CAJ | 학술논문

本文采用稀盐酸水解玉米麸皮得到低聚糖阿魏酸酯(FOs),经D301大孔树脂的除杂和聚酰胺柱层析的分离获得FOs中的F-Ara组分,在分析结构后,对该组分进行了体外清除DPPH和超氧阴离子自由基的能力测定和还原力测定.结果表明,与标准物阿魏酸FA)、BHA和维生素C相比,F-Ara均表现出良好的体外清除自由基活性,其活性随浓度的增加而增大；在试验浓度为0.6 mmol/L时,与标准物阿魏酸和维生素C相比,F-Ara显示出最高的还原值,说明F-Ara具有较强的还原力.以上结果揭示F-Ara可成为一种能应用到食品、药品和化妆品的天然高效的抗氧化剂.
본문채용희염산수해옥미부피득도저취당아위산지(FOs),경D301대공수지적제잡화취선알주층석적분리획득FOs중적F-Ara조분,재분석결구후,대해조분진행료체외청제DPPH화초양음리자자유기적능력측정화환원력측정.결과표명,여표준물아위산FA)、BHA화유생소C상비,F-Ara균표현출량호적체외청제자유기활성,기활성수농도적증가이증대；재시험농도위0.6 mmol/L시,여표준물아위산화유생소C상비,F-Ara현시출최고적환원치,설명F-Ara구유교강적환원력.이상결과게시F-Ara가성위일충능응용도식품、약품화화장품적천연고효적항양화제.
In this study feruloylated oligosaccharides(FOs)was released from maize bran by hydrochlonc acid hydrolysis,and feruloyl arabinose(F-Ara)was obtained by D301 macroporous resin chromatography followed by polyamide resin purification from FOs.After structural identification,the antioxidant activity of F-Ara was evaluated in vitro by DPPH and superoxide radical scavenging activity assay and reducing power assay.F-Ara exhibited a good radical-scavenging activity in vitro when compared to standard antioxidants such as ferulic acid (FA),BHA and L-ascorbic acid(VC).The radical-scavenging activity depended on the concentration and increases with increasing dose of sample.The highest reducing value of F-Ara compared with FA and VC at a dosage of 0.6 mmol/L indicated that F-Ara had a considerable reducing power.The present study suggested that F-Ara can be a natural and efficient antioxidant used in food,medicine and cosmetic.