CAJ | 학술논문

分离提取纯化海带中的有特殊补碘功效的有机活性碘,确定其结构,为科学补碘提供正确依据.方法步骤是:水浸提后,超微过滤除去大分子杂质;等电沉淀富集有机活性碘;活性炭柱特异性吸附分离后,纸层析分离单体组分;获得的单体组分经过熔点测定、质谱分析、红外光谱分析,并与DIT(3,5-二碘酪氨酸)标准品对比,确定其结构.结果发现,海带浸提过程中,存在三碘(无机碘、有机碘、总碘)溶出平衡曲线,50～55℃浸提温度下,浸提周期10～12 h最为合理;pH 5.0～5.3的等电沉淀,可以富集79%的海带有机碘;纸层析,得到80 mg海带有机活性碘纯品;熔点测定结果,该品的熔点为(323±2)℃,与DIT标准品的(323±1.5)℃接近;海带中有特殊补碘功效的有机活性碘质谱分析、红外光谱分析结果,与DIT标准品分析结构吻合,确定这种活性碘为3,5-二碘酪氨酸.
분리제취순화해대중적유특수보전공효적유궤활성전,학정기결구,위과학보전제공정학의거.방법보취시:수침제후,초미과려제거대분자잡질;등전침정부집유궤활성전;활성탄주특이성흡부분리후,지층석분리단체조분;획득적단체조분경과용점측정、질보분석、홍외광보분석,병여DIT(3,5-이전락안산)표준품대비,학정기결구.결과발현,해대침제과정중,존재삼전(무궤전、유궤전、총전)용출평형곡선,50～55℃침제온도하,침제주기10～12 h최위합리;pH 5.0～5.3적등전침정,가이부집79%적해대유궤전;지층석,득도80 mg해대유궤활성전순품;용점측정결과,해품적용점위(323±2)℃,여DIT표준품적(323±1.5)℃접근;해대중유특수보전공효적유궤활성전질보분석、홍외광보분석결과,여DIT표준품분석결구문합,학정저충활성전위3,5-이전락안산.
The target of this manuscript was to extract, purify and identify an organic iodide with the iodine supplementation activity from kelp. The maximum yields of organic iodine and total iodine were obtained when the kelp was extracted in 50～55 ℃ for 10～12 h and then 79% of organic iodine was recovered from kelp in the isoelectric precipitation in pH 5. 0 - 5. 3. At last, 80 mg pure organic active iodide was acquired through paper chromatography. By compared with that of the standard material and determined by mass chromatogram and infra-red spectrogram, the purified compound with iodine complementing function was identified as 3,5-diiodo-l-tyrosine (DIT).