CAJ | 학술논문

为了研究益生菌发酵乳无菌提取物对食源性有害微生物(空肠弯曲杆菌)有毒基因表达的抑制作用,以利用空肠弯曲杆菌的有毒基因flaA σ28启动子和无启动子的质粒pRYluxCDABE联结构建的生物冷光转基因模型为试验材料,通过检测生物发光特性的方法,研究了空肠弯曲杆菌有毒基因flaA表达与益生菌(5种双岐杆菌,6 种乳酸杆菌)发酵乳无菌提取物之间的关系.结果表明,益生菌发酵乳无菌提取物对空肠弯曲杆菌flaA σ28启动子的活性有显著的抑制作用(P《0.05),并可显著抑制空肠弯曲杆菌有害基因flaA的表达.
위료연구익생균발효유무균제취물대식원성유해미생물(공장만곡간균)유독기인표체적억제작용,이이용공장만곡간균적유독기인flaA σ28계동자화무계동자적질립pRYluxCDABE련결구건적생물랭광전기인모형위시험재료,통과검측생물발광특성적방법,연구료공장만곡간균유독기인flaA표체여익생균(5충쌍기간균,6 충유산간균)발효유무균제취물지간적관계.결과표명,익생균발효유무균제취물대공장만곡간균flaA σ28계동자적활성유현저적억제작용(P《0.05),병가현저억제공장만곡간균유해기인flaA적표체.
To study the inhibition of germfree extracts derived from milk fermented by lactic acid bacteria (LAB) on virulence gene flaA expression of foodborne pathogen bacteria Campylobacter jejuni,a luminescent phenotype fusion between the flaA σ28 promoter and a promoterless luxCDABE cassette carried on the plasmid pRYluxCDABE in Campylobacter jejuni was used.Through evaluation of the bioluminescence,the effect of germfree extracts derived from milk fermented by lactic acid bacteria (LAB) (5 Bifidobacterium strains and 6 Lactobacillus strains) was assessed on flaA σ28 promoter activity.The results showed that gremfree extracts of milk fermented by LAB were found to have a significant (P<0.05) inhibitive effect on the expression of flaA σ28 promoter,and down-regulate virulence gene flaA expression.