CAJ | 학술논문

目的观察糖尿病性勃起功能障碍(DMED)大鼠的睾酮水平变化对其阴茎海绵体平滑肌组织和超微结构的影响.方法将3个月龄雄性Wistar大鼠20只随机等分为两组:正常对照组和DMED组,行放射免疫法测定其血清睾酮浓度和masson染色观察阴茎海绵体平滑肌(CCSM)密度以及通过透射电镜观察海绵体平滑肌细胞的超微结构.结果 DMED组大鼠的血清睾酮水平(17.445 ±2.540) nmol/L较正常组(338.457±63.802) nmol/L明显下降(P＜0.01)；同时DMED组大鼠CCSM密度(5.640±0.597)％较正常对照组(19.890±1.957)％亦明显减少(P＜0.01),透射电镜下还观察到DMED组大鼠阴茎CSMC的核浆比增大,高尔基复合体明显肥大以及线粒体水肿.结论睾酮水平下降可能导致DMED大鼠阴茎海绵体平滑肌发生病理变化.
목적 관찰당뇨병성발기공능장애(DMED)대서적고동수평변화대기음경해면체평활기조직화초미결구적영향.방법 장3개월령웅성Wistar대서20지수궤등분위량조:정상대조조화DMED조,행방사면역법측정기혈청고동농도화masson염색관찰음경해면체평활기(CCSM)밀도이급통과투사전경관찰해면체평활기세포적초미결구.결과 DMED조대서적혈청고동수평(17.445 ±2.540) nmol/L교정상조(338.457±63.802) nmol/L명현하강(P＜0.01)；동시DMED조대서CCSM밀도(5.640±0.597)％교정상대조조(19.890±1.957)％역명현감소(P＜0.01),투사전경하환관찰도DMED조대서음경CSMC적핵장비증대,고이기복합체명현비대이급선립체수종.결론 고동수평하강가능도치DMED대서음경해면체평활기발생병리변화.
Objective To investigate the changes of testosterone levels and the effect of low level testosterone on the corpus cavernosum tissue and its ultrastructure in rats with diabetes mellitus-induced erectile dysfunction.Methods Three-month-old male Wistar rats ( n =20) were divided into 2 groups:the contorl group (n =10) and the diabetes mellitus-induced erectile dysfunction group (n =10).Serum levels of testosterone were measured by radioimmunoassay and the percentage of corporal smooth muscle cells was detected by Masson staining.The ultrastructures of corporal smooth muscle cells were examined under the transmission electron microscopy.Results The serum levels of testosterone in the diabetes mellitus-induced erectile dysfunction group ( 17.445 ± 2.540) nmol/L were obviously lower than in the contorl group (338.457 ± 63.802) nmol/L,P ＜ 0.01.The percentage of corporal smooth muscle cells ( 19.890 ±1.957) ％ was higher in the control group than in the diabetes mellitus-induced erectile dysfunction group (5.640 ± 0.597)％,P ＜0.01.Ultrastructurally,in the smooth muscle cells of cavernous tissue,there were more Goigi bodies and secretory vacuoles,and the karyoplasmic ratio was higher in the diabetes mellitus-induced erectile dysfunction group than in the control group.Aslo the degeneration of mitochondria,the dilatation of endoplasmic reticulum and the decreases of glycogen particles and microfilaments could be observed.Conclusion The decline of testosterone levels may lead to the pathological changes of corpus caveruosum cells in rats with diabetes mellitus-induced erectile dysfunction.