CAJ | 학술논문

膜蛋白质Orai1组成了一类被称为钙释放激活钙通道(CRAC)的离子通道,并且由相互作用的蛋白质STIM1作为其在内质网上的钙感受器.但是这类通道的调节机制还未研究透彻.通过串连亲和纯化STIM1-Orai1复合体,发现与之相互作用的内质网蛋白质RCN2.共聚焦显微术显示RCN2与STIM1在钙库排空前后完全共定位.对RCN2的EF hands结构突变体所作单细胞测钙,结果显示其对钙库操控通道电流特性有微弱影响.全内反射荧光显微术显示,RCN2以花环状围绕包围STIM1聚集堆,这提示RCN2在STIM1聚集中起到一种结构约束作用.
막단백질Orai1조성료일류피칭위개석방격활개통도(CRAC)적리자통도,병차유상호작용적단백질STIM1작위기재내질망상적개감수기.단시저류통도적조절궤제환미연구투철.통과천련친화순화STIM1-Orai1복합체,발현여지상호작용적내질망단백질RCN2.공취초현미술현시RCN2여STIM1재개고배공전후완전공정위.대RCN2적EF hands결구돌변체소작단세포측개,결과현시기대개고조공통도전류특성유미약영향.전내반사형광현미술현시,RCN2이화배상위요포위STIM1취집퇴,저제시RCN2재STIM1취집중기도일충결구약속작용.
STIM1 is recognized as an ER Ca2+ sensor of calcium release-activated calcium (CRAC) channel that is constructed by membrane protein Orai1, However, this regulatory system may also be regulated by other proteins. Reticulocalbin 2 (RCN2) was purified and identified from STIM1-Orai1 complex. Confocal microscopy revealed that RCN2 co-localized with STIM1 in ER before and after Ca2+ store depletion. Single cell [Ca2+]I measurements of RCN2 EF hands mutant showed slight influence on SOC electrophysiological characters. Furthermore, a novel collar form aggregation of RCN2 surrounding STIM1 clusters suggested that RCN2 potentially plays a role of structure maintenance in STIM1 clustering.