中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2011年
9期
742-746
,共5页
刘稳%高志强%神平%冯国栋%查洋%张彦东%刘佩毛
劉穩%高誌彊%神平%馮國棟%查洋%張彥東%劉珮毛
류은%고지강%신평%풍국동%사양%장언동%류패모
疱疹病毒1型,人%Bell麻痹%运动神经元%细胞凋亡%bcl-2相关X蛋白质%疾病模型,动物
皰疹病毒1型,人%Bell痳痺%運動神經元%細胞凋亡%bcl-2相關X蛋白質%疾病模型,動物
포진병독1형,인%Bell마비%운동신경원%세포조망%bcl-2상관X단백질%질병모형,동물
Herpesvirns 1,human%Bell palsy%Motor neurons%Apoptosis%bcl-2-associated X protein%Disease models,animal
目的 探讨1型单纯疱疹病毒（herpes simplex virus type 1，HSV-1）性面神经炎动物模型中面神经运动神经元（facial motor neurons,FMN）的凋亡情况以及凋亡相关基因bcl-2和bax的表达。方法 Balb/c小鼠84只，分别进行HSV-1接种和面神经切断处理。在操作后第1、3、7、10、15、20及30天分批处死动物，对面神经核进行HE染色、尼氏染色；采用原位末端转移酶标记（terminaldeoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling，TUNEL）技术检测FMN的凋亡；免疫组化染色检测凋亡相关基因bcl-2和bax在FMN中的表达。结果 面神经切断后，同侧FMN出现凋亡，在第10、15天达高峰，Bcl-2表达降低，Bax表达升高，Bcl-2/Bax比值下降；接种病毒后，同侧FMN没有明显的细胞凋亡，Bcl-2表达增高，在第15天达高峰，Bcl-2/Bax比值上升。结论 小鼠面神经接种HSV-1后FMN没有明显凋亡，可能与神经损伤程度轻以及HSV-1抑制宿主细胞凋亡有关，Bcl-2、Bax可能参与调控FMN的凋亡。
目的 探討1型單純皰疹病毒（herpes simplex virus type 1，HSV-1）性麵神經炎動物模型中麵神經運動神經元（facial motor neurons,FMN）的凋亡情況以及凋亡相關基因bcl-2和bax的錶達。方法 Balb/c小鼠84隻，分彆進行HSV-1接種和麵神經切斷處理。在操作後第1、3、7、10、15、20及30天分批處死動物，對麵神經覈進行HE染色、尼氏染色；採用原位末耑轉移酶標記（terminaldeoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling，TUNEL）技術檢測FMN的凋亡；免疫組化染色檢測凋亡相關基因bcl-2和bax在FMN中的錶達。結果 麵神經切斷後，同側FMN齣現凋亡，在第10、15天達高峰，Bcl-2錶達降低，Bax錶達升高，Bcl-2/Bax比值下降；接種病毒後，同側FMN沒有明顯的細胞凋亡，Bcl-2錶達增高，在第15天達高峰，Bcl-2/Bax比值上升。結論 小鼠麵神經接種HSV-1後FMN沒有明顯凋亡，可能與神經損傷程度輕以及HSV-1抑製宿主細胞凋亡有關，Bcl-2、Bax可能參與調控FMN的凋亡。
목적 탐토1형단순포진병독（herpes simplex virus type 1，HSV-1）성면신경염동물모형중면신경운동신경원（facial motor neurons,FMN）적조망정황이급조망상관기인bcl-2화bax적표체。방법 Balb/c소서84지，분별진행HSV-1접충화면신경절단처리。재조작후제1、3、7、10、15、20급30천분비처사동물，대면신경핵진행HE염색、니씨염색；채용원위말단전이매표기（terminaldeoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling，TUNEL）기술검측FMN적조망；면역조화염색검측조망상관기인bcl-2화bax재FMN중적표체。결과 면신경절단후，동측FMN출현조망，재제10、15천체고봉，Bcl-2표체강저，Bax표체승고，Bcl-2/Bax비치하강；접충병독후，동측FMN몰유명현적세포조망，Bcl-2표체증고，재제15천체고봉，Bcl-2/Bax비치상승。결론 소서면신경접충HSV-1후FMN몰유명현조망，가능여신경손상정도경이급HSV-1억제숙주세포조망유관，Bcl-2、Bax가능삼여조공FMN적조망。
Objective To study the apoptosis of facial motor neurons and the expression of apoptosis-related genes, Bcl-2 and Bax, in the animal model of viral facial paralysis. Methods Total of 84Balb/c mice were divided into viral inoculation group and nerve transaction group. The animals were executed 1, 3, 7, 10, 15, 20 and 30 days after being operated respectively. The histopathological features of facial neurons in brain stem were observed by HE and Nissl stain. The changes of facial neuronal apoptosis were observed by TUNEL The changes of expression of Bcl-2 and Bax genes in facial neurons were observed by immunohistochemistry staining. Results After nerve transection, increased apoptotic cells were found in homolateral facial motor neucleus and the peak appeared at 10 and 15 days. The level of Bcl-2 expression inneurons declined while the expression of Bax increased gradually. Correspondingly, the ratio of Bcl-2/Bax declined. In the viral inoculation group, no visible change of apoptosis and Bax expression, but the level of Bcl-2 and the ratio of Bcl-2/Bax increased gradually. Conclusions Comparing to axotomy, facial motor neucleus in HSV-1 infective animal model are free of apoptosis. Both the mild form of lesion and the ability to block apoptosis of HSV-1 are likely to be involved into the phenomenon. Bcl-2 and Bax might interfere with the apoptotic response.
