中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2009年
2期
145-147
,共3页
血红素氧合酶%脑外伤%保护作用
血紅素氧閤酶%腦外傷%保護作用
혈홍소양합매%뇌외상%보호작용
Heine oxygenase-1%Brain trauma%Protection
目的 探讨血红素氧合酶-1(HO-1)在颅脑外伤中的作用. 方法 48只SD大鼠按照随机数字表法分为血晶素处理组、生理盐水组和假手术组.用液压冲击伤复制颅脑外伤的大模型,伤后予以45mg/100mg体质量血晶素、生理盐水腹腔注射干预HO-1的表达.假手术组只切开头皮,颅骨钻孔.其他不作任何处理.斜坡试验观测大的行为学改变.尾静脉注射伊文思兰测定血脑屏障的通透性.SP免疫组织化学测定HO-1的表达. 结果 血晶素处理组HO-1的表达[(125.52±14.39)个]较生理盐水组[(100.63±12.32)个]和假手术组[(79.37±12.89)个]明显增加,差异有统计学意义(P<0.05).血晶素处理组大鼠斜坡试验时间[(7.38±1.69)s]较生理盐水组[(10.01±1.61)s]缩短,差异有统计学意义(P<0.05).血晶素处理组血脑屏障的通透性较生理盐水组明显降低.差异有统计学意义(P<0.05). 结论 HO-1在颅脑外伤中的表达对中枢神经系统具有保护作用.
目的 探討血紅素氧閤酶-1(HO-1)在顱腦外傷中的作用. 方法 48隻SD大鼠按照隨機數字錶法分為血晶素處理組、生理鹽水組和假手術組.用液壓遲擊傷複製顱腦外傷的大模型,傷後予以45mg/100mg體質量血晶素、生理鹽水腹腔註射榦預HO-1的錶達.假手術組隻切開頭皮,顱骨鑽孔.其他不作任何處理.斜坡試驗觀測大的行為學改變.尾靜脈註射伊文思蘭測定血腦屏障的通透性.SP免疫組織化學測定HO-1的錶達. 結果 血晶素處理組HO-1的錶達[(125.52±14.39)箇]較生理鹽水組[(100.63±12.32)箇]和假手術組[(79.37±12.89)箇]明顯增加,差異有統計學意義(P<0.05).血晶素處理組大鼠斜坡試驗時間[(7.38±1.69)s]較生理鹽水組[(10.01±1.61)s]縮短,差異有統計學意義(P<0.05).血晶素處理組血腦屏障的通透性較生理鹽水組明顯降低.差異有統計學意義(P<0.05). 結論 HO-1在顱腦外傷中的錶達對中樞神經繫統具有保護作用.
목적 탐토혈홍소양합매-1(HO-1)재로뇌외상중적작용. 방법 48지SD대서안조수궤수자표법분위혈정소처리조、생리염수조화가수술조.용액압충격상복제로뇌외상적대모형,상후여이45mg/100mg체질량혈정소、생리염수복강주사간예HO-1적표체.가수술조지절개두피,로골찬공.기타불작임하처리.사파시험관측대적행위학개변.미정맥주사이문사란측정혈뇌병장적통투성.SP면역조직화학측정HO-1적표체. 결과 혈정소처리조HO-1적표체[(125.52±14.39)개]교생리염수조[(100.63±12.32)개]화가수술조[(79.37±12.89)개]명현증가,차이유통계학의의(P<0.05).혈정소처리조대서사파시험시간[(7.38±1.69)s]교생리염수조[(10.01±1.61)s]축단,차이유통계학의의(P<0.05).혈정소처리조혈뇌병장적통투성교생리염수조명현강저.차이유통계학의의(P<0.05). 결론 HO-1재로뇌외상중적표체대중추신경계통구유보호작용.
Objective To explore the role of heme oxygenase-1 (HO-1) in the central nervous system after brain trauma. Methods Forty-eight adult SD rats were randomized into brain trauma+hemin group, brain trauma+normal saline group and sham-operated group, and in the former two groups, the rats were subjected to fluid percussion injury of the brain followed by intraperitoneal injections of 45 mg/100 mg hemin or saline accordingly. The rats in the sham-operated group received incision of the scalp and drilling on the skull only. The behavioral changes of the rats after the treatments were evaluated by testing the latency of head turning on a ramp. The permeability of the blood brain barrier (BBB) was assessed by measuring the brain tissue content of Evans blue injected into the tail vein, and the expression of HO-1 in the brain tissue was detected using immunohistochemistry with SP method. Results HO-1 expression in the brain tissue of heroin-treated rats (125.52±14.39) was significantly increased as compared with that of normal saline-treated rats (100.63±12.32) and sham-operated rats (79.37±12.89) (P<0.05). The latency of head turning on the ramp was significantly shorter in hemin-treated group (7.38±1.69 s) than in normal saline group (10.01±1.61 s,P<0.05). The permeability of the BBB was markedly decreased in heroin group. Conclusion HO-1 expression in the brain tissue may provide protection of central nervous system after brain trauma.
