CAJ | 학술논문

利用稀释培养法从缫丝废水中分离到5株可降解家蚕蛹油的细菌,采用紫外分光光度法测定了其对家蚕蛹油的降解能力,H73、H23、H1、H5和H43菌株在72 h内对家蚕蛹油的降解率分别达到76.36%、62.04%、60.58%、53.85%和51.20%.通过形态特征、生理生化特性测定和16S rDNA序列系统发生分析对各菌株进行鉴定,其中H23、H1和H43为芽孢杆菌属(Bacillus sp.),H5为微杆菌属(Microbacterium sp.),H73为肠杆菌属(Enterobacter sp.).
이용희석배양법종소사폐수중분리도5주가강해가잠용유적세균,채용자외분광광도법측정료기대가잠용유적강해능력,H73、H23、H1、H5화H43균주재72 h내대가잠용유적강해솔분별체도76.36%、62.04%、60.58%、53.85%화51.20%.통과형태특정、생리생화특성측정화16S rDNA서렬계통발생분석대각균주진행감정,기중H23、H1화H43위아포간균속(Bacillus sp.),H5위미간균속(Microbacterium sp.),H73위장간균속(Enterobacter sp.).
Five bacterial strains capable of degrading silkworm pupal oil were isolated from the waste water of a silk reeling factory by dilution plate method. Determination of their degrading capability with UV spectrophotometry indicated that strains H73, H23, H1, H5 and H43 had a degradation rate of 76.36%, 62.04%, 60.58%, 53.85% and 51.20% in 72 hours, respectively. The strains were identified based on morphological characters, physiological and biochemical properties, and phylogenetic analysis on their 16S rDNA sequences. It was found that strains H23, H1 and H43 belonged to Bacillus sp. , strain H5 belonged to Microbacterium sp. , and strain H73 belonged to Enterobacter sp..