CAJ | 학술논문

目的：评价薄层液基细胞学及HPV16/18型原位分子杂交检测在子宫颈病变中的应用价值。方法：应用薄层液基细胞学技术及HE染色对112例患者行子宫颈脱落细胞学检查,按照TBS（theBethesda report system）细胞学分类标准分类：无上皮内病变或恶性病变（negative for introepithelia or ma-lignancy,NILM）,意义不明确的非典型鳞状上皮细胞（atypical squamous cell of undetermined significance,ASC-US）、低度鳞状上皮内病变（Low-grade squamous intraepithelial lesion,LSIL）、高度鳞状上皮内病变（High-grade squamous intraepithelial lesion,HSIL）,鳞状细胞癌（squamous cell carcinoma,SCC）。同时应用原位分子杂交方法对样本进行HPV16/18型检测。结果：①在112例细胞涂片中,NILM：16例（14.3%）;炎症病变：26例（23.2%）;ASC-US：44例（39.3%）;LSIL：25例（22.3%）;HSIL：1例（0.89%）;②112例细胞涂片中HPV16/18型阳性率随着子宫颈病变加重而增高,NILM及炎症、ASC-US、LSIL及HSIL各组间相比,NILM及炎症病变组与LSIL及HSIL组之间表达差异有统计学意义（P〈0.05）;③对5例细胞学HPV16/18型胞浆阳性病例的子宫颈活检标本进行HPV原位杂交对照检查,结果显示细胞核均呈阳性。结论：子宫颈液基细胞学联合HPV16/18型原位分子杂交检测有助于发现HPV感染引起的子宫颈病变;HPV16/18型细胞涂片中胞浆阳性染色,可作为细胞学检查HPV阳性的判定指标。
목적：평개박층액기세포학급HPV16/18형원위분자잡교검측재자궁경병변중적응용개치。방법：응용박층액기세포학기술급HE염색대112례환자행자궁경탈락세포학검사,안조TBS（theBethesda report system）세포학분류표준분류：무상피내병변혹악성병변（negative for introepithelia or ma-lignancy,NILM）,의의불명학적비전형린상상피세포（atypical squamous cell of undetermined significance,ASC-US）、저도린상상피내병변（Low-grade squamous intraepithelial lesion,LSIL）、고도린상상피내병변（High-grade squamous intraepithelial lesion,HSIL）,린상세포암（squamous cell carcinoma,SCC）。동시응용원위분자잡교방법대양본진행HPV16/18형검측。결과：①재112례세포도편중,NILM：16례（14.3%）;염증병변：26례（23.2%）;ASC-US：44례（39.3%）;LSIL：25례（22.3%）;HSIL：1례（0.89%）;②112례세포도편중HPV16/18형양성솔수착자궁경병변가중이증고,NILM급염증、ASC-US、LSIL급HSIL각조간상비,NILM급염증병변조여LSIL급HSIL조지간표체차이유통계학의의（P〈0.05）;③대5례세포학HPV16/18형포장양성병례적자궁경활검표본진행HPV원위잡교대조검사,결과현시세포핵균정양성。결론：자궁경액기세포학연합HPV16/18형원위분자잡교검측유조우발현HPV감염인기적자궁경병변;HPV16/18형세포도편중포장양성염색,가작위세포학검사HPV양성적판정지표。
Objective： To evaluate the application value of liquid-based cytology and HPV 16/18 detection by in situ hybridization in cervical lesions.Methods： 112 cases were examined with liquid-based thin-layer cytology and stained with HE.According to the classification standard of TBS,the cervical lesions were divided into negative for intraepithelial or malignancy（NILM）;atypical squamous cell of undetermined significance（ASC-US）;Low-grade squamous intraepithelial lesion（LSIL）;High-grade squamous intraepithelial lesion（HSIL） and squamous cell carcinoma（SCC）.And HPV 16/18 of 112 cases were detected by in situ hybridization.Results： ① In the 112 cases,16 cases（14.3%） were NILM;26 cases（23.2%） were inflammatory;44 cases（39.3%） were ASC-US;25 cases（22.3%） were LSIL;1 case（0.89%） was HSIL.② With the lesion aggravating,the positive rate of HPV 16/18 increased gradually.And there was a significant difference（P0.05） between NILM,inflammation and LSIL,HSIL.③ In cervical cytology,HPV 16/18 positive expression was located in cytoplasm in 5 cervical biopsy specimens.And it was also located in nucleus detected by in situ hybridization in the same specimens.Conclusions： Liquid-based cytology combining with HPV 16/18 detection by in situ hybridization is helpful for testing cervical lesion;cytoplasm HPV 16/18 positive staining may be used as a positive assessment marker in cervical cytology.