中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2010年
4期
604-606
,共3页
罗兰%高淑娟%王淑美%梁生旺%李淑芳%李建生
囉蘭%高淑娟%王淑美%樑生旺%李淑芳%李建生
라란%고숙연%왕숙미%량생왕%리숙방%리건생
脑脉通%葛根素%大豆苷%大豆苷元%芹精基葛根素%3'-甲氧基葛根素%HPLC
腦脈通%葛根素%大豆苷%大豆苷元%芹精基葛根素%3'-甲氧基葛根素%HPLC
뇌맥통%갈근소%대두감%대두감원%근정기갈근소%3'-갑양기갈근소%HPLC
Maomaitong Granules%puerafin%daidzin%daidzein%daizein-8-capiosy(1→6)glucoside%3-methoxyl-puerafin%HPLC
目的:建立脑脉通有效部位中葛根异黄酮类成分的含量测定方法.方法:采用高效液相色谱法,流动相为甲醇-水-冰醋酸,梯度洗脱,流速为1.0 mL/min,检测波长为250 nm.结果:葛根素的平均加样回收率为100.89%,RSD=2.10%,芹糖基葛根素的平均加样回收率为100.23%,RSD=1.06%,3'-甲氧基葛根素的平均加样回收率为101.04%,RSD=1.92%,大豆苷的平均加样回收率为99.82%,RSD=2.02%,大豆苷元的平均加样回收率为102.06%,RSD=1.34%.结论:该方法操作简便、易行,可用于脑脉通中葛根异黄酮的含量测定.
目的:建立腦脈通有效部位中葛根異黃酮類成分的含量測定方法.方法:採用高效液相色譜法,流動相為甲醇-水-冰醋痠,梯度洗脫,流速為1.0 mL/min,檢測波長為250 nm.結果:葛根素的平均加樣迴收率為100.89%,RSD=2.10%,芹糖基葛根素的平均加樣迴收率為100.23%,RSD=1.06%,3'-甲氧基葛根素的平均加樣迴收率為101.04%,RSD=1.92%,大豆苷的平均加樣迴收率為99.82%,RSD=2.02%,大豆苷元的平均加樣迴收率為102.06%,RSD=1.34%.結論:該方法操作簡便、易行,可用于腦脈通中葛根異黃酮的含量測定.
목적:건립뇌맥통유효부위중갈근이황동류성분적함량측정방법.방법:채용고효액상색보법,류동상위갑순-수-빙작산,제도세탈,류속위1.0 mL/min,검측파장위250 nm.결과:갈근소적평균가양회수솔위100.89%,RSD=2.10%,근당기갈근소적평균가양회수솔위100.23%,RSD=1.06%,3'-갑양기갈근소적평균가양회수솔위101.04%,RSD=1.92%,대두감적평균가양회수솔위99.82%,RSD=2.02%,대두감원적평균가양회수솔위102.06%,RSD=1.34%.결론:해방법조작간편、역행,가용우뇌맥통중갈근이황동적함량측정.
AIM:To establish an HPLC method for determing content of puerafin isoflavones in effective parts of Naomaitong Granules.METHoDS:Gradient elution with methanol-water-glacial acetic acid Wag used as the mobile phase.The flow rate Was 1.0 mL/min,and the detection wavelength Wag set at 250 nm.RESULTS:Theaverage recoveries of puerarin,daizein-8-capiosy(1→6)glucoside,3-methoxyl-puerarin,daidzin,daidzein were 100.89%(RSD=2.1%),100.23%(RSD=1.06%),101.04%(RSD=1.92%),99.82%(RSD=2.02%),102.06%(RSD=1.34%)respectively.CONCLUSIoN:The method is simple,accurate and can be used for quality control of the effective parts in Naomaitong Granules.
