中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
3期
308-311
,共4页
丁晨光%田普训%薛武军%管智慧%葛冠群%靳占奎%李钊伦%贾利宁%丁小明%潘晓明%燕航
丁晨光%田普訓%薛武軍%管智慧%葛冠群%靳佔奎%李釗倫%賈利寧%丁小明%潘曉明%燕航
정신광%전보훈%설무군%관지혜%갈관군%근점규%리쇠륜%가리저%정소명%반효명%연항
茯苓素%急性排斥反应%肾脏移植%环孢素A
茯苓素%急性排斥反應%腎髒移植%環孢素A
복령소%급성배척반응%신장이식%배포소A
Poria cocos%acute rejection%renal transplantation%ciclosporin A
目的 探讨茯苓素(Poria cocos)预防大鼠肾脏移植急性排斥反应的作用和机制.方法 以Wistar大鼠为供者,SD大鼠为受者,建立原位(腹腔)肾脏移植模型,移植前按照分组分别以生理盐水、茯苓素25mg·kg~(-1)·d~(-1)、茯苓素50 mg·kg~(-1)·d~(-1)和环孢素A(CsA)5 mg·kg~(-1)·d~(-1)灌胃.术后观察大鼠移植肾功能和存活时间,测定各组术后1周外周血中白细胞介素2(IL-2)和γ干扰素(IFN-γ)含量以及CD_4~+、CD_8~+细胞百分比和CD_4~+/CD_8~+的比值,并观察移植肾的病理变化.结果 茯苓素50 mg·kg~(-1)·d~(-1)组和CsA组移植肾和受者存活时间较茯苓素25 mg·kg~(-1)·d-1~(-1)组和生理盐水组显著延长(P＜0.05),而且病理损害程度较茯苓素25 mg·kg~(-1)·d~(-1)组和生理盐水组明显减轻,但茯苓素50 mg·kg~(-1)·d~(-1)组较CsA组移植肾存活时间显著减少(P＜0.05).各组移植肾功能和尿量变化与存活时间和移植肾病理变化相一致,茯苓素50 mg·kg~(-1)·d~(-1)组和CsA组肾功能异常发生时间明显晚于茯苓素25 mg·kg~(-1)·d~(-1)组和生理盐水组.茯苓素50 mg·kg~(-1)·d~(-1)组外周血IL-2和IFN-r的含量较茯苓素25 mg·kg~(-1)·d~(-1)组和生理盐水组显著降低(P＜0.01),但显著高于CsA组(P＜0.01);茯苓素50 mg·kg~(-1)·d~(-1)组外周血CD4+细胞百分比显著低于茯苓素25 mg·kg~(-1)·d~(-1)组(P＜0.05)和生理盐水组(P＜0.01),CD8+细胞百分比显著低于生理盐水组(P＜0.05).结论 茯芩素对肾脏移植急性排斥反应有较好的抑制作用且与剂量呈正相关,但效果不如CsA.
目的 探討茯苓素(Poria cocos)預防大鼠腎髒移植急性排斥反應的作用和機製.方法 以Wistar大鼠為供者,SD大鼠為受者,建立原位(腹腔)腎髒移植模型,移植前按照分組分彆以生理鹽水、茯苓素25mg·kg~(-1)·d~(-1)、茯苓素50 mg·kg~(-1)·d~(-1)和環孢素A(CsA)5 mg·kg~(-1)·d~(-1)灌胃.術後觀察大鼠移植腎功能和存活時間,測定各組術後1週外週血中白細胞介素2(IL-2)和γ榦擾素(IFN-γ)含量以及CD_4~+、CD_8~+細胞百分比和CD_4~+/CD_8~+的比值,併觀察移植腎的病理變化.結果 茯苓素50 mg·kg~(-1)·d~(-1)組和CsA組移植腎和受者存活時間較茯苓素25 mg·kg~(-1)·d-1~(-1)組和生理鹽水組顯著延長(P＜0.05),而且病理損害程度較茯苓素25 mg·kg~(-1)·d~(-1)組和生理鹽水組明顯減輕,但茯苓素50 mg·kg~(-1)·d~(-1)組較CsA組移植腎存活時間顯著減少(P＜0.05).各組移植腎功能和尿量變化與存活時間和移植腎病理變化相一緻,茯苓素50 mg·kg~(-1)·d~(-1)組和CsA組腎功能異常髮生時間明顯晚于茯苓素25 mg·kg~(-1)·d~(-1)組和生理鹽水組.茯苓素50 mg·kg~(-1)·d~(-1)組外週血IL-2和IFN-r的含量較茯苓素25 mg·kg~(-1)·d~(-1)組和生理鹽水組顯著降低(P＜0.01),但顯著高于CsA組(P＜0.01);茯苓素50 mg·kg~(-1)·d~(-1)組外週血CD4+細胞百分比顯著低于茯苓素25 mg·kg~(-1)·d~(-1)組(P＜0.05)和生理鹽水組(P＜0.01),CD8+細胞百分比顯著低于生理鹽水組(P＜0.05).結論 茯芩素對腎髒移植急性排斥反應有較好的抑製作用且與劑量呈正相關,但效果不如CsA.
목적 탐토복령소(Poria cocos)예방대서신장이식급성배척반응적작용화궤제.방법 이Wistar대서위공자,SD대서위수자,건립원위(복강)신장이식모형,이식전안조분조분별이생리염수、복령소25mg·kg~(-1)·d~(-1)、복령소50 mg·kg~(-1)·d~(-1)화배포소A(CsA)5 mg·kg~(-1)·d~(-1)관위.술후관찰대서이식신공능화존활시간,측정각조술후1주외주혈중백세포개소2(IL-2)화γ간우소(IFN-γ)함량이급CD_4~+、CD_8~+세포백분비화CD_4~+/CD_8~+적비치,병관찰이식신적병리변화.결과 복령소50 mg·kg~(-1)·d~(-1)조화CsA조이식신화수자존활시간교복령소25 mg·kg~(-1)·d-1~(-1)조화생리염수조현저연장(P＜0.05),이차병리손해정도교복령소25 mg·kg~(-1)·d~(-1)조화생리염수조명현감경,단복령소50 mg·kg~(-1)·d~(-1)조교CsA조이식신존활시간현저감소(P＜0.05).각조이식신공능화뇨량변화여존활시간화이식신병리변화상일치,복령소50 mg·kg~(-1)·d~(-1)조화CsA조신공능이상발생시간명현만우복령소25 mg·kg~(-1)·d~(-1)조화생리염수조.복령소50 mg·kg~(-1)·d~(-1)조외주혈IL-2화IFN-r적함량교복령소25 mg·kg~(-1)·d~(-1)조화생리염수조현저강저(P＜0.01),단현저고우CsA조(P＜0.01);복령소50 mg·kg~(-1)·d~(-1)조외주혈CD4+세포백분비현저저우복령소25 mg·kg~(-1)·d~(-1)조(P＜0.05)화생리염수조(P＜0.01),CD8+세포백분비현저저우생리염수조(P＜0.05).결론 복금소대신장이식급성배척반응유교호적억제작용차여제량정정상관,단효과불여CsA.
Objective To study the effect of Poria cocos (Poe) in preventing acute rejection of rats after renal transplantation and its mechanism.Methods Rat orthotopic renal transplantation model was performed with Wistar rat as donor and SD rat as donee.All donees were divided into 4 groups,10 in each group,before transplantation.They were treated respectively with normal saline 5 mL~(-1)· kg~(-1)·d~(-1) (A),Pcs 25 mg·kg~(-1)·d~(-1)(B),Pcs 50 mg·kg~(-1)·d~(-1) (C) and ciclosporin A (CsA) 5 mg·kg~(-1)·d~(-1) (D) by intragastric administration.The renal allograft survival time (ST) was recorded,and the serum levels of creatinine(SCr),interleukin-2(IL-2),γ-interferon (γ-IFN),CD_4~+,CD_8~+ lymphocytes percentage,CD_4~+/CD_8~+ ratio,as well as the pathologic changes were observed one week after transplantation.Results ST of the renal graft in Groups C and D was significantly longer with pathologic change evidently less than those in Groups A and B(P＜0.01),and the ST in Group C was shorter that in Group D (P＜0.05).Changes of renal function and urine volume were identified to the pathological change of graft,the initiating time of renal dysfunction was later in Groups C and D than that in Groups A and B.Serum levels of IL-2,IFN-y and CD4+ percentage in Group C were significantly lower than those in Groups A and B,but higher than those in Group D respectively(P＜0.05 or P＜0.01),while CD8+ percentage in Group C was significantly lower than that in Group A (P＜0.05),but insignificantly different to that in Groups B and D(P＞0.05).Conclusion Pcs shows good dosage-dependent effect in suppressing acute rejection of renal transplantation,but the effect is inferior to that of CsA.
