CAJ | 학술논문

肥胖及其相关的疾病影响了越来越多的人.在哺乳动物中调节脂肪代谢的因子和脂肪代谢途径在线虫中也是保守存在的,因此线虫是脂肪研究的良好动物模型.在研究线虫脂肪代谢中,存在多种脂肪染色方法,而不同方法所表征的脂肪含量存在一定的差异,通过各种染色方法检测基因突变或者饥饿引起的脂肪含量变化.实验结果表明,采用油红、苏丹黑、尼罗红染料的固定染色法均能够正确地表征线虫的贮存脂肪.
비반급기상관적질병영향료월래월다적인.재포유동물중조절지방대사적인자화지방대사도경재선충중야시보수존재적,인차선충시지방연구적량호동물모형.재연구선충지방대사중,존재다충지방염색방법,이불동방법소표정적지방함량존재일정적차이,통과각충염색방법검측기인돌변혹자기아인기적지방함량변화.실험결과표명,채용유홍、소단흑、니라홍염료적고정염색법균능구정학지표정선충적저존지방.
Obesity and its related diseases are affecting an increasing number of people.Many key fat regulatory genes and fat metabolism pathways found in mammals are conserved in C.elegans.Therefore,C.elegans is a powerful genetic model for fat biology research.Different methods were used to quantitate the fat stores,and the results turn out different.According to the test in fixed worms during fasting or gene mutation,the dye such as Oil Red O,Sudan Black B,Nile Red and C1-BODIPY-C12 could quantitate fat stores correctly.