中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2010年
12期
1160-1163
,共4页
高扬%李心河%刘延红%夏洪印%王惠敏%王传新
高颺%李心河%劉延紅%夏洪印%王惠敏%王傳新
고양%리심하%류연홍%하홍인%왕혜민%왕전신
紫癜,血小板减少性,特发性%膜蛋白质类%T淋巴细胞,调节性%流式细胞术
紫癜,血小闆減少性,特髮性%膜蛋白質類%T淋巴細胞,調節性%流式細胞術
자전,혈소판감소성,특발성%막단백질류%T림파세포,조절성%류식세포술
Purpura,thrombocytopenic,idiopathic%Membrane proteins%T-Lymphocytes,regulatory%Flow cytometry
目的 探讨Tim-3、Th17细胞及Treg细胞在ITP患者中的表达及其临床意义.方法 应用流式细胞术检测42例ITP患者与39名健康对照者Th17细胞及Treg细胞的表达;采用ELISA法检测外周血血浆中IL-17、IFN-γ和IL-4的表达;应用RT-PCR方法 检测Tim-3、IFN-γ、IL-4、T-bet等细胞因子和转录因子的mRNA表达.结果 ITP患者Th17细胞比例为(2.41±1.43)%,明显高于健康对照组的(1.08±0.59)%,差异有统计学意义(t=5.35,P<0.05);ITP患者Treg细胞比例为(1.64±0.74)%,明显低于健康对照组的(3.12±0.52)%,差异有统计学意义(t=10.33,P<0.05).IL-17在ITP患者血浆中的表达水平为(14.42±6.37)ng/L,健康对照组为(13.91±4.47)ng/L,差异无统计学意义(t=0.42,P>0.05);而ITP患者血浆IFN-γ含量为(55.74±15.25)ng/L,较健康对照组的(31.33±12.99)ng/L明显升高,IL-4含量为(7.42±1.50)ng/L,较健康对照组的(18.17±5.19)ng/L明显降低,差异均有统计学意义(t=7.72、2.87,P均<0.05).ITP患者的T-bet mRNA和IFN-γmRNA表达水平明显升高,分别为健康对照组的(3.34±1.32)倍和(8.57±3.44)倍,差异有统计学意义(t=6.41、13.21,P<0.05);而Tim-3 mRNA和IL-4 mRNA表达水平明显下降,分别为健康对照的(0.29±0.15)倍和(0.25±0.15)侪,差异均有统计学意义(t=9.61、10.02,P<0.05).结论 Th17/Treg细胞亚群比例失调和Tim-3的表达下降可能是ITP发生和发展的重要决定因素.
目的 探討Tim-3、Th17細胞及Treg細胞在ITP患者中的錶達及其臨床意義.方法 應用流式細胞術檢測42例ITP患者與39名健康對照者Th17細胞及Treg細胞的錶達;採用ELISA法檢測外週血血漿中IL-17、IFN-γ和IL-4的錶達;應用RT-PCR方法 檢測Tim-3、IFN-γ、IL-4、T-bet等細胞因子和轉錄因子的mRNA錶達.結果 ITP患者Th17細胞比例為(2.41±1.43)%,明顯高于健康對照組的(1.08±0.59)%,差異有統計學意義(t=5.35,P<0.05);ITP患者Treg細胞比例為(1.64±0.74)%,明顯低于健康對照組的(3.12±0.52)%,差異有統計學意義(t=10.33,P<0.05).IL-17在ITP患者血漿中的錶達水平為(14.42±6.37)ng/L,健康對照組為(13.91±4.47)ng/L,差異無統計學意義(t=0.42,P>0.05);而ITP患者血漿IFN-γ含量為(55.74±15.25)ng/L,較健康對照組的(31.33±12.99)ng/L明顯升高,IL-4含量為(7.42±1.50)ng/L,較健康對照組的(18.17±5.19)ng/L明顯降低,差異均有統計學意義(t=7.72、2.87,P均<0.05).ITP患者的T-bet mRNA和IFN-γmRNA錶達水平明顯升高,分彆為健康對照組的(3.34±1.32)倍和(8.57±3.44)倍,差異有統計學意義(t=6.41、13.21,P<0.05);而Tim-3 mRNA和IL-4 mRNA錶達水平明顯下降,分彆為健康對照的(0.29±0.15)倍和(0.25±0.15)儕,差異均有統計學意義(t=9.61、10.02,P<0.05).結論 Th17/Treg細胞亞群比例失調和Tim-3的錶達下降可能是ITP髮生和髮展的重要決定因素.
목적 탐토Tim-3、Th17세포급Treg세포재ITP환자중적표체급기림상의의.방법 응용류식세포술검측42례ITP환자여39명건강대조자Th17세포급Treg세포적표체;채용ELISA법검측외주혈혈장중IL-17、IFN-γ화IL-4적표체;응용RT-PCR방법 검측Tim-3、IFN-γ、IL-4、T-bet등세포인자화전록인자적mRNA표체.결과 ITP환자Th17세포비례위(2.41±1.43)%,명현고우건강대조조적(1.08±0.59)%,차이유통계학의의(t=5.35,P<0.05);ITP환자Treg세포비례위(1.64±0.74)%,명현저우건강대조조적(3.12±0.52)%,차이유통계학의의(t=10.33,P<0.05).IL-17재ITP환자혈장중적표체수평위(14.42±6.37)ng/L,건강대조조위(13.91±4.47)ng/L,차이무통계학의의(t=0.42,P>0.05);이ITP환자혈장IFN-γ함량위(55.74±15.25)ng/L,교건강대조조적(31.33±12.99)ng/L명현승고,IL-4함량위(7.42±1.50)ng/L,교건강대조조적(18.17±5.19)ng/L명현강저,차이균유통계학의의(t=7.72、2.87,P균<0.05).ITP환자적T-bet mRNA화IFN-γmRNA표체수평명현승고,분별위건강대조조적(3.34±1.32)배화(8.57±3.44)배,차이유통계학의의(t=6.41、13.21,P<0.05);이Tim-3 mRNA화IL-4 mRNA표체수평명현하강,분별위건강대조적(0.29±0.15)배화(0.25±0.15)제,차이균유통계학의의(t=9.61、10.02,P<0.05).결론 Th17/Treg세포아군비례실조화Tim-3적표체하강가능시ITP발생화발전적중요결정인소.
Objective To investigate the expression and the role of Tim-3 and Th-17 in ITP patients and to research their clinical application. Methods Total 42 active ITP patients and 39 healthy donors were recruited in this research. The expressions of Th17 and CD4+ CD25+ Treg were measured with flow cytometer. IL-17, IFN-γ levels as well as IL-4 plasma levels were determined by ELISA. The mRNA expression of Tim-3, IFN-γ, IL-4 and T-bet were measured using RT-PCR in all samples. Results The expression of Th17 cells in ITP patients was (2.41 ± 1.43 )%, which was significantly higher than control group ( 1.08 ± 0.59)% ( t = 5.35, P < 0.05 ). But the percentage of Treg in ITP patients was ( 1.64 ±0.74)%, which was lower than control group (3.12 ±0.52)% (t = 10.33, P <0.05). The levels of IL-17 in plasma of ITP and controls were ( 14.42 ±6.37) ng/L and ( 13.91 ±4.47) ng/L respectively (t =0.42, P > 0.05). The level of IFN-γin plasma of ITP was (55.74 ± 15.25 ) ng/L, which was higher than control group (31.33 ± 12.99) ng/L (t = 7.72, P < 0.05 ). The level of IL-4 in the plasma of ITP was (7.42 ± 1.50) ng/L, which was lower than controls ( 18.17 ± 5.19) ng/L ( t = 12.87, P ＜ 0.05 ). Both IFN-γand T-bet mRNA levels were up-regulated in active ITP patients by the factor ( 8.57 ± 3.44 ) -fold and (3.34 ± 1.32)-fold than control group (t = 13.21,6.41 ,P <0.05). The decreases observed in IL-4 and Tim-3 were (0.25 ±0.15 )-fold and (0.29 ±0.15)-fold respectively in ITP patients compared with control group (t=10.02,9.61,P＜0.05 ). Conclusion The imbalance of Th17/Treg and the decrease of Tim-3 might be important determinants in the evolution of ITP.
