CAJ | 학술논문

目的探讨以痘苗病毒载体表达的甲肝病毒培养合适的细胞系和重组病毒灭活以及甲肝病毒抗原保存条件.方法将构建成功的表达甲肝抗原的重组痘苗病毒分别接种于K4、143、HEL、Hep-2和Vero等细胞,用ELISA测定重组甲肝抗原的表达产量和不同方法灭活重组病毒后的抗原活性.结果在K4、143和HEL细胞表达重组甲肝抗原的产量略优于Hep-2和Vero细胞,重组病毒经β-丙内酯和甲醛灭活后,甲肝抗原活性不变,制备的重组甲肝抗原稳定性好.结论痘苗病毒表达载体可以提高甲肝抗原表达效率,具有十分广阔的应用前景.
목적 탐토이두묘병독재체표체적갑간병독배양합괄적세포계화중조병독멸활이급갑간병독항원보존조건.방법 장구건성공적표체갑간항원적중조두묘병독분별접충우K4、143、HEL、Hep-2화Vero등세포,용ELISA측정중조갑간항원적표체산량화불동방법멸활중조병독후적항원활성.결과 재K4、143화HEL세포표체중조갑간항원적산량략우우Hep-2화Vero세포,중조병독경β-병내지화갑철멸활후,갑간항원활성불변,제비적중조갑간항원은정성호.결론 두묘병독표체재체가이제고갑간항원표체효솔,구유십분엄활적응용전경.
Objective To find a suitable cell line for hepatitis A antigen expressed by vaccinia virus vector and to find a way of inactivation and preservation of the HAV recombinant antigen.Methods Series of cell lines such as K4,143,HEL,Hep-2 and Vero were inoculated with vaccinia virus that can express HAV recombinant antigen.ELISA was used to determine the contents of expression antigen.The chracteration of the HAV antigen expressed by vaccinia virus was then analyzed after it was treated with different methods.Results The expression of HAV recombinant antigen in K4,143 and HEL cell lines was a little more than expression in Hep-2 and Vero cell lines.The antigenicity is obviously higher when HAV recombinant antigen was inactivated by β-propiolactone other than it was inactivated by formalin.It was best to preserve the prepared HAV recombinant antigen under -40℃ condition.Conclusions The application of vaccinia virus vector in hepatitis A antigen preparation was very useful and promising.