CAJ | 학술논문

目的了解新疆地区乙肝患者HBV基因型的分布及其与临床表现的关系.方法从2000余份血清中筛选出HBsAg阳性血清200份.利用巢式PCR法扩增血清中HBV DNA S基因进行测序并分型;统计分析基因型分布及与临床表现的关系.结果新疆HBV有B、C、D三个基因型,其中B基因型10例(7.8%),C基因型58例(45.7%),D基因型59例(46.5%).C基因型中汉族51例(87.9%),D型中汉族27例(45.7%),维吾尔族24例(40.7%).结论新疆地区HBV基因型以C、D型为主,少量为B型.C型以汉族为主,D型中汉族和维吾尔族占多数.C型与更严重的疾病及转归有关.
목적 료해신강지구을간환자HBV기인형적분포급기여림상표현적관계.방법 종2000여빈혈청중사선출HBsAg양성혈청200빈.이용소식PCR법확증혈청중HBV DNA S기인진행측서병분형;통계분석기인형분포급여림상표현적관계.결과 신강HBV유B、C、D삼개기인형,기중B기인형10례(7.8%),C기인형58례(45.7%),D기인형59례(46.5%).C기인형중한족51례(87.9%),D형중한족27례(45.7%),유오이족24례(40.7%).결론 신강지구HBV기인형이C、D형위주,소량위B형.C형이한족위주,D형중한족화유오이족점다수.C형여경엄중적질병급전귀유관.
Objective To determine the main genotype of hepatitis B virus (HBV) in Xinjiang.Methods 200 HBsAg positive serum specimens were detected from more than 2000 serum of Xinjiang inhabitants, and HBV S gene was detected by using nPCR amplifying, and compared with the standard S region HBV nucleotide sequences of genotypes A-H retrieved from GenBank, then analyzed by MEGA3 and SAS3 software. Result Gene in 127 (63.5%) serum specimens was detected from 200 samples. Among 127 serum specimens, 10 (7.8%) was genotype B, 58 (45.7%) was genotype C, and 59 (46.5%) was genotype D. Han People were 51 (87.9%) in genotype D and 27(45.7%) in genotype D, Uygur were 24 in genotype D. Conclusion Genotype B(40.7%), C and D have been found in Xinjiang. Genotype C was more prone to causing severe liver disease.