CAJ | 학술논문

目的比较分离自褐家鼠的汉滩病毒(HTNV)CGRn5310株和汉城病毒(SEOV)HR54株的毒力差异,探索汉坦病毒(HV)"溢出"到非宿主动物后的毒力变化.方法选取从贵州省褐家鼠中分离的CGRn5310株HTNV和从河南省褐家鼠分离的HR54株SEOV,用不同稀释度的病毒悬液经脑内接种于昆明乳鼠,观察小鼠发病症状,测定其半数致死量(LD_(50)),并用免疫荧光法检测脑与肺组织内HV特异抗原.结果接种2株病毒后的小鼠生长缓慢,均出现不同程度的神经症状.CGRn5310株的LD_(50)是10~-6.42,HR54株的LD_(50)是10~-4.51.在死亡小鼠的脑与肺组织中均能检测到HV特异抗原,而对照组脑与肺组织内未检测到病毒抗原.结论从褐家鼠中分离到的CGRn5310株HTNV的毒力大于从褐家鼠中分离到HR54株SEOV,这可能意味着HV"溢出"到非宿主动物后毒力变化较小.
목적 비교분리자갈가서적한탄병독(HTNV)CGRn5310주화한성병독(SEOV)HR54주적독력차이,탐색한탄병독(HV)"일출"도비숙주동물후적독력변화.방법 선취종귀주성갈가서중분리적CGRn5310주HTNV화종하남성갈가서분리적HR54주SEOV,용불동희석도적병독현액경뇌내접충우곤명유서,관찰소서발병증상,측정기반수치사량(LD_(50)),병용면역형광법검측뇌여폐조직내HV특이항원.결과 접충2주병독후적소서생장완만,균출현불동정도적신경증상.CGRn5310주적LD_(50)시10~-6.42,HR54주적LD_(50)시10~-4.51.재사망소서적뇌여폐조직중균능검측도HV특이항원,이대조조뇌여폐조직내미검측도병독항원.결론 종갈가서중분리도적CGRn5310주HTNV적독력대우종갈가서중분리도HR54주SEOV,저가능의미착HV"일출"도비숙주동물후독력변화교소.
Objective Comparing the difference of virulence between the strain CGRn5310 (HTNV) and the strain HR54 (SEOV) isolated both from Rnttus norvegicus. Methods Suckling mice were used to compare the difference of virulence between the two strains. Hantavirus antigens were detected in brain and lung tissues collected from the infected mice. Results Compared with the control group, all infected mice grew slowly. Furthermore, the mice inoculated intracerebrally with either CGRn5310 or HR45 appeared ruffled fur, and reduced activity, followed by neurological symptoms, such as paralyses and convulsions. The half lethal dose (LD_(50)) of CGRn5310 strain was 10~-6.42, whereas the LD_(50) of HR54 strain was 10~-4.51. Hantavirus antigens were identified in brain and lung tissues from the mice infected with the strain CGRn5310 and the strain HR54. Conclusion LD_(50) of the strain CGRn5310 was significantly higher than that of the strain HR54. Our results suggested that the virulence of the spillover hantavirus might only slightly be influenced by the non-reservoir rodents.