中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
4期
257-259
,共3页
李蕾%费永亮%伊瑶%陈斯勇%毕胜利
李蕾%費永亮%伊瑤%陳斯勇%畢勝利
리뢰%비영량%이요%진사용%필성리
肝炎病毒,乙型%基因型%人种群%临床医学
肝炎病毒,乙型%基因型%人種群%臨床醫學
간염병독,을형%기인형%인충군%림상의학
Hepatitis B virus%Genotype%Ethnic groups%Clinical medicine
目的 了解新疆地区乙肝患者HBV基因型的分布及其与临床表现的关系.方法 从2000余份血清中筛选出HBsAg阳性血清200份.利用巢式PCR法扩增血清中HBV DNA S基因进行测序并分型;统计分析基因型分布及与临床表现的关系.结果 新疆HBV有B、C、D三个基因型,其中B基因型10例(7.8%),C基因型58例(45.7%),D基因型59例(46.5%).C基因型中汉族51例(87.9%),D型中汉族27例(45.7%),维吾尔族24例(40.7%).结论 新疆地区HBV基因型以C、D型为主,少量为B型.C型以汉族为主,D型中汉族和维吾尔族占多数.C型与更严重的疾病及转归有关.
目的 瞭解新疆地區乙肝患者HBV基因型的分佈及其與臨床錶現的關繫.方法 從2000餘份血清中篩選齣HBsAg暘性血清200份.利用巢式PCR法擴增血清中HBV DNA S基因進行測序併分型;統計分析基因型分佈及與臨床錶現的關繫.結果 新疆HBV有B、C、D三箇基因型,其中B基因型10例(7.8%),C基因型58例(45.7%),D基因型59例(46.5%).C基因型中漢族51例(87.9%),D型中漢族27例(45.7%),維吾爾族24例(40.7%).結論 新疆地區HBV基因型以C、D型為主,少量為B型.C型以漢族為主,D型中漢族和維吾爾族佔多數.C型與更嚴重的疾病及轉歸有關.
목적 료해신강지구을간환자HBV기인형적분포급기여림상표현적관계.방법 종2000여빈혈청중사선출HBsAg양성혈청200빈.이용소식PCR법확증혈청중HBV DNA S기인진행측서병분형;통계분석기인형분포급여림상표현적관계.결과 신강HBV유B、C、D삼개기인형,기중B기인형10례(7.8%),C기인형58례(45.7%),D기인형59례(46.5%).C기인형중한족51례(87.9%),D형중한족27례(45.7%),유오이족24례(40.7%).결론 신강지구HBV기인형이C、D형위주,소량위B형.C형이한족위주,D형중한족화유오이족점다수.C형여경엄중적질병급전귀유관.
Objective To determine the main genotype of hepatitis B virus (HBV) in Xinjiang.Methods 200 HBsAg positive serum specimens were detected from more than 2000 serum of Xinjiang inhabitants, and HBV S gene was detected by using nPCR amplifying, and compared with the standard S region HBV nucleotide sequences of genotypes A-H retrieved from GenBank, then analyzed by MEGA3 and SAS3 software. Result Gene in 127 (63.5%) serum specimens was detected from 200 samples. Among 127 serum specimens, 10 (7.8%) was genotype B, 58 (45.7%) was genotype C, and 59 (46.5%) was genotype D. Han People were 51 (87.9%) in genotype D and 27(45.7%) in genotype D, Uygur were 24 in genotype D. Conclusion Genotype B(40.7%), C and D have been found in Xinjiang. Genotype C was more prone to causing severe liver disease.