CAJ | 학술논문

目的研究Toll样受体(TLR)9基因单核苷酸多态性(SNP)与HBV感染临床转归的相关性.方法应用实时荧光定量PCR方法检测96例慢性乙型重型肝炎患者、156例慢性乙型肝炎患者和151例HBV感染自发清除者TLR9基因启动子区A-1923C、T-1486C、T-1237C 3个位点的SNP分型,分析各位点的基因型及组间差异.计量资料采用方差分析,计数资料采用χ2或Fisher精确概率法检验.结果 TLR9基因A-1923C位点AC基因型在慢性乙型肝炎组的频率(3.8%)低于HBV感染自发清除组(11.3%)(χ2=6.082,P＜0.05),但在慢性乙型重型肝炎组(8.3%)与HBV感染自发清除组间基因型频率分布没有统计学差异(χ2=0.552,P＞0.05);重型肝炎组与自发清除组、慢性乙型肝炎组与自发清除组间T-1486C和T-1237C位点的基因型频率分布差异无统计学意义(χ2值分别为1.534和0.745,P值均＞0.05).结论 TLR9基因A-1923C位点AC基因型与HBV感染的自发清除存在相关性,但与HBV感染的慢性化和重症化无明显相关性;T-1486C、T-1237C位点的基因多态性与HBV感染结局之间没有相关性.
목적 연구Toll양수체(TLR)9기인단핵감산다태성(SNP)여HBV감염림상전귀적상관성.방법 응용실시형광정량PCR방법검측96례만성을형중형간염환자、156례만성을형간염환자화151례HBV감염자발청제자TLR9기인계동자구A-1923C、T-1486C、T-1237C 3개위점적SNP분형,분석각위점적기인형급조간차이.계량자료채용방차분석,계수자료채용χ2혹Fisher정학개솔법검험.결과 TLR9기인A-1923C위점AC기인형재만성을형간염조적빈솔(3.8%)저우HBV감염자발청제조(11.3%)(χ2=6.082,P＜0.05),단재만성을형중형간염조(8.3%)여HBV감염자발청제조간기인형빈솔분포몰유통계학차이(χ2=0.552,P＞0.05);중형간염조여자발청제조、만성을형간염조여자발청제조간T-1486C화T-1237C위점적기인형빈솔분포차이무통계학의의(χ2치분별위1.534화0.745,P치균＞0.05).결론 TLR9기인A-1923C위점AC기인형여HBV감염적자발청제존재상관성,단여HBV감염적만성화화중증화무명현상관성;T-1486C、T-1237C위점적기인다태성여HBV감염결국지간몰유상관성.
Objective To investigate whether the clinical outcomes of HBV infection are associated with single nucleotide polymorphisms of Toll-like receptor (TLR) 9 gene promoter region.Methods The polymorphisms of three positions at TLR9 gene promoter region including A-1923C, T-1486C and T-1237C were detected using real-time fluorescence quantitative polymerase chain reaction in 96 patients with severe chronic hepatitis B, 156 patients with chronic hepatitis B and 151 cases of HBV spontaneous clearance, then the differences between the groups were analyzed.Analysis of variance was performed for measurement data,and χ2 test or Fisher exact probability test were used for enumeration data.Results The frequency of AC genotype at TLR9 gene A-1923C site in chronic hepatitis B group was 3.8%, which was significantly lower than that in HBV spontaneous clearance group (11.3%) (χ2=6.082, P ＜ 0.05), but no significant difference was found between severe chronic hepatitis B group (8.3%) and HBV spontaneous clearance group (χ2=0.552, P ＞0.05).No significant differences of genotype distribution were found between chronic severe hepatitis B group and HBV spontaneous clearance group , chronic hepatitis B group and HBV spontaneous clearance group at polymorphism sites of T-1486C and T-1237C (χ2=1.534 and 0.745, P ＞ 0.05).Conclusions Genotype AC at TLR9 gene A-1923C site is associated with HBV spontaneous clearance, but not correlated with chronic HBV infection and liver failure; there is no correlation of polymorphisms in T-1486C and T-1237C at TLR9 gene promoter region with the clinical outcomes of HBV infection.