CAJ | 학술논문

利用紫外光接枝聚合丙烯酰胺(Acrylic amide,AAm)获得表面酰胺化的聚乳酸(PLA)膜,并考察了成骨细胞在酰胺化表面的黏附和增殖行为.结果表明,酰胺基的引入改善了PLA膜的表面亲水性,其表面水接触角由78°减少到56°,自由能由42.7mJ/m~2增大到51.4mJ/m~2;与对照组相比,成骨细胞在改性表面培养3 d后有大量的丝状伪足伸出,并且较快地进入了细胞分裂期,表明PLA膜表面的酰胺化能够促进细胞的黏附和增殖.
이용자외광접지취합병희선알(Acrylic amide,AAm)획득표면선알화적취유산(PLA)막,병고찰료성골세포재선알화표면적점부화증식행위.결과표명,선알기적인입개선료PLA막적표면친수성,기표면수접촉각유78°감소도56°,자유능유42.7mJ/m~2증대도51.4mJ/m~2;여대조조상비,성골세포재개성표면배양3 d후유대량적사상위족신출,병차교쾌지진입료세포분렬기,표명PLA막표면적선알화능구촉진세포적점부화증식.
Acrylic amide (AAm) was polymerized onto polylactide (PLA) film via UV irradiation to achieve amidation surface, furthermore the adhesion and proliferation of osteoblast on this surface was investigated.The results show that the hydrophilicity of PLA film is improved by introduction amido: the contact angle Decreases from 78° to 56° and the free energy increases from 42. 7 mJ/m~2 to 51.4 mJ/m~2. Compared with the control, osteoblast spread out more filopodia after 3 d incubation on amidation surface, and it entered cell division phase more quickly, which indicated that the surface amidation could promote the cell's adhesion and proliferation on the PLA film.