中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2011年
9期
1184-1187
,共4页
寿华锋%倪镌%陈雅卿%孙海燕
壽華鋒%倪鐫%陳雅卿%孫海燕
수화봉%예전%진아경%손해연
逆转录聚合酶链反应%宫颈肿瘤/代谢%角蛋白19/代谢
逆轉錄聚閤酶鏈反應%宮頸腫瘤/代謝%角蛋白19/代謝
역전록취합매련반응%궁경종류/대사%각단백19/대사
Reverse transcriptase polymerase chain reaction%Uterine cervical neoplasms/ME%Keratin-19/ME
目的 探讨宫颈癌患者外周血中CK19mRNA的表达及其临床意义.方法 应用荧光定量逆转录聚合酶链式反应(FQ-RT-PCR)技术检测138例经病理证实的宫颈癌及36例妇科良性肿瘤(对照组)外周血液中CK19mRNA的表达,并分析其与临床病理因素的关系.结果 138例宫颈癌组外周血CK19阳性率(69.6％)高于对照组(13.9％,x2=36.34,P=0.000).宫颈癌组中早期宫颈癌(Ⅰ-ⅡA期)外周血CK19 mRNA阳性率(57.9％)低于中晚期宫颈癌(Ⅱb-Ⅳ期,80.6％,x2=8.14,P=0.004).早期宫颈癌患者CK19 mRNA的表达与年龄(＜40岁、≥40岁)、肿瘤大小(＜4cm、≥≥4 cm)、病理类型(鳞癌、非鳞癌)、浸润深度、淋巴转移(+/-)均无关(P＞0.05)；与肿瘤分期(ⅠA、ⅠB、ⅡA)、组织分化(G1 -2、G3)、脉管瘤栓(+/-)有关(x2=9.59,7.27,9.94,P＜0.01).早期宫颈癌患者Logistica多因素分析示CK19 mRNA的表达与脉管瘤栓相关(x2 =5.29,P=0.021).结论 荧光定量RT-PCR技术可检测出各期宫颈癌患者外周血中CK19的表达,其敏感性和特异性高,可作为检测宫颈癌微转移的生物学指标,对判断宫颈癌的预后具有一定指导意义.
目的 探討宮頸癌患者外週血中CK19mRNA的錶達及其臨床意義.方法 應用熒光定量逆轉錄聚閤酶鏈式反應(FQ-RT-PCR)技術檢測138例經病理證實的宮頸癌及36例婦科良性腫瘤(對照組)外週血液中CK19mRNA的錶達,併分析其與臨床病理因素的關繫.結果 138例宮頸癌組外週血CK19暘性率(69.6％)高于對照組(13.9％,x2=36.34,P=0.000).宮頸癌組中早期宮頸癌(Ⅰ-ⅡA期)外週血CK19 mRNA暘性率(57.9％)低于中晚期宮頸癌(Ⅱb-Ⅳ期,80.6％,x2=8.14,P=0.004).早期宮頸癌患者CK19 mRNA的錶達與年齡(＜40歲、≥40歲)、腫瘤大小(＜4cm、≥≥4 cm)、病理類型(鱗癌、非鱗癌)、浸潤深度、淋巴轉移(+/-)均無關(P＞0.05)；與腫瘤分期(ⅠA、ⅠB、ⅡA)、組織分化(G1 -2、G3)、脈管瘤栓(+/-)有關(x2=9.59,7.27,9.94,P＜0.01).早期宮頸癌患者Logistica多因素分析示CK19 mRNA的錶達與脈管瘤栓相關(x2 =5.29,P=0.021).結論 熒光定量RT-PCR技術可檢測齣各期宮頸癌患者外週血中CK19的錶達,其敏感性和特異性高,可作為檢測宮頸癌微轉移的生物學指標,對判斷宮頸癌的預後具有一定指導意義.
목적 탐토궁경암환자외주혈중CK19mRNA적표체급기림상의의.방법 응용형광정량역전록취합매련식반응(FQ-RT-PCR)기술검측138례경병리증실적궁경암급36례부과량성종류(대조조)외주혈액중CK19mRNA적표체,병분석기여림상병리인소적관계.결과 138례궁경암조외주혈CK19양성솔(69.6％)고우대조조(13.9％,x2=36.34,P=0.000).궁경암조중조기궁경암(Ⅰ-ⅡA기)외주혈CK19 mRNA양성솔(57.9％)저우중만기궁경암(Ⅱb-Ⅳ기,80.6％,x2=8.14,P=0.004).조기궁경암환자CK19 mRNA적표체여년령(＜40세、≥40세)、종류대소(＜4cm、≥≥4 cm)、병리류형(린암、비린암)、침윤심도、림파전이(+/-)균무관(P＞0.05)；여종류분기(ⅠA、ⅠB、ⅡA)、조직분화(G1 -2、G3)、맥관류전(+/-)유관(x2=9.59,7.27,9.94,P＜0.01).조기궁경암환자Logistica다인소분석시CK19 mRNA적표체여맥관류전상관(x2 =5.29,P=0.021).결론 형광정량RT-PCR기술가검측출각기궁경암환자외주혈중CK19적표체,기민감성화특이성고,가작위검측궁경암미전이적생물학지표,대판단궁경암적예후구유일정지도의의.
Objective To detect the expression of Cytokeratin 19 (CK19) mRNA in the peripheral blood of cervical carcinoma patients and evaluate its clinical significance.Methods The expression of CK19 mRNA was evaluated by fluorescent quantitative reverse transcription polymerase chain reaction ( FQRT-PCR ) in the peripheral blood of 138 patients with cervical carcinoma and 36 patients with benign gynecological tumors.In 138 patients,possible correlations between clinical pathological factors were analyzed.Results The positive expression rates of CK19 mRNA was 69.6％ in 138 cervical carcinomas in comparison with 13.9％ in benign gynecological tumors,and 57.9％ in patients with FIGO Stage Ⅰ A to Ⅱ A cervical carcinoma in comparison with 80.6％ in patients with FIGO Stage Ⅱb to Ⅳ cervical carcinoma.The expression of CK19 mRNA in patients with FIGO Stage Ⅰ A to Ⅱ A cervical carcinoma were significantly correlated with stage,differentiation and lymph vascular space involvement,but was not associated with prognostic factors including age,bully tumor size,pathological types,deep stromal invasion and lymph node metastasis.In multivariate survival analysis,lymph vascular space involvement was an independent risk factor of CK19mRNA expression in patients with cervical carcinoma.Conclusions Fluorescent quantitative RTPCR can be used to detect the expression of CK19 in the peripheral blood of cervical carcinoma patients,and it is a sensitive and specific technique.CK19 mRNA in peripheral blood may be a potential biomarker for detecting micrometastasis in cervical carcinoma.The results suggest a possible use of this approach for evaluating prognosis.
