中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
2期
129-131
,共3页
张琳琳%郭桅%黄震浩%李皓雲%段俊丽
張琳琳%郭桅%黃震浩%李皓雲%段俊麗
장림림%곽외%황진호%리호운%단준려
皮质醇%人脐静脉内皮细胞%一氧化氮%碱性成纤维细胞生长因子%基质衍生因子%缺氧诱导因子
皮質醇%人臍靜脈內皮細胞%一氧化氮%堿性成纖維細胞生長因子%基質衍生因子%缺氧誘導因子
피질순%인제정맥내피세포%일양화담%감성성섬유세포생장인자%기질연생인자%결양유도인자
Cortisol%Human umbilical vein endothelial cell%Nitric oxide%Basic fibroblast growth factor%Stromal cell derived factor-1%Hypoxia-inducible factor
目的 探讨皮质醇抑制血管新生的机制.方法 将体外培养G0/G1期的人脐静脉内皮细胞(HUVEC)分为皮质醇组(无血清RPMI1640培养液中加入皮质醇)和对照组(仅用无血清RPMI1640培养液),细胞计数试剂盒(CCK-8)法检测细胞存活率,细胞迁移实验观察皮质醇对细胞迁移的影响,酶联免疫吸附法测定细胞中人碱性成纤维细胞生长因子(bFGF)和基质细胞衍生因子1(SDF1)含量,蛋白质印迹法测定细胞缺氧诱导因子1α(HIF-1α)的表达.结果 皮质醇组细胞活力与对照组相比无明显差异；细胞迁移数目明显少于对照组,差异有统计学意义[ (618 ±117)个比(1200 ±57)个,P=0.00011]；bFGF、SDF1与NO水平明均明显低于对照组[bFGF为(69.7 ±5.7) ng/L比(99.3±11.0) ng/L,P＜0.01；SDF1为(10.2 ±2.1)ng/L比(12.4 ±2.5)ng/L,P＜0.05；NO为(58.5±10.3)μmol/L比(97.3±6.2)μmol/L,P=0.03407].结论 皮质醇对HUVEC细胞活力无明显抑制作用,但可以明显抑制其迁移功能,并降低细胞中NO、bFGF、SDF1的含量,这可能是皮质醇抑制HUVEC迁移的作用机制.
目的 探討皮質醇抑製血管新生的機製.方法 將體外培養G0/G1期的人臍靜脈內皮細胞(HUVEC)分為皮質醇組(無血清RPMI1640培養液中加入皮質醇)和對照組(僅用無血清RPMI1640培養液),細胞計數試劑盒(CCK-8)法檢測細胞存活率,細胞遷移實驗觀察皮質醇對細胞遷移的影響,酶聯免疫吸附法測定細胞中人堿性成纖維細胞生長因子(bFGF)和基質細胞衍生因子1(SDF1)含量,蛋白質印跡法測定細胞缺氧誘導因子1α(HIF-1α)的錶達.結果 皮質醇組細胞活力與對照組相比無明顯差異；細胞遷移數目明顯少于對照組,差異有統計學意義[ (618 ±117)箇比(1200 ±57)箇,P=0.00011]；bFGF、SDF1與NO水平明均明顯低于對照組[bFGF為(69.7 ±5.7) ng/L比(99.3±11.0) ng/L,P＜0.01；SDF1為(10.2 ±2.1)ng/L比(12.4 ±2.5)ng/L,P＜0.05；NO為(58.5±10.3)μmol/L比(97.3±6.2)μmol/L,P=0.03407].結論 皮質醇對HUVEC細胞活力無明顯抑製作用,但可以明顯抑製其遷移功能,併降低細胞中NO、bFGF、SDF1的含量,這可能是皮質醇抑製HUVEC遷移的作用機製.
목적 탐토피질순억제혈관신생적궤제.방법 장체외배양G0/G1기적인제정맥내피세포(HUVEC)분위피질순조(무혈청RPMI1640배양액중가입피질순)화대조조(부용무혈청RPMI1640배양액),세포계수시제합(CCK-8)법검측세포존활솔,세포천이실험관찰피질순대세포천이적영향,매련면역흡부법측정세포중인감성성섬유세포생장인자(bFGF)화기질세포연생인자1(SDF1)함량,단백질인적법측정세포결양유도인자1α(HIF-1α)적표체.결과 피질순조세포활력여대조조상비무명현차이；세포천이수목명현소우대조조,차이유통계학의의[ (618 ±117)개비(1200 ±57)개,P=0.00011]；bFGF、SDF1여NO수평명균명현저우대조조[bFGF위(69.7 ±5.7) ng/L비(99.3±11.0) ng/L,P＜0.01；SDF1위(10.2 ±2.1)ng/L비(12.4 ±2.5)ng/L,P＜0.05；NO위(58.5±10.3)μmol/L비(97.3±6.2)μmol/L,P=0.03407].결론 피질순대HUVEC세포활력무명현억제작용,단가이명현억제기천이공능,병강저세포중NO、bFGF、SDF1적함량,저가능시피질순억제HUVEC천이적작용궤제.
Objective To investigate the effects of cortisol on the migration of human umbilical vein endothelial cells(HUVEC).Methods The viability of the cells was determined by using cell counting kit-8 (CCK-8).Transwell_boydom system was used to test the migration of human umbilical vein endothelial cell induced by cortisol.Enzyme-linked immunosorbent assay(ELISA) method was used to determine the effect of cortisol on the expression ofBasic fibroblast growth factor(bFGF) and stromal cell derived factor-1 (SDF1).The expression of HIFlα was analyzed with Western blotting.Results It showed that cortisol itself had no significant inhibition on cell viability of HUVEC but significantly inhibited HUVEC migration and decreased the expression of bFGF and SDF1.There were no differences of expression ofHypoxia-inducible factor ( HIF1 α ) between control and cortisol groups.Conclusion Cortisol can decrease the expression of bFGF and SDF1 in HUVEC and inhibit the migration of HUVEC.
