CAJ | 학술논문

背景:脑海马结构是与学习记忆有关的脑区,一般认为与空间认知活动密切相关.纹状体边缘区是新近发现的脑内与学习记忆功能有关的一个亚区,其学习记忆功能是否和海马的学习记忆功能有区别?目的:比较纹状体边缘区与海马两个不同脑区在学习记忆功能上的区作用及地位,观察纹状体边缘区和海马在逃避性学习记忆方面的差别.设计:完全随机分组对照实验.单位:解放军第一军医大学珠江医院神经科学研究所.材料:实验于2002-03/2003-07在解放军第一军医大学珠江医院神经科学研究所完成.选择健康雄性成年SD大鼠109只,经两次Y型迷宫实验筛选后获得合格动物75只,随机分成损毁纹状体边缘区组25只、切断双侧穹窿海马伞组10只、纹状体边缘区对照组30只和双侧穹窿海马伞对照组10只.应用Y迷宫训练大鼠后24 h,损毁纹状体边缘区组以10 g/L海人藻酸0.1～0.2μL损毁大鼠双侧纹状体边缘区;纹状体边缘区对照组以双侧纹状体边缘区微量注射生理盐水;切断双侧穹窿海马伞组切断双侧穹窿海马伞;双侧穹窿海马伞对照组仅切断双侧穹窿海马伞浅层的皮质组织.手术在第2次筛选的次日进行.术后5 d观察大鼠在Y迷宫中学习记忆的行为表现(30次迷宫测试中,正确次数≥15即为学习记忆能力正常).主要观察指标:不同组别大鼠手术前后Y迷宫学习的正确次数.结果:健康雄性成年SD大鼠109只,经两次Y型迷宫实验筛选后获得合格动物75只,实验过程中有3只死亡,还有32只因未能被准确注射药物或生理盐水到纹状体边缘区而被淘汰,最后共有40只大鼠进入分析,其中损毁纹状体边缘区组11只,纹状体边缘区对照组9只,切断双侧穹窿海马伞组10只,双侧穹窿海马伞组10只.①手术后损毁纹状体边缘区组的大鼠Y迷宫学习数据的正确次数低于纹状体边缘区对照组,切断双侧穹窿海马伞组,双侧穹窿海马伞对照组[(9.27±4.29)次,(22.56±4.25)次,(21.10±4.68)次,(22.00±4.89)次,(P=0.000)].②损毁纹状体边缘区组手术后Y迷宫学习的正确次数也显著低于手术前[(9.27±4.29)次,(18.27±3.07)次,(P=0.000)].③切断双侧穹窿海马伞组的大鼠Y迷宫学习成绩正确次数与双侧穹窿海马伞对照组及纹状体边缘区对照组基本一致(P=0.660和P=0.489),手术后Y迷宫学习的正确次数和手术前也基本一致(P=0.700).结论:损毁纹状体边缘区大鼠在Y迷宫中的学习成绩明显降低,而切断双侧穹窿海马伞大鼠学习记忆成绩与未切断穹窿海马伞大鼠比较无明显变化,证明纹状体边缘区能用反映较复杂条件反射的电Y迷宫检测学习记忆行为,而海马区则不能,进一步说明两者在调控大脑学习记忆功能上的区别,纹状体边缘区对海马的学习记忆功能有调控作用. 揹景:腦海馬結構是與學習記憶有關的腦區,一般認為與空間認知活動密切相關.紋狀體邊緣區是新近髮現的腦內與學習記憶功能有關的一箇亞區,其學習記憶功能是否和海馬的學習記憶功能有區彆?目的:比較紋狀體邊緣區與海馬兩箇不同腦區在學習記憶功能上的區作用及地位,觀察紋狀體邊緣區和海馬在逃避性學習記憶方麵的差彆.設計:完全隨機分組對照實驗.單位:解放軍第一軍醫大學珠江醫院神經科學研究所.材料:實驗于2002-03/2003-07在解放軍第一軍醫大學珠江醫院神經科學研究所完成.選擇健康雄性成年SD大鼠109隻,經兩次Y型迷宮實驗篩選後穫得閤格動物75隻,隨機分成損燬紋狀體邊緣區組25隻、切斷雙側穹窿海馬傘組10隻、紋狀體邊緣區對照組30隻和雙側穹窿海馬傘對照組10隻.應用Y迷宮訓練大鼠後24 h,損燬紋狀體邊緣區組以10 g/L海人藻痠0.1～0.2μL損燬大鼠雙側紋狀體邊緣區;紋狀體邊緣區對照組以雙側紋狀體邊緣區微量註射生理鹽水;切斷雙側穹窿海馬傘組切斷雙側穹窿海馬傘;雙側穹窿海馬傘對照組僅切斷雙側穹窿海馬傘淺層的皮質組織.手術在第2次篩選的次日進行.術後5 d觀察大鼠在Y迷宮中學習記憶的行為錶現(30次迷宮測試中,正確次數≥15即為學習記憶能力正常).主要觀察指標:不同組彆大鼠手術前後Y迷宮學習的正確次數.結果:健康雄性成年SD大鼠109隻,經兩次Y型迷宮實驗篩選後穫得閤格動物75隻,實驗過程中有3隻死亡,還有32隻因未能被準確註射藥物或生理鹽水到紋狀體邊緣區而被淘汰,最後共有40隻大鼠進入分析,其中損燬紋狀體邊緣區組11隻,紋狀體邊緣區對照組9隻,切斷雙側穹窿海馬傘組10隻,雙側穹窿海馬傘組10隻.①手術後損燬紋狀體邊緣區組的大鼠Y迷宮學習數據的正確次數低于紋狀體邊緣區對照組,切斷雙側穹窿海馬傘組,雙側穹窿海馬傘對照組[(9.27±4.29)次,(22.56±4.25)次,(21.10±4.68)次,(22.00±4.89)次,(P=0.000)].②損燬紋狀體邊緣區組手術後Y迷宮學習的正確次數也顯著低于手術前[(9.27±4.29)次,(18.27±3.07)次,(P=0.000)].③切斷雙側穹窿海馬傘組的大鼠Y迷宮學習成績正確次數與雙側穹窿海馬傘對照組及紋狀體邊緣區對照組基本一緻(P=0.660和P=0.489),手術後Y迷宮學習的正確次數和手術前也基本一緻(P=0.700).結論:損燬紋狀體邊緣區大鼠在Y迷宮中的學習成績明顯降低,而切斷雙側穹窿海馬傘大鼠學習記憶成績與未切斷穹窿海馬傘大鼠比較無明顯變化,證明紋狀體邊緣區能用反映較複雜條件反射的電Y迷宮檢測學習記憶行為,而海馬區則不能,進一步說明兩者在調控大腦學習記憶功能上的區彆,紋狀體邊緣區對海馬的學習記憶功能有調控作用.
배경:뇌해마결구시여학습기억유관적뇌구,일반인위여공간인지활동밀절상관.문상체변연구시신근발현적뇌내여학습기억공능유관적일개아구,기학습기억공능시부화해마적학습기억공능유구별?목적:비교문상체변연구여해마량개불동뇌구재학습기억공능상적구작용급지위,관찰문상체변연구화해마재도피성학습기억방면적차별.설계:완전수궤분조대조실험.단위:해방군제일군의대학주강의원신경과학연구소.재료:실험우2002-03/2003-07재해방군제일군의대학주강의원신경과학연구소완성.선택건강웅성성년SD대서109지,경량차Y형미궁실험사선후획득합격동물75지,수궤분성손훼문상체변연구조25지、절단쌍측궁륭해마산조10지、문상체변연구대조조30지화쌍측궁륭해마산대조조10지.응용Y미궁훈련대서후24 h,손훼문상체변연구조이10 g/L해인조산0.1～0.2μL손훼대서쌍측문상체변연구;문상체변연구대조조이쌍측문상체변연구미량주사생리염수;절단쌍측궁륭해마산조절단쌍측궁륭해마산;쌍측궁륭해마산대조조부절단쌍측궁륭해마산천층적피질조직.수술재제2차사선적차일진행.술후5 d관찰대서재Y미궁중학습기억적행위표현(30차미궁측시중,정학차수≥15즉위학습기억능력정상).주요관찰지표:불동조별대서수술전후Y미궁학습적정학차수.결과:건강웅성성년SD대서109지,경량차Y형미궁실험사선후획득합격동물75지,실험과정중유3지사망,환유32지인미능피준학주사약물혹생리염수도문상체변연구이피도태,최후공유40지대서진입분석,기중손훼문상체변연구조11지,문상체변연구대조조9지,절단쌍측궁륭해마산조10지,쌍측궁륭해마산조10지.①수술후손훼문상체변연구조적대서Y미궁학습수거적정학차수저우문상체변연구대조조,절단쌍측궁륭해마산조,쌍측궁륭해마산대조조[(9.27±4.29)차,(22.56±4.25)차,(21.10±4.68)차,(22.00±4.89)차,(P=0.000)].②손훼문상체변연구조수술후Y미궁학습적정학차수야현저저우수술전[(9.27±4.29)차,(18.27±3.07)차,(P=0.000)].③절단쌍측궁륭해마산조적대서Y미궁학습성적정학차수여쌍측궁륭해마산대조조급문상체변연구대조조기본일치(P=0.660화P=0.489),수술후Y미궁학습적정학차수화수술전야기본일치(P=0.700).결론:손훼문상체변연구대서재Y미궁중적학습성적명현강저,이절단쌍측궁륭해마산대서학습기억성적여미절단궁륭해마산대서비교무명현변화,증명문상체변연구능용반영교복잡조건반사적전Y미궁검측학습기억행위,이해마구칙불능,진일보설명량자재조공대뇌학습기억공능상적구별,문상체변연구대해마적학습기억공능유조공작용.
BACKGROUND: Hippocampal structure in brain is the division related to learning and memory, generally it is closely relevant to spatial cognitive activity. Marginal division of striatum is a latest discovered subdivision related to learning and memory function of brain, whether is its learning and memory function different from that in hippocampus?OBJECTIVE: To compare the difference, function and importance in learning and memory function between marginal division of striatum and hippocampus in brain and observe the difference in escape learning and memory between marginal division of striatum and hippocampus.DESIGN: Completely randomized controlled experiment.SETTING: Institute of Neuroscience in Zhujiang Hospital Affiliated to First Military Medical University of Chinese PLA.MATERIALS: The experiment was performed in Institute of Neuroscience in Zhujiang Hospital Affiliated to First Military Medical University of Chinese PLA from March 2002 to July 2003. Totally 109 normal male adult SD rats were employed and 75 rats of them were screened as the qualified animals by twice Y-maze test. Randomly, 25 rats were divided into damaged marginal division of striatum group (DMD group), 10 rats were into bilateral fimbria-fornix transection group (FFT group), 30 rats were into the control of marginal division of striatum (MD control) and 10 rats were into the control of bilateral fimbria-fornix group (FF control). After 24 hours training in Y-maze,In DMD group, 10 g/L kainic acid 0.1 to 0.2 μL was used to damage bilateral marginal division of striatum of rats. In MD control, physiological saline of minim dose was injected in bilateral marginal division of striatum of rats. In FFT group, bilateral fimbria-fornix was transectioned. In FF control, the cortical tissue of the superficial layer of bilateral fimbria-fornix was transectioned. The operation was done on the second day after the 2nd screening. The behavior of rats in learning and memory was observed in Y-maze on the 5th day after operation (during 30 times of maze test, if success frequency ≥ 15,normal capacity of learning and memory was identified.).MAIN OUTCOME MEASURES: Success frequency of rats in different groups in Y-maze learning before and after operation.RESULTS: Of 109 normal male adult SD rats, 75 rats were screened to be qualified after twice Y-maze test. During the experiment, 3 rats were died and other 32 rats fell into disuse for the drug or physiological saline was not injected accurately to the marginal division of striatum. Terminally, 40 rats entered the analysis in total, of which, 11 rats were in DMD group, 9 rats in MD quency in Y-maze learning of rats in DMD group after operation was lower than MD control, FFT group and FF control [(9.27±4.29) times, (22.56±4.25)frequency in Y-maze learning of rats in DMD group after operation was also significantly lower than that before operation [(9.27±4.29) time, (18.27±3.07)FFT group was basically same as FF control and MD control (P=0.660 and P=0.489) and it was basically same to the success frequency in Y-maze learning before operation (P=0.700).CONCLUSION: The learning of Y-maze in rats with damaged marginal division of striatum was remarkably reduced and there was no obvious change in learning and memory between the rats with fimbria-fornix transaction and without transaction. It is verified that marginal division of striatum can reflex complex learning and memory behavior in electric Y-maze test, which cannot be achieved in hippocampus. It is further explained the difference of the two divisions in controlling learning and memory in cerebrum, the marginal division of striatum is able to control hippocampus in learning and memory function.