CAJ | 학술논문

以邻苯二甲醛-9-芴甲基氯甲酸酯为衍生化试剂,对氨基酸进行柱前衍生,建立了35℃下,醋酸钠缓冲液、甲醇及乙腈为流动相,用Eclipse AAA氨基酸分析柱和二极管阵列检测器的氨基酸色谱分析方法,18种氨基酸在36 min内获得了较好的分离,在一定浓度范围内,氨基酸的峰面积与浓度的线性相关系数在0.990 6～0.999 8之间,相对标准偏差为1.96%～5.45%(n=5).并测定了马氏珠母贝肉蛋白中氨基酸的组成和含量.结果表明,马氏珠母贝肉蛋白中含有至少18种氨基酸,其中8种必须氨基酸总含量达39%以上,是一种优质的功能食品原料.
이린분이갑철-9-물갑기록갑산지위연생화시제,대안기산진행주전연생,건립료35℃하,작산납완충액、갑순급을정위류동상,용Eclipse AAA안기산분석주화이겁관진렬검측기적안기산색보분석방법,18충안기산재36 min내획득료교호적분리,재일정농도범위내,안기산적봉면적여농도적선성상관계수재0.990 6～0.999 8지간,상대표준편차위1.96%～5.45%(n=5).병측정료마씨주모패육단백중안기산적조성화함량.결과표명,마씨주모패육단백중함유지소18충안기산,기중8충필수안기산총함량체39%이상,시일충우질적공능식품원료.
A reversed-phase high-performance liquid chromatographic method for the determination of amino acids is described. It involves pre-column derivatization with o-phthalaldehyde and 9-flu-orenylmethylchloroformate (FOMC) for the mixture of 18 kinds of amino acids. HPLC separation of the derivatives was performed using an Eclipse AAA column with NaAc buffer, methanol and aceto-nitrile mobile phase system with DAD detector and at 35 ℃ in 36 min. The response was linear for all amino acids with a correlation coefficient of 0. 9906 -0. 9998, Method precisions (n = 5) for the different amino acids were between 2. 02% -5.45% (relative standard deviation, RSD). Using this method, 18 kinds of amino acids in protein of Pinctada martensii were determined. The results showed that there were at least 18 kinds of amino acids in proteins of Pinctada martensii in which the total quantity of 8 essential amino acids was about 39%. It can be concluded that the protein of Pinctada martensii is a good functional food material.