CAJ | 학술논문

目的探讨依地酸(ethylenediamine tetraacetic acid,EDTA)与环丙沙星联用对豚鼠体内铜绿假单胞菌生物被膜感染的清除作用.方法吸入法建立豚鼠肺部铜绿假单胞菌生物被膜感染模型；EDTA腹腔注射、环丙沙星雾化吸入,单独及联合作用于感染后豚鼠；平板计数法对比药物作用前后生物被膜菌落数变化,HE染色及电镜观察肺部病理变化.结果 EDTA、环丙沙星单独及联合均对生物被膜有杀菌作用(P＜0.05),联合应用的效果最好,使菌落数由105 CFU/g降至10 CFU/g(t=24.67,P＜0.05)且肺部组织病理改变明显减轻,生物被膜结构也被破坏.结论 EDTA与环丙沙星联合应用对体内肺部铜绿假单胞菌生物被膜感染有显著清除作用.
목적 탐토의지산(ethylenediamine tetraacetic acid,EDTA)여배병사성련용대돈서체내동록가단포균생물피막감염적청제작용.방법 흡입법건립돈서폐부동록가단포균생물피막감염모형；EDTA복강주사、배병사성무화흡입,단독급연합작용우감염후돈서；평판계수법대비약물작용전후생물피막균락수변화,HE염색급전경관찰폐부병리변화.결과 EDTA、배병사성단독급연합균대생물피막유살균작용(P＜0.05),연합응용적효과최호,사균락수유105 CFU/g강지10 CFU/g(t=24.67,P＜0.05)차폐부조직병리개변명현감경,생물피막결구야피파배.결론 EDTA여배병사성연합응용대체내폐부동록가단포균생물피막감염유현저청제작용.
Objective To investigate the effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm in vivo.Methods Pseudomonas aeruginosa was inhaled into the lung of guinea pigs and colonized,formed biofilm.After 7 days,the model was treated with ciprofloxacin,EDTA alone,or a combination of both for 7 days.The number of colony in the lungs is measured by agar plate.The pathological change of the lung is observed by hematoxylin and eosin (HE) staining and scanning electron microscope.Results EDTA combined with ciprofloxacin make the number of bacteria in the lungs reduced from l05 CFU/g to 10 CFU/g(t =24.67,P＜0.05),the lung lesion was less-sever histophathologically.Conclusion The combination of EDTA with ciprofloxacin has significant activity to remove mucoid PA biofilm in vivo.