CAJ | 학술논문

应用正交设计研究了IBA、6-BA、GA3和CM等植物生长调节剂在龙眼(Dimocarpus longana Lour.)与荔枝(Litchi chinensis Sonn.)细胞悬浮培养中的作用,并就悬浮培养细胞对Km的敏感性以及用金刚沙对胚性愈伤组织在液体状态下进行创伤和用分别带有AP1和APBD基因的农杆菌对创伤的愈伤组织进行遗传转化进行了探讨.结果表明:IBA、6-BA、GA3和CM均可显著地提高悬浮培养系的产量,但GA3导致细胞的快速生长和褐变.悬浮培养系细胞在液体培养基中比在固体培养基中对Km较敏感.用金刚沙对胚性愈伤组织在液体状态下进行创伤后进行农杆菌介导转化可以得到更多的Km抗性胚状体.PCR检测结果显示,AP1及AP-D基因均已转入龙眼的胚状体中.
응용정교설계연구료IBA、6-BA、GA3화CM등식물생장조절제재용안(Dimocarpus longana Lour.)여려지(Litchi chinensis Sonn.)세포현부배양중적작용,병취현부배양세포대Km적민감성이급용금강사대배성유상조직재액체상태하진행창상화용분별대유AP1화APBD기인적농간균대창상적유상조직진행유전전화진행료탐토.결과표명:IBA、6-BA、GA3화CM균가현저지제고현부배양계적산량,단GA3도치세포적쾌속생장화갈변.현부배양계세포재액체배양기중비재고체배양기중대Km교민감.용금강사대배성유상조직재액체상태하진행창상후진행농간균개도전화가이득도경다적Km항성배상체.PCR검측결과현시,AP1급AP-D기인균이전입용안적배상체중.
In this paper, the effects of plant growth regulators including IBA, 6-BA, GA3 and CM on cell suspension culture of litchi and longan, the susceptibility of cell suspension lines to Km and the genetic transformation through Agrobacterium tumefaciences with AP1 or APBD gene and liquid-phase wounding of embryogenic tissues with carborundum were studied. The results showed that IBA, 6-BA, GA3 and CM significantly increased the yield of cell suspension lines, whereas GA3 resulted in cell fast growing and browning. In suspension culture, cell suspension lines were found more susceptible to Km than on solidified culture. More Kmr embryoids could be obtained by the way of liquid-phase wounding of embryogenic calli with carborundum. Assay by PCR indicated that multiple genes were integrated in the genomic DNA of longan.