87%、22.69%,FQ-PCR법명현고우액체배양법(χ2=5.58,P＜0.05).치료후Uu양성검출솔위8.33%,차고패수명현하강.Uu대대배내지류극랍매소민감솔최고,위93.88%;대규낙동류배병사성내약솔최고,위89.80%.결론 FQ-PCR연합액체배양법검측Uu대진치신생인Uu감염유림상지도의의.
Objective To discuss the clinical significance of the fluorescence quantitative polymerase chain reaction (FQ-PCR) with liquid culture in detecting neonatal Ureaplasma urealyticum (Uu).Methods Neonatal Uu in sputa specimens was separately detected by FQ-PCR and liquid culture.The results were compared between these two methods.The changes of Uu levels were also observed after treatment.Results The positive rate of neonatal Uu was 32.87% (by FQ-PCR) or 22.69% (by liquid culture) (P＜0.05).The positive rate of Uu was 8.33% after treatment.Uu were most sensitive to clarithromycin (response rate: 93.88%) and most resistant to ciprofloxacin (resistance rate:89.80%).Conclusion FQ-PCR combined with liquid culture can provide valuable information in the detection and treatment of neonatal Uu.
