中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2009年
12期
1215-1217
,共3页
棘阿米巴%18S rDNA%基因型%分类
棘阿米巴%18S rDNA%基因型%分類
극아미파%18S rDNA%기인형%분류
Acanthamoeba%18S rDNA%genotype%classification
目的 对吉林延吉市自来水中分离的棘阿米巴分离株Acanthamoeba sp. CJY/W1 18S rDNA基因型鉴定.方法 从本地区自来水分离的Acanthamoeba sp. CJY/W1虫株中提取基因组18S rDNA,应用棘阿米巴属特意性引物PCR扩增.将扩增产物测序后用Clustal X和Genedoc软件进行序列分析,与基因库中已有T1至T12型序列进行比较并构建进化树. 结果分离的棘阿米巴分离株CJY/W1的18S rDNA全基因为2 252bp,18S rDNA基因分型最接近于T1型,但与T1型之间的基因差异为8%.结论 分离的CJY/W1株是不属于T1-T12的新的18S rDNA基因型,接近于T13(Acanthamoeba sp. UWC9,AF132134).
目的 對吉林延吉市自來水中分離的棘阿米巴分離株Acanthamoeba sp. CJY/W1 18S rDNA基因型鑒定.方法 從本地區自來水分離的Acanthamoeba sp. CJY/W1蟲株中提取基因組18S rDNA,應用棘阿米巴屬特意性引物PCR擴增.將擴增產物測序後用Clustal X和Genedoc軟件進行序列分析,與基因庫中已有T1至T12型序列進行比較併構建進化樹. 結果分離的棘阿米巴分離株CJY/W1的18S rDNA全基因為2 252bp,18S rDNA基因分型最接近于T1型,但與T1型之間的基因差異為8%.結論 分離的CJY/W1株是不屬于T1-T12的新的18S rDNA基因型,接近于T13(Acanthamoeba sp. UWC9,AF132134).
목적 대길림연길시자래수중분리적극아미파분리주Acanthamoeba sp. CJY/W1 18S rDNA기인형감정.방법 종본지구자래수분리적Acanthamoeba sp. CJY/W1충주중제취기인조18S rDNA,응용극아미파속특의성인물PCR확증.장확증산물측서후용Clustal X화Genedoc연건진행서렬분석,여기인고중이유T1지T12형서렬진행비교병구건진화수. 결과분리적극아미파분리주CJY/W1적18S rDNA전기인위2 252bp,18S rDNA기인분형최접근우T1형,단여T1형지간적기인차이위8%.결론 분리적CJY/W1주시불속우T1-T12적신적18S rDNA기인형,접근우T13(Acanthamoeba sp. UWC9,AF132134).
To identify the genotype of the 18S rDNA in Acanthamoeba species isolated from tap water inYanji city of Jilin province, the full genomic DNA sequence of the CJY/W1 strain of Acanthaqmoeba was amplified by PCR and then the amplified products were sequenced. The full-length of DNA sequence was analyzed with Clustal and Genedoe softwares. It was found that the full-length of gene of the Acanthamoeba CJY/W1 strain isolated from tap water was 2252 bps. The 18S rDNA genotype of this strain appeared to be near to the T1 type. But the percentage of the genetic difference between T1 type was 8%. According to the classification criteria of 18S rDNA, the genotype of the CJY/W1 strain might be a new one.
